Exam 3: Nucleic Acid Structure and Function

Explain the two methods used to determine that plasmid cloning has been successful.

During eukaryotic DNA condensation, nucleosomes are packed together to form

Several histones can bind to one DNA molecule, forming a repeating unit called a

When a gene sequence is cloned using mRNA, the mRNA is isolated from the cell and converted into a double-stranded sequence using which enzyme?

Foreign DNA fragments can be inserted into plasmids using

When DNA is sequenced, which analytical technique is used to separate the chain-terminated DNA fragments?

When DNA is transcribed into RNA, the strand has the complementary sequence to the transcribed RNA.

In the eukaryotic cell, the NONcoding sequences on a gene are referred to as

Outline the steps involved in DNA condensation.

The proteins that bind to DNA in a sequence-independent manner are

If DNA is not cleaved, the total linking number will remain the same, even though the twist and writhe can change. Using one or two complete sentences, explain why the total linking number does not change.

Predict how type I topoisomerases change the supercoil region.

When a gene sequence is cloned using mRNA, which enzyme is used to seal the single-strand gaps left behind in the second strand of DNA?

As DNA unwinds and denatures, absorbance is predicted to

When DNA is transcribed into RNA, the strand has the same base sequences as the RNA transcript.

Which of the following expresses the relationship among the linking number, twist, and writhe?

Identify the phase of PCR amplification where the primer is annealed.

The exons of a single gene often encode for different functional domains of a protein, which can result in

Histones participate in the process of supercoiling DNA. Using the figure below, outline the three steps in this process labeled A, B, and C.

Compare and contrast the arrangements of A-DNA, B-DNA, and Z-DNA.