Exam 14: Regulation of Gene Expression in Bacteria and Bacteriophages

Antisense control of translation involves the regulation of which enzyme produced by bacterial insertion sequence IS10?

Which enzyme is responsible for integration of a temperate phage into a host genome?

What is the process by which a bacterium switches from a lysogenic to lytic cycle?

In λ phage, entry into the ________ cycle requires transcription of late genes.

In negative control, what molecule would you expect to find bound to the operator if there is no transcription?

Regulation of transcription of bacterial genes takes place at which two levels?

Suppose a $\lambda$ phage infected a protease-deficient bacterial cell. Would the lytic or lysogenic life cycle be favored? Why?

Which region of the λ phage genome enables the linear chromosome to circularize when it enters a host cell?

Which enzyme is the product of the lacZ gene?

In the presence of glucose, where is the lac repressor bound?

Binding of which complex increases the ability of RNA polymerase to transcribe the lac operon?

The genetic switch controlling whether a bacterium enters the lytic or lysogenic cycle relies on the binding of which two proteins?

The enzyme β-galactosidase catalyzes what reaction?

Bacterial translation is inhibited by ________ RNA, which is complementary to a portion of a specific mRNA.

Mutations of trpL decrease the efficiency of transcriptional regulation by disrupting the formation of which stem-loop structure?

Which of these haploid strains produces β-galactosidase constitutively but does not produce permease?

Which proteins facilitate RNA polymerase binding at promoters?

What is the purpose of the lac operon, and under what circumstances is it activated? Describe the structure of the lac operon, including the repressor protein, structural genes, and regulatory regions of the operon. What is the role of catabolite repression in the lac operon?

In the trp operon, where is the attenuator region located?

If a corepressor is inhibited, what effect would you expect to see in the operon?