Question 1

Which of these haploid strains produces permease but does not produce β-galactosidase?

Accepted Answer

A) I - P+ O+ Z+ Y+ 
B) I+ P+ O+ Z - Y+ 
C) I - P+ O+ Z - Y - 
D) I+ P+ O - Z+ Y+ 
E) I - P+ O+ Z+ Y - 
A) I - P+ O+ Z+ Y+ 
B) I+ P+ O+ Z - Y+ 
C) I - P+ O+ Z - Y - 
D) I+ P+ O - Z+ Y+ 
E) I - P+ O+ Z+ Y -

Question 2

What would be the consequences to a $\lambda$ phage that lysogenized in a recA− bacterial cell? Explain why this would occur.

Accepted Answer

Induction (the termination of lysogeny a

Question 3

Which sigma factor, encoded by the rpoH gene, is active at high temperatures?

Accepted Answer

A) σ70 
B) σ32 
C) σ45 
D) σ37 
E) σ50 
A) σ70 
B) σ32 
C) σ45 
D) σ37 
E) σ50

Question 4

Catabolite repression refers to the repression of the lac operon in the presence of which catabolite?

Accepted Answer

The answer of Catabolite repression refers to the repression of...

Question 5

You want to design a repressor protein mutant. Which protein domain is the best target for preventing binding of the corepressor?

Accepted Answer

A) DNA-binding domain 
B) allosteric domain 
C) promoter domain 
D) helix-turn-helix domain 
E) activator binding site 
A) DNA-binding domain 
B) allosteric domain 
C) promoter domain 
D) helix-turn-helix domain 
E) activator binding site

Question 6

Which of the following mutants are noninducible?

Accepted Answer

A) lacIS 
B) lacI - 
C) lacI+ 
D) lacOC 
E) lacO+ 
A) lacIS 
B) lacI - 
C) lacI+ 
D) lacOC 
E) lacO+

Question 7

During the attenuation of the trp operon, which stem loop leads to polycistronic mRNA synthesis during tryptophan starvation?

Accepted Answer

A) 1-3 (antitermination)stem loop 
B) 3-4 (termination)stem loop 
C) 1-2 (pause)stem loop 
D) 2-3 (antitermination)stem loop 
E) 2-4 (termination)stem loop 
A) 1-3 (antitermination)stem loop 
B) 3-4 (termination)stem loop 
C) 1-2 (pause)stem loop 
D) 2-3 (antitermination)stem loop 
E) 2-4 (termination)stem loop

Question 8

A bacterium is unable to transport lactose into the cell to be broken down. Which gene is likely mutated in this bacterium?

Accepted Answer

A) lacZ 
B) lacY 
C) lacI 
D) lacP 
E) lacO 
A) lacZ 
B) lacY 
C) lacI 
D) lacP 
E) lacO

Question 9

What are the two active sites or "domains" on a repressor protein?

Accepted Answer

DNA-bindin

Question 10

Changing conformation at the active site as a result of binding a substance at a different site is known as ________.

Accepted Answer

The answer of Changing conformation at the active site as...

Question 11

Bacteria grown at 45°C initiate expression of which two types of proteins?

Accepted Answer

heat shock

Question 12

Which region of the lac operon would you target if you want to disrupt the -10 and -35 consensus sequences?

Accepted Answer

A) lacZ 
B) lacY 
C) lacI 
D) lacP 
E) lacO 
A) lacZ 
B) lacY 
C) lacI 
D) lacP 
E) lacO

Question 13

In E. coli, the cya gene encodes adenylate cyclase. Will cells that are cya− be able to grow on medium that contains lactose as the sole carbon source? Why or why not?

Accepted Answer

No. Metabolically, cells that are cya⁻ wo

Question 14

Most of the regulation of gene expression in bacteria occurs at the ________ level.

Accepted Answer

The answer of Most of the regulation of gene expression...

Which of these haploid strains produces permease but does not produce β-galactosidase?

What would be the consequences to a $\lambda$ phage that lysogenized in a recA⁻ bacterial cell? Explain why this would occur.

Which sigma factor, encoded by the rpoH gene, is active at high temperatures?

Catabolite repression refers to the repression of the lac operon in the presence of which catabolite?

You want to design a repressor protein mutant. Which protein domain is the best target for preventing binding of the corepressor?

Which of the following mutants are noninducible?

During the attenuation of the trp operon, which stem loop leads to polycistronic mRNA synthesis during tryptophan starvation?

A bacterium is unable to transport lactose into the cell to be broken down. Which gene is likely mutated in this bacterium?

What are the two active sites or "domains" on a repressor protein?

Changing conformation at the active site as a result of binding a substance at a different site is known as ________.

Bacteria grown at 45°C initiate expression of which two types of proteins?

Which region of the lac operon would you target if you want to disrupt the -10 and -35 consensus sequences?

In E. coli, the cya gene encodes adenylate cyclase. Will cells that are cya⁻ be able to grow on medium that contains lactose as the sole carbon source? Why or why not?

Most of the regulation of gene expression in bacteria occurs at the ________ level.

The Molecular Basis of Heredity, Variation, and Evolution

Transmission Genetics

Cell Division and Chromosome Heredity

Gene Interaction

Genetic Linkage and Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophages

Dna Structure and Replication

Molecular Biology and Transcription and Rna Processing

The Molecular Biology of Translation

The Integration of Genetic Approaches: Understanding Sickle Cell Disease

Chromosome Structure

Gene Mutation, Dna Repair, and Homologous Recombination

Chromosome Aberrations and Transposition

Regulation of Gene Expression in Eukaryotes

Forward Genetics and Recombinant Dna Technology

Applications of Recombinant Dna Technology and Reverse Genetics

Genomics: Genetics From a Whole-Genome Perspective

Cytoplasmic Inheritance and the Evolution of Organelle Genomes

Developmental Genetics

Genetic Analysis of Quantitative Traits

Population Genetics and Evolution

Filters

Exam 14: Regulation of Gene Expression in Bacteria and Bacteriophages

Which of these haploid strains produces permease but does not produce β-galactosidase?

What would be the consequences to a λ\lambdaλ phage that lysogenized in a recA− bacterial cell? Explain why this would occur.

Which sigma factor, encoded by the rpoH gene, is active at high temperatures?

Catabolite repression refers to the repression of the lac operon in the presence of which catabolite?

You want to design a repressor protein mutant. Which protein domain is the best target for preventing binding of the corepressor?

Which of the following mutants are noninducible?

During the attenuation of the trp operon, which stem loop leads to polycistronic mRNA synthesis during tryptophan starvation?

A bacterium is unable to transport lactose into the cell to be broken down. Which gene is likely mutated in this bacterium?

What are the two active sites or "domains" on a repressor protein?

Changing conformation at the active site as a result of binding a substance at a different site is known as ________.

Bacteria grown at 45°C initiate expression of which two types of proteins?

Which region of the lac operon would you target if you want to disrupt the -10 and -35 consensus sequences?

In E. coli, the cya gene encodes adenylate cyclase. Will cells that are cya− be able to grow on medium that contains lactose as the sole carbon source? Why or why not?

Most of the regulation of gene expression in bacteria occurs at the ________ level.

The Molecular Basis of Heredity, Variation, and Evolution

Transmission Genetics

Cell Division and Chromosome Heredity

Gene Interaction

Genetic Linkage and Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophages

Dna Structure and Replication

Molecular Biology and Transcription and Rna Processing

The Molecular Biology of Translation

The Integration of Genetic Approaches: Understanding Sickle Cell Disease

Chromosome Structure

Gene Mutation, Dna Repair, and Homologous Recombination

Chromosome Aberrations and Transposition

Regulation of Gene Expression in Eukaryotes

Forward Genetics and Recombinant Dna Technology

Applications of Recombinant Dna Technology and Reverse Genetics

Genomics: Genetics From a Whole-Genome Perspective

Cytoplasmic Inheritance and the Evolution of Organelle Genomes

Developmental Genetics

Genetic Analysis of Quantitative Traits

Population Genetics and Evolution

Filters

What would be the consequences to a $\lambda$ phage that lysogenized in a recA⁻ bacterial cell? Explain why this would occur.

In E. coli, the cya gene encodes adenylate cyclase. Will cells that are cya⁻ be able to grow on medium that contains lactose as the sole carbon source? Why or why not?