Question 1

DNA helicase inhibitors are well studied as potential drug targets. What would you expect to see if DNA helicase activity is inhibited?

Accepted Answer

A) The replisome complex would not assemble on the oriC region. 
B) The DNA cannot bend, so hydrogen bonds in the 13-mer region of oriC remain intact. 
C) Helicase catalyzes ATP hydrolysis and DNA strands separation, so the helix cannot be unwound and strands will not separate. 
D) Helicase carries the SSB protein to the open region of DNA, so hydrolysis and strand separation will not occur. 
E) Helicase prevents reannealing of the separated strands, so strands would quickly reanneal and DNA replication cannot proceed. 
A) The replisome complex would not assemble on the oriC region. 
B) The DNA cannot bend, so hydrogen bonds in the 13-mer region of oriC remain intact. 
C) Helicase catalyzes ATP hydrolysis and DNA strands separation, so the helix cannot be unwound and strands will not separate. 
D) Helicase carries the SSB protein to the open region of DNA, so hydrolysis and strand separation will not occur. 
E) Helicase prevents reannealing of the separated strands, so strands would quickly reanneal and DNA replication cannot proceed.

Question 2

The large, complex aggregation of proteins and enzymes that assembles at the replication fork is known as the ________.

Accepted Answer

The answer of The large, complex aggregation of proteins and...

Question 3

The extraordinary accuracy of the DNA polymerase III enzyme lies in its ability to "proofread" newly synthesized DNA, a function of the enzyme's __________.

Accepted Answer

A) 3′-to-5′ exonuclease activity 
B) 5′-to-3′ exonuclease activity 
C) 3′-to-5′ polymerase activity 
D) 5′-to-3′ polymerase activity 
E) 3′-to-5′ helicase activity 
A) 3′-to-5′ exonuclease activity 
B) 5′-to-3′ exonuclease activity 
C) 3′-to-5′ polymerase activity 
D) 5′-to-3′ polymerase activity 
E) 3′-to-5′ helicase activity

Question 4

Which of the following would you find in a Sanger sequencing reaction but not in a polymerase chain reaction?

Accepted Answer

A) DNA template 
B) DNA polymerase 
C) dNTPs 
D) ddNTPs 
E) DNA primer 
A) DNA template 
B) DNA polymerase 
C) dNTPs 
D) ddNTPs 
E) DNA primer

Question 5

During DNA replication in E. coli, how does the cell prevent the DNA strands from following their natural tendency to seek maximum stability and reanneal?

Accepted Answer

SSB preven

Question 6

What is the molecular diameter of the DNA helix (in angstroms)?

Accepted Answer

The answer of What is the molecular diameter of the...

Question 7

Avery, Macleod, and McCarty expanded on Griffith's experiment to prove that DNA is the hereditary molecule required for transformation. What treatment of the heat-killed SIII bacteria extract resulted in the mouse living?

Accepted Answer

A) destroying type SIII lipids and polysaccharides 
B) destroying type SIII DNA with DNase 
C) destroying type SIII RNA with RNase 
D) destroying type SIII proteins with protease 
E) the control group (null treatment), in which all components are intact 
A) destroying type SIII lipids and polysaccharides 
B) destroying type SIII DNA with DNase 
C) destroying type SIII RNA with RNase 
D) destroying type SIII proteins with protease 
E) the control group (null treatment), in which all components are intact

Question 8

What is required for DNA polymerase to initiate DNA strand synthesis?

Accepted Answer

A) a short DNA primer synthesized by the enzyme primase 
B) a short RNA primer synthesized by the enzyme primase 
C) ATP and a short DNA primer synthesized by the enzyme topoisomerase 
D) ATP and a short RNA primer synthesized by the enzyme topoisomerase 
E) DNA polymerase initiates DNA strand synthesis without requiring any additional enzymes. 
A) a short DNA primer synthesized by the enzyme primase 
B) a short RNA primer synthesized by the enzyme primase 
C) ATP and a short DNA primer synthesized by the enzyme topoisomerase 
D) ATP and a short RNA primer synthesized by the enzyme topoisomerase 
E) DNA polymerase initiates DNA strand synthesis without requiring any additional enzymes.

Question 9

What fraction of the nucleotides in a double-stranded DNA molecule are cytosine and thymine (% C and % T added together)?

Accepted Answer

A) 25 % 
B) 50 % 
C) 75 % 
D) 100% 
E) It depends on the DNA sequence 
A) 25 % 
B) 50 % 
C) 75 % 
D) 100% 
E) It depends on the DNA sequence

Question 10

Describe the DNA helix structure proposed by Watson and Crick. What is the diameter of the helix and the distance between bases? What types of bonds are involved in stabilizing the DNA backbone, and what types of bonds are found between complementary base pairs? Why would a sequence containing high concentrations of G and C require more energy to break the bonds?

Accepted Answer

The DNA model contains two DNA polymer s

Question 11

The process by which Pneumococcus transfers DNA between living type RII and heat-killed type SIII cells is known as __________.

Accepted Answer

A) transformation 
B) replication 
C) transduction 
D) ligation 
E) conjugation 
A) transformation 
B) replication 
C) transduction 
D) ligation 
E) conjugation

Question 12

Where would you expect to find telomerase activity?

Accepted Answer

A) At the end of a chromosome in a normal healthy eukaryotic body (somatic)cell. 
B) At the end of a chromosome in a cancerous eukaryotic body cell. 
C) On the leading strand of DNA in a normal bacterial cell. 
D) On the lagging strand of DNA in a normal bacterial cell. 
E) At the centromere of a chromosome in a healthy eukaryotic reproductive cell. 
A) At the end of a chromosome in a normal healthy eukaryotic body (somatic)cell. 
B) At the end of a chromosome in a cancerous eukaryotic body cell. 
C) On the leading strand of DNA in a normal bacterial cell. 
D) On the lagging strand of DNA in a normal bacterial cell. 
E) At the centromere of a chromosome in a healthy eukaryotic reproductive cell.

Question 13

In the Hershey-Chase experiment, what was labeled by growing bacteriophage in 32P-containing medium?

Accepted Answer

A) ghost phage particles 
B) proteins 
C) lipids 
D) DNA 
E) RNA 
A) ghost phage particles 
B) proteins 
C) lipids 
D) DNA 
E) RNA

Question 14

What do the DNA sequences found at the origins of replication signify?

Accepted Answer

binding re

Question 15

What did Okazaki's discovery of the short segments of DNA reveal about DNA replication on the lagging strand?

Accepted Answer

It is disc

Question 16

Which arrow(s)point(s)to the location of helicase in the diagram shown?

Accepted Answer

A) A 
B) B 
C) C 
D) A and E 
E) B and D 
A) A 
B) B 
C) C 
D) A and E 
E) B and D

Question 17

The major and minor grooves of DNA are features of the helix that can be attributed to _______, which involves tight packing of DNA bases in a duplex.

Accepted Answer

The answer of The major and minor grooves of DNA...

DNA helicase inhibitors are well studied as potential drug targets. What would you expect to see if DNA helicase activity is inhibited?

The large, complex aggregation of proteins and enzymes that assembles at the replication fork is known as the ________.

The extraordinary accuracy of the DNA polymerase III enzyme lies in its ability to "proofread" newly synthesized DNA, a function of the enzyme's __________.

Which of the following would you find in a Sanger sequencing reaction but not in a polymerase chain reaction?

During DNA replication in E. coli, how does the cell prevent the DNA strands from following their natural tendency to seek maximum stability and reanneal?

What is the molecular diameter of the DNA helix (in angstroms)?

Avery, Macleod, and McCarty expanded on Griffith's experiment to prove that DNA is the hereditary molecule required for transformation. What treatment of the heat-killed SIII bacteria extract resulted in the mouse living?

What is required for DNA polymerase to initiate DNA strand synthesis?

What fraction of the nucleotides in a double-stranded DNA molecule are cytosine and thymine (% C and % T added together)?

The process by which Pneumococcus transfers DNA between living type RII and heat-killed type SIII cells is known as __________.

Where would you expect to find telomerase activity?

In the Hershey-Chase experiment, what was labeled by growing bacteriophage in ³²P-containing medium?

What do the DNA sequences found at the origins of replication signify?

What did Okazaki's discovery of the short segments of DNA reveal about DNA replication on the lagging strand?

Which arrow(s)point(s)to the location of helicase in the diagram shown?

The major and minor grooves of DNA are features of the helix that can be attributed to _______, which involves tight packing of DNA bases in a duplex.

The Molecular Basis of Heredity, Variation, and Evolution

Transmission Genetics

Cell Division and Chromosome Heredity

Gene Interaction

Genetic Linkage and Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophages

Molecular Biology and Transcription and Rna Processing

The Molecular Biology of Translation

The Integration of Genetic Approaches: Understanding Sickle Cell Disease

Chromosome Structure

Gene Mutation, Dna Repair, and Homologous Recombination

Chromosome Aberrations and Transposition

Regulation of Gene Expression in Bacteria and Bacteriophages

Regulation of Gene Expression in Eukaryotes

Forward Genetics and Recombinant Dna Technology

Applications of Recombinant Dna Technology and Reverse Genetics

Genomics: Genetics From a Whole-Genome Perspective

Cytoplasmic Inheritance and the Evolution of Organelle Genomes

Developmental Genetics

Genetic Analysis of Quantitative Traits

Population Genetics and Evolution

Filters

Exam 7: Dna Structure and Replication

DNA helicase inhibitors are well studied as potential drug targets. What would you expect to see if DNA helicase activity is inhibited?

The large, complex aggregation of proteins and enzymes that assembles at the replication fork is known as the ________.

The extraordinary accuracy of the DNA polymerase III enzyme lies in its ability to "proofread" newly synthesized DNA, a function of the enzyme's __________.

Which of the following would you find in a Sanger sequencing reaction but not in a polymerase chain reaction?

During DNA replication in E. coli, how does the cell prevent the DNA strands from following their natural tendency to seek maximum stability and reanneal?

What is the molecular diameter of the DNA helix (in angstroms)?

Avery, Macleod, and McCarty expanded on Griffith's experiment to prove that DNA is the hereditary molecule required for transformation. What treatment of the heat-killed SIII bacteria extract resulted in the mouse living?

What is required for DNA polymerase to initiate DNA strand synthesis?

What fraction of the nucleotides in a double-stranded DNA molecule are cytosine and thymine (% C and % T added together)?

The process by which Pneumococcus transfers DNA between living type RII and heat-killed type SIII cells is known as __________.

Where would you expect to find telomerase activity?

In the Hershey-Chase experiment, what was labeled by growing bacteriophage in 32P-containing medium?

What do the DNA sequences found at the origins of replication signify?

What did Okazaki's discovery of the short segments of DNA reveal about DNA replication on the lagging strand?

Which arrow(s)point(s)to the location of helicase in the diagram shown?

The major and minor grooves of DNA are features of the helix that can be attributed to _______, which involves tight packing of DNA bases in a duplex.

The Molecular Basis of Heredity, Variation, and Evolution

Transmission Genetics

Cell Division and Chromosome Heredity

Gene Interaction

Genetic Linkage and Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophages

Molecular Biology and Transcription and Rna Processing

The Molecular Biology of Translation

The Integration of Genetic Approaches: Understanding Sickle Cell Disease

Chromosome Structure

Gene Mutation, Dna Repair, and Homologous Recombination

Chromosome Aberrations and Transposition

Regulation of Gene Expression in Bacteria and Bacteriophages

Regulation of Gene Expression in Eukaryotes

Forward Genetics and Recombinant Dna Technology

Applications of Recombinant Dna Technology and Reverse Genetics

Genomics: Genetics From a Whole-Genome Perspective

Cytoplasmic Inheritance and the Evolution of Organelle Genomes

Developmental Genetics

Genetic Analysis of Quantitative Traits

Population Genetics and Evolution

Filters

In the Hershey-Chase experiment, what was labeled by growing bacteriophage in ³²P-containing medium?