Question 1

The interior stacking of the DNA bases in the double helix provides stability through

Accepted Answer

A)  hydrophobic and van der Waals interactions. 
B)  hydrophilic and van der Waals interactions. 
C)  hydrophilic and ion-dipole interactions. 
D)  hydrophobic and ion-dipole interactions. 
A)  hydrophobic and van der Waals interactions. 
B)  hydrophilic and van der Waals interactions. 
C)  hydrophilic and ion-dipole interactions. 
D)  hydrophobic and ion-dipole interactions.

Question 2

DNA strands containing 20 base pairs, 40 base pairs, and 60 base pairs were denatured and the results were graphed below. Identify the curve from the 60 base pair DNA strand.

Accepted Answer

A)  A 
B)  B 
C)  C 
D)  Not enough information is included to determine the curve. 
A)  A 
B)  B 
C)  C 
D)  Not enough information is included to determine the curve.

Question 3

The A-T content for several DNA strands is reported below. Which strand would have the highest Tm?

Accepted Answer

A)  40% A-T 
B)  50% A-T 
C)  60% A-T 
D)  70% A-T 
A)  40% A-T 
B)  50% A-T 
C)  60% A-T 
D)  70% A-T

Question 4

Identify the phase of PCR amplification where DNA synthesis occurs.

Accepted Answer

A)  A 
B)  B 
C)  C 
D)  D 
A)  A 
B)  B 
C)  C 
D)  D

Question 5

RNA only contains which of the following bases?

Accepted Answer

A)  thymine 
B)  adenine 
C)  uracil 
D)  guanine 
A)  thymine 
B)  adenine 
C)  uracil 
D)  guanine

Question 6

Chargaff's rule is that the amount of

Accepted Answer

A)  A = G and the amount of C = T. 
B)  A = C = G = T. 
C)  A = T and the amount of C = G. 
D)  A = C and the amount of G = T. 
A)  A = G and the amount of C = T. 
B)  A = C = G = T. 
C)  A = T and the amount of C = G. 
D)  A = C and the amount of G = T.

Question 7

Polycistronic genes that contain a coding sequence for proteins that are only involved in one biochemical process are called

Accepted Answer

A)  exons. 
B)  operons. 
C)  introns. 
D)  promoters. 
A)  exons. 
B)  operons. 
C)  introns. 
D)  promoters.

Question 8

The figure below shows part of the primary structure of DNA. Identify the nucleotide.

Accepted Answer

A)  A 
B)  B 
C)  C 
D)  D 
A)  A 
B)  B 
C)  C 
D)  D

Question 9

Why does DNA contain thymine instead of uracil?

Accepted Answer

A common reaction in cells is for cytosi

Question 10

How does the organization of prokaryotic genes differ from the organization of eukaryotic genes?

Accepted Answer

Prokaryotic genes can either be monocist

Question 11

The ability to monitor the denaturation of DNA using absorbance is referred to as

Accepted Answer

A)  annealing. 
B)  supercoiling. 
C)  melting temperature. 
D)  hyperchromic effect. 
A)  annealing. 
B)  supercoiling. 
C)  melting temperature. 
D)  hyperchromic effect.

Question 12

Two identical copies of replicated DNA that remain attached until cell division are referred to as

Accepted Answer

A)  telomeres. 
B)  centromeres. 
C)  kinetochores. 
D)  sister chromatids. 
A)  telomeres. 
B)  centromeres. 
C)  kinetochores. 
D)  sister chromatids.

Question 13

Less condensed, gene-rich chromatin is referred to as

Accepted Answer

A)  nucleosomes. 
B)  histones. 
C)  heterochromatin. 
D)  euchromatin. 
A)  nucleosomes. 
B)  histones. 
C)  heterochromatin. 
D)  euchromatin.

Question 14

Which method of analyzing RNA transcripts is considered to be an unbiased approach because it does not use a predetermined collection of complementary DNA sequence?

Accepted Answer

A)  plasmid cloning 
B)  viral transduction 
C)  RNA-seq 
D)  gene-expression microarrays 
A)  plasmid cloning 
B)  viral transduction 
C)  RNA-seq 
D)  gene-expression microarrays

Question 15

A double helix that crosses itself in a right-handed twist is referred to as a

Accepted Answer

A)  positive supercoil. 
B)  negative supercoil. 
C)  topoisomer. 
D)  linking number. 
A)  positive supercoil. 
B)  negative supercoil. 
C)  topoisomer. 
D)  linking number.

Question 16

Compare the primary and secondary structures of DNA.

Accepted Answer

The primary structure of DNA refers to t

Question 17

Compare and contrast the structure of euchromatin and heterochromatin. Explain how their structure relates to biological function.

Accepted Answer

Euchromatin is less condensed and more g

Question 18

Which protein is responsible for the proper separation of the chromosomes during cell division?

Accepted Answer

A)  telomeres 
B)  centromeres 
C)  kinetochores 
D)  sister chromatids 
A)  telomeres 
B)  centromeres 
C)  kinetochores 
D)  sister chromatids

Question 19

Why does the Tm increase as the G-C content increases?

Accepted Answer

G-C base pairs have more favor

Question 20

Identify the products when Sm al cleaves the following DNA sequence.

Accepted Answer

A) a 
B) b 
C) c 
D) d 
A) a 
B) b 
C) c 
D) d

Exam 3: Nucleic Acid Structure and Function

The interior stacking of the DNA bases in the double helix provides stability through

DNA strands containing 20 base pairs, 40 base pairs, and 60 base pairs were denatured and the results were graphed below. Identify the curve from the 60 base pair DNA strand.

The A-T content for several DNA strands is reported below. Which strand would have the highest Tm?

Identify the phase of PCR amplification where DNA synthesis occurs.

RNA only contains which of the following bases?

Chargaff's rule is that the amount of

Polycistronic genes that contain a coding sequence for proteins that are only involved in one biochemical process are called

The figure below shows part of the primary structure of DNA. Identify the nucleotide.

Why does DNA contain thymine instead of uracil?

How does the organization of prokaryotic genes differ from the organization of eukaryotic genes?

The ability to monitor the denaturation of DNA using absorbance is referred to as

Two identical copies of replicated DNA that remain attached until cell division are referred to as

Less condensed, gene-rich chromatin is referred to as

Which method of analyzing RNA transcripts is considered to be an unbiased approach because it does not use a predetermined collection of complementary DNA sequence?

A double helix that crosses itself in a right-handed twist is referred to as a

Compare the primary and secondary structures of DNA.

Compare and contrast the structure of euchromatin and heterochromatin. Explain how their structure relates to biological function.

Which protein is responsible for the proper separation of the chromosomes during cell division?

Why does the Tm increase as the G-C content increases?

Identify the products when Sm al cleaves the following DNA sequence.

Principles of Biochemistry

Physical Biochemistry: Energy Conversion, Water, and Membranes

Protein Structure

Methods in Protein Biochemistry

Protein Function

Enzyme Mechanisms

Cell Signaling Systems

Glycolysis: a Paradigm of Metabolic Regulation

The Citrate Cycle

Oxidative Phosphorylation

Photosynthesis

Carbohydrate Structure and Function

Carbohydrate Metabolism

Lipid Structure and Function

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Metabolic Integration

DNA Replication, Repair, and Recombination

RNA Synthesis, Processing, and Gene Silencing

Protein Synthesis, Posttranslational Modification, and Transport

Gene Regulation

Filters

The A-T content for several DNA strands is reported below. Which strand would have the highest T_m?

Why does the T_m increase as the G-C content increases?