Exam 13: Transcription

The following diagram represents DNA that is part of the RNA-coding sequence of a transcription unit. ATAGGCGATGCCA TATCCGCTACGGT The sequence of the RNA product of transcription is 5'-UGGCAUCGCCUAU-3'. Label the diagram of the DNA molecule to indicate which strand serves as the template strand. Also label the 5' and 3' ends of both strands.

If you wanted to express a eukaryotic gene in E. coli, would the normal promoter be sufficient for transcription? Why or why not?

Which of the following molecules is synthesized using nucleotides containing the bases adenine, guanine, cytosine, and uracil?

Diagram an active transcription unit as it would appear at high magnification. On the diagram indicate each of the following: (a) the DNA molecule (b) the RNA molecule that was initiated earliest (c) the 5' end of an RNA molecule (d) the 5' end of the template strand of DNA (e) the approximate location of the promoter (f) a molecule of RNA polymerase (g) an arrow indicating the direction of transcription

An in vitro transcription system transcribes a bacterial gene but terminates inefficiently. What is one possible problem?

If the DNA strand 5ʹ-GTACCGTC-3ʹ were used as a template, what would be the sequence of the transcribed RNA?

What is the RNA sequence transcribed from the DNA shown below?

On the DNA strands shown below, two RNA polymerase enzymes are using the top strand as a template. In the boxes, label the 5' and 3' ends of the DNA molecules and the RNA molecules being made. With arrows, indicate the directions, left to right or right to left, that the polymerases are moving.

Which of the following statements about RNA polymerase is NOT true?

Choose the BEST consensus sequence for the following set of nucleotide sequences. TAAGACGCCATGA AAAGTCGCAATCA AAAGTTCCGTTCA AGAGTTGCTATCA AGAGTTGCAATCA

The following diagram represents the transcription unit and corresponding RNA transcripts as "Christmas-tree-like" structures captured in the electron micrograph. - Which of the following statements CORRECTLY describes the orientation of the DNA and RNA transcript? (Select all that apply.)

Both strands of a DNA molecule are used as a template when which of the following molecules is synthesized?

What is the RNA sequence transcribed from the DNA shown below?

Compare rho-dependent and rho-independent transcription termination in terms of their use of RNA secondary structures.

Where are promoters usually located?

Which of the following statements is TRUE regarding transcription in most organisms?

An in vitro transcription system that contains a bacterial gene does not initiate transcription. What is one possible problem?

What is the function of general transcription factors?

Which of the following is not necessary for RNA polymerase to recognize the promoter of a bacterial gene?

What types of bonds are created between nucleotides during the process of transcription?