Exam 17: Control of Gene Expression in Eukaryotes

Consider the way alternative splicing leads to the production of the small t antigen and the large T antigen in the mammalian virus SV40. What would be the result of a mutation that occurred in the gene that encodes the SF2 protein?

We learned in Chapter 17 that GAL3 protein binds to GAL80 and frees up GAL4 to bind to UAS and activate transcription of genes involved in galactose metabolism. Assuming that you did not have prior knowledge of GAL3 protein but knew about GAL4 and GAL80, what experiments would you perform to identify the GAL3 gene and additional genes involved in the regulation of GAL4?

Which of the following events is known to make DNA more sensitive to digestion by DNase I?

What would be the advantage of regulating gene expression at many levels rather than simply regulating at one level (such as at the start of transcription)?