Question 1

Metaphase nuclei are typically required for accurate use of ________ ________FISH probes.

Accepted Answer

The answer of Metaphase nuclei are typically required for accurate...

Question 2

If a specimen is suspected to be a genetic mosaic, analysis should be repeated with a different stain to confirm the two cell populations.

Accepted Answer

A) True 
 B)False

Question 3

An advantage of indirectly labeled probes in FISH is that slides archived at -20° C for a year can be rewashed and stained with fresh labeling agent to revive the signal and allow rereading of the slides.

Accepted Answer

A) True 
 B)False

Question 4

Which specimen type(s)below can be used for FISH analysis?

Accepted Answer

A) Paraffin embedded thin tissue sections 
B) Blood/bone marrow smears 
C) Gametes 
D) Amniotic fluid 
E) All of the above 
A) Paraffin embedded thin tissue sections 
B) Blood/bone marrow smears 
C) Gametes 
D) Amniotic fluid 
E) All of the above

Question 5

Which of the following will cause poor probe hybridization efficiency?

Accepted Answer

A) Overdenaturing target DNA 
B) Underdenaturing target DNA 
C) Both A and B 
D) Exposure of the slide to cold after target DNA denaturation 
E) Dehydration of the slide after target DNA denaturation 
A) Overdenaturing target DNA 
B) Underdenaturing target DNA 
C) Both A and B 
D) Exposure of the slide to cold after target DNA denaturation 
E) Dehydration of the slide after target DNA denaturation

Metaphase nuclei are typically required for accurate use of FISH probes.

If a specimen is suspected to be a genetic mosaic, analysis should be repeated with a different stain to confirm the two cell populations.

An advantage of indirectly labeled probes in FISH is that slides archived at -20° C for a year can be rewashed and stained with fresh labeling agent to revive the signal and allow rereading of the slides.

Which specimen type(s)below can be used for FISH analysis?

Which of the following will cause poor probe hybridization efficiency?

What Is Immunology

Overview of the Immune System

Immunoglobulins

Infection and Immunity: Hostparasite Relationships

The Complement System

Hypersensitivity

Immunologic Detection of Infectious Diseases

Autoimmune Diseases and Immunodeficiency Disorders

Transplant Immunology

Tumor Immunology

Clinical Applications of Flow Cytometry

Hybridization Techniques

Amplification Techniques

Histocompatibility Laboratory Techniques and Applications

Filters

Exam 14: Molecular Applications in Cytogenetics

Metaphase nuclei are typically required for accurate use of ________ ________FISH probes.

If a specimen is suspected to be a genetic mosaic, analysis should be repeated with a different stain to confirm the two cell populations.

An advantage of indirectly labeled probes in FISH is that slides archived at -20° C for a year can be rewashed and stained with fresh labeling agent to revive the signal and allow rereading of the slides.

Which specimen type(s)below can be used for FISH analysis?

Which of the following will cause poor probe hybridization efficiency?

What Is Immunology

Overview of the Immune System

Immunoglobulins

Infection and Immunity: Hostparasite Relationships

The Complement System

Hypersensitivity

Immunologic Detection of Infectious Diseases

Autoimmune Diseases and Immunodeficiency Disorders

Transplant Immunology

Tumor Immunology

Clinical Applications of Flow Cytometry

Hybridization Techniques

Amplification Techniques

Histocompatibility Laboratory Techniques and Applications

Filters

Metaphase nuclei are typically required for accurate use of FISH probes.