Exam 11: Clinical Applications of Flow Cytometry

A transplant recipient showed signs of rejection, so she was treated with monoclonal anti- CD3 (OKT3). Flow cytometry was done on her blood after treatment with results as shown in the figure. The gate was set on lymphocytes. What is the best interpretation of that analysis given?

A patient presented with a very high white blood count with 90% lymphocytes. Flow cytometry analysis was done with the gate set on the lymphocytes. What is the best interpretation of the results below?

Describe how data are collected and displayed in flow cytometry.

Fluorochromes absorb light at one wavelength and then emit light at a shorter wavelength.

Once cells have been fixed in paraformaldehyde for flow cytometry analysis, they must be analyzed immediately or the cell surface markers will deteriorate.

When staining cells for flow cytometry, which technique would have the least amount of nonspecific staining?

Flow cytometry can be done to identify small malignant cell populations within a normal larger population. It is therefore especially important that specimens are

Which of the following changes are possible in malignant cells?

Although all human white cells possess the CD45 marker, immature cells possess less of it and stain dimly on flow cytometry analysis.

Serial measurements of ________ positive lymphocytes in HIV patients are done by flow cytometry to determine when prophylactic antibiotics should be given.

How many fluorochromes can the average flow cytometer detect?

How are cell surface antigens detected by a flow cytometer?

Which of the following cells should scatter the most light in the side angle?

In flow cytometry, the process by which the cell population to be analyzed is selected is called fluorescence compensation.

Cell analysis in flow cytometry can be done using fluorescent stains for nucleic acids such as DNA.

Describe both direct and indirect antigen staining in flow cytometry and the advantages and disadvantages of each.

What characteristic(s)of a cell can be determined by a flow cytometer?

A flow cytometry analysis was set up as follows. How can T helper cells be identified? Analysis of forward and side laser light scatter anti- CD3 labeled with green fluorochrome anti- CD4 labeled with red fluorochrome Anti- CD8 labeled with yellow fluorochrome anti- CD19 labeled with blue fluorochrome

When patients are put on the waiting list for a kidney transplant, they are tested for alloantibodies to HLA antigens when they are initially put on the list and then a second time just prior to the transplant.

In order for flow cytometry analysis to work properly, the cells must be dead in order to properly take up stain.