Exam 8: Genetic Analysis and Mapping in Bacteria and Bactierophages

Lysogeny is a process that occurs during transformation and conjugation.

If two different auxotrophic strains are placed in a liquid medium culture tube, prototrophic strains can sometimes be subsequently recovered. Name several mechanisms by which this is possible.

Explain the composition and use of minimal medium in the study of bacterial genetics.

In a bacterial cross in which the donor (Hfr) is a+b+ and the recipient strain (F -) is a -b -, it is expected that recombinant bacteria will all be a+b+.

What is the role of the F factor in bacterial recombination?

R plasmids often contain genes for antibody production.

What experimental observation demonstrated that genes are involved in the integration of foreign DNA into the bacterial chromosome?

Bacteria that are in a particular physiological state to become transformed are called ________.

How does an auxotroph differ from a prototroph?

Present the general structural features of a plasmid and give an example.

What is a form of recombination in bacteria that involves the F plasmid?

The clearing made by bacteriophages in a "lawn" of bacteria on an agar plate is called a ________.

What is the consequence of a mutation in the recA gene in bacteria?

What is a significant difference between a lytic and a lysogenic cycle?

What are the roles of the recBCD genes?

Describe how different strains of E. coli can reveal different linkage arrangements of genes in Hfr crosses.

Viral mutations and variants are often categorized by changes in host range and/or plaque morphology.

Compare and contrast bacteriophage lysis and lysogeny.

Transduction is a form of recombination in bacteria that involves

In general, what two methods are used to grow bacteria in the laboratory?