Question 1

Which of the following would be most difficult to detect using CGH?

Accepted Answer

A)  100kb duplications 
B)  inversions 
C)  aneuploidy 
D)  100kb deletions 
E)  50Mb deletions 
A)  100kb duplications 
B)  inversions 
C)  aneuploidy 
D)  100kb deletions 
E)  50Mb deletions B

Question 2

Probes for DNA microarrays are made using

Accepted Answer

A)  RNA blotting 
B)  Southern blotting 
C)  PCR 
D)  Electrophoresis 
E)  DNA sequencing 
A)  RNA blotting 
B)  Southern blotting 
C)  PCR 
D)  Electrophoresis 
E)  DNA sequencing C

Question 3

Which of the following is the correct sequence of events in sample preparation for microarray analysis?

Accepted Answer

A)  RNA extraction,reverse transcription,differential labelling,hybridization 
B)  RNA extraction,reverse transcription,hybridization,differential labelling 
C)  reverse transcription,RNA extraction,differential labelling,hybridization 
D)  differential labelling,RNA extraction,reverse transcription,hybridization 
E)  RNA extraction,differential labelling,hybridization,reverse transcription 
A)  RNA extraction,reverse transcription,differential labelling,hybridization 
B)  RNA extraction,reverse transcription,hybridization,differential labelling 
C)  reverse transcription,RNA extraction,differential labelling,hybridization 
D)  differential labelling,RNA extraction,reverse transcription,hybridization 
E)  RNA extraction,differential labelling,hybridization,reverse transcription A

Question 4

Design an experiment to identify genes associated with cancer,using methylome analysis.

Accepted Answer

Collect DNA from tumour tissues and norm

Question 5

Why are genome wide association studies (GWAS)preferable to traditional pedigree analysis for mapping genes associated with complex traits?

Accepted Answer

Pedigree analysis,especially for complex

Question 6

Bisulfite sequencing can be included under next-generation sequencing.

Accepted Answer

A) True 
 B)False

Question 7

Design an experiment to identify genes associated with cancer,using genome wide association studies.

Accepted Answer

Collect DNA samples from thousands of in

Question 8

Bisulfite sequencing relies on _______________ to detect ____________.

Accepted Answer

A)  methylation of cytosine;methylated DNA 
B)  immunoprecipitation of methylated DNA;promoter regions 
C)  conversion of cysteine to uracil;unmethylated DNA 
D)  conversion of cytosine to uracil;unmethylated DNA 
E)  immunoprecipitation of promoter regions;methylated DNA 
A)  methylation of cytosine;methylated DNA 
B)  immunoprecipitation of methylated DNA;promoter regions 
C)  conversion of cysteine to uracil;unmethylated DNA 
D)  conversion of cytosine to uracil;unmethylated DNA 
E)  immunoprecipitation of promoter regions;methylated DNA

Question 9

How are the probes for CGH different from probes made for gene expression analysis?

Accepted Answer

A)  Arrays for CGH are made using BAC probes. 
B)  Arrays for CGH are made using cDNA probes. 
C)  Arrays for CGH are made using synthetic oligonucleotide probes. 
D)  Arrays for CGH are made using PCR amplicon probes. 
E)  Arrays for CGH are made using miRNA probes. 
A)  Arrays for CGH are made using BAC probes. 
B)  Arrays for CGH are made using cDNA probes. 
C)  Arrays for CGH are made using synthetic oligonucleotide probes. 
D)  Arrays for CGH are made using PCR amplicon probes. 
E)  Arrays for CGH are made using miRNA probes.

Question 10

In genome analysis,presence of the Kozak signal is evidence of

Accepted Answer

A)  enhancer sites. 
B)  splicing sites. 
C)  binding sites for core transcriptional machinery. 
D)  the start of a coding region. 
E)  polyadenylation. 
A)  enhancer sites. 
B)  splicing sites. 
C)  binding sites for core transcriptional machinery. 
D)  the start of a coding region. 
E)  polyadenylation.

Question 11

How is CGH used in studying or treating cancer?

Accepted Answer

A)  Transcription factors that acts as tumour-suppressors can be identified by CGH. 
B)  Transcription factors that are more abundant in tumours can be identified by CGH. 
C)  Transcripts that are more abundant in tumours can be identified by CGH. 
D)  Trisomy 21 can be identified using CGH. 
E)  Deletions containing tumour-suppressor genes are often found in tumours and can be detected by CGH. 
A)  Transcription factors that acts as tumour-suppressors can be identified by CGH. 
B)  Transcription factors that are more abundant in tumours can be identified by CGH. 
C)  Transcripts that are more abundant in tumours can be identified by CGH. 
D)  Trisomy 21 can be identified using CGH. 
E)  Deletions containing tumour-suppressor genes are often found in tumours and can be detected by CGH.

Question 12

Haplotypes are

Accepted Answer

A)  genotypes of haploid organisms. 
B)  one half of a SNP genotype. 
C)  alleles made of the SNPs in a block of DNA. 
D)  genotypes made from gametic DNA. 
E)  ambiguous genotypes resulting from dominant markers. 
A)  genotypes of haploid organisms. 
B)  one half of a SNP genotype. 
C)  alleles made of the SNPs in a block of DNA. 
D)  genotypes made from gametic DNA. 
E)  ambiguous genotypes resulting from dominant markers.

Question 13

Antibodies specific to 5-methylcytosine can be used to precipitate DNA prior to array hybridization.

Accepted Answer

A) True 
 B)False

Question 14

Design an experiment to identify genes associated with cancer,using comparative genome hybridization.

Accepted Answer

Collect DNA from tumour tissues and norm

Question 15

ChIP-seq does not require a microarray for analysis.

Accepted Answer

A) True 
 B)False

Question 16

Almost all of a person's genetic diversity can be described using

Accepted Answer

A)  CGH. 
B)  a detailed pedigree. 
C)  a karyotype. 
D)  tag SNPs. 
E)  cDNA microarrays. 
A)  CGH. 
B)  a detailed pedigree. 
C)  a karyotype. 
D)  tag SNPs. 
E)  cDNA microarrays.

Question 17

The lower limit for detection of deletions in human karyotypes is _________.

Accepted Answer

A)  50kb 
B)  500kb 
C)  5Mb 
D)  50Mb 
E)  500Mb 
A)  50kb 
B)  500kb 
C)  5Mb 
D)  50Mb 
E)  500Mb

Question 18

Unlike pedigree analysis,genome wide association studies do not depend on __________ for gene mapping.

Accepted Answer

A)  reliability of phenotypic information 
B)  availability of human DNA samples 
C)  molecular markers 
D)  knowing family relationships 
E)  control subjects 
A)  reliability of phenotypic information 
B)  availability of human DNA samples 
C)  molecular markers 
D)  knowing family relationships 
E)  control subjects

Question 19

How are the probes for ChIP-on-chip different from probes made for gene expression analysis?

Accepted Answer

A)  Arrays for ChIP-on-chip are made using probes based on intergenic regions. 
B)  Arrays for ChIP-on-chip are made using probes based on cDNAs. 
C)  Arrays for ChIP-on-chip are made using probes based on synthetic oligonucleotides 
D)  Arrays for ChIP-on-chip are made using probes based on rRNAs. 
E)  Arrays for ChIP-on-chip are made using probes based on miRNAs. 
A)  Arrays for ChIP-on-chip are made using probes based on intergenic regions. 
B)  Arrays for ChIP-on-chip are made using probes based on cDNAs. 
C)  Arrays for ChIP-on-chip are made using probes based on synthetic oligonucleotides 
D)  Arrays for ChIP-on-chip are made using probes based on rRNAs. 
E)  Arrays for ChIP-on-chip are made using probes based on miRNAs.

Question 20

Which of the following is required in ChIP-on-chip but not when using a microarray to analyze transcript abundance?

Accepted Answer

A)  fluorescent labels 
B)  an antibody 
C)  PCR 
D)  a microarray 
E)  hybridization 
A)  fluorescent labels 
B)  an antibody 
C)  PCR 
D)  a microarray 
E)  hybridization

Exam 19: The Genetics of Cancer

Which of the following would be most difficult to detect using CGH?

Probes for DNA microarrays are made using

Which of the following is the correct sequence of events in sample preparation for microarray analysis?

Design an experiment to identify genes associated with cancer,using methylome analysis.

Why are genome wide association studies (GWAS)preferable to traditional pedigree analysis for mapping genes associated with complex traits?

Bisulfite sequencing can be included under next-generation sequencing.

Design an experiment to identify genes associated with cancer,using genome wide association studies.

Bisulfite sequencing relies on _______________ to detect ____________.

How are the probes for CGH different from probes made for gene expression analysis?

In genome analysis,presence of the Kozak signal is evidence of

How is CGH used in studying or treating cancer?

Haplotypes are

Antibodies specific to 5-methylcytosine can be used to precipitate DNA prior to array hybridization.

Design an experiment to identify genes associated with cancer,using comparative genome hybridization.

ChIP-seq does not require a microarray for analysis.

Almost all of a person's genetic diversity can be described using

The lower limit for detection of deletions in human karyotypes is _________.

Unlike pedigree analysis,genome wide association studies do not depend on __________ for gene mapping.

How are the probes for ChIP-on-chip different from probes made for gene expression analysis?

Which of the following is required in ChIP-on-chip but not when using a microarray to analyze transcript abundance?

Mendels Principles of Heredity

Extensions to Mendels Laws

The Chromosome Theory of Inheritance

Linkage, Recombination, and the Mapping of Genes on Chromosomes

Dna Structure, Replication, and Recombination

Anatomy and Function of a Gene: Dissection Through Mutation

Gene Expression: the Flow of Information From Dna to Rna to Protein

Digital Analysis of Genomes

Genome Annotation

Analyzing Genomic Variation

The Eukaryotic Chromosome

Chromosomal Rearrangements and Changes in Chromosome Number

Bacterial Genetics

Organellar Inheritance

Gene Regulation in Prokaryotes

Gene Regulation in Eukaryotes

Manipulating the Genomes of Eukaryotes

The Genetic Analysis of Development

Variation and Selection in Populations

Genetics of Complex Traits

Exploring Synthetic Biology: Concepts, Techniques, and Applications

Filters

Bisulfite sequencing relies on ___ to detect .