Question 1

In the Southern blot technique, which of the following is NOT required?

Accepted Answer

A)  electrophoresis to separate fragments 
B)  transfer of DNA to nitrocellulose 
C)  addition of a labeled probe to identify the gene of interest 
D)  restriction enzyme digestion of DNA 
E)  addition of heat-stable DNA polymerase to amplify DNA 
A)  electrophoresis to separate fragments 
B)  transfer of DNA to nitrocellulose 
C)  addition of a labeled probe to identify the gene of interest 
D)  restriction enzyme digestion of DNA 
E)  addition of heat-stable DNA polymerase to amplify DNA E

Question 2

Which of the following is NOT a desired characteristic of DNA vectors used in gene cloning procedures?

Accepted Answer

A)  circular form of DNA or integrates into the host chromosome 
B)  may replicate in several species 
C)  self-replication 
D)  large size 
E)  has a selectable marker 
A)  circular form of DNA or integrates into the host chromosome 
B)  may replicate in several species 
C)  self-replication 
D)  large size 
E)  has a selectable marker D

Question 3

How many pieces will EcoRI produce from the plasmid shown in Figure 9.1?

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  4 
E)  5 
A)  1 
B)  2 
C)  3 
D)  4 
E)  5 B

Question 4

In recombinant DNA technology, a vector is a self-replicating segment of DNA, such as a plasmid or viral genome.

Accepted Answer

A) True 
 B)False

Question 5

An advantage of synthetic DNA over genomic or cDNA is the ability to

Accepted Answer

A)  obtain genes that lack exons. 
B)  make DNA from cellular RNA and the enzyme reverse transcriptase. 
C)  obtain genes that lack introns. 
D)  isolate unknown genes. 
E)  insert desired restriction sites into the DNA sequence. 
A)  obtain genes that lack exons. 
B)  make DNA from cellular RNA and the enzyme reverse transcriptase. 
C)  obtain genes that lack introns. 
D)  isolate unknown genes. 
E)  insert desired restriction sites into the DNA sequence.

Question 6

The term biotechnology refers exclusively to the use of genetically engineered organisms for the production of desired products.

Accepted Answer

A) True 
 B)False

Question 7

The following steps must be performed to make a bacterium produce human protein X. 1- Translation
2- Restriction enzyme
3- Prokaryotic transcription 4-DNA ligase
1- Transformation
2- Eukaryotic transcription 7-Reverse transcription
Which of the following places the steps in the correct order?

Accepted Answer

A)  6, 7, 2, 3, 4, 5, 1 
B)  6, 2, 1, 3, 4, 5, 7 
C)  1, 2, 3, 5, 4, 7, 6 
D)  6, 7, 2, 4, 5, 3, 1 
E)  5, 2, 3, 4, 7, 6, 1 
A)  6, 7, 2, 3, 4, 5, 1 
B)  6, 2, 1, 3, 4, 5, 7 
C)  1, 2, 3, 5, 4, 7, 6 
D)  6, 7, 2, 4, 5, 3, 1 
E)  5, 2, 3, 4, 7, 6, 1

Question 8

Figure 9.2
  
-In Figure 9.2, the enzyme in step 2 is

Accepted Answer

A)  spliceosome. 
B)  reverse transcriptase. 
C)  RNA polymerase. 
D)  DNA polymerase. 
E)  DNA ligase. 
A)  spliceosome. 
B)  reverse transcriptase. 
C)  RNA polymerase. 
D)  DNA polymerase. 
E)  DNA ligase.

Question 9

Figure 9.4   In Figure 9.4, the bacteria transformed with the recombinant plasmid and plated on media containing ampicillin and X-gal will

Accepted Answer

A)  form blue, ampicillin-sensitive colonies. 
B)  form white, ampicillin-resistant colonies. 
C)  form blue, ampicillin-resistant colonies. 
D)  form white, ampicillin-sensitive colonies. 
E)  not grow. 
A)  form blue, ampicillin-sensitive colonies. 
B)  form white, ampicillin-resistant colonies. 
C)  form blue, ampicillin-resistant colonies. 
D)  form white, ampicillin-sensitive colonies. 
E)  not grow.

Question 10

Restriction enzymes are

Accepted Answer

A)  bacterial enzymes that splice DNA. 
B)  animal enzymes that splice RNA. 
C)  bacterial enzymes that destroy phage DNA. 
D)  viral enzymes that destroy host DNA. 
A)  bacterial enzymes that splice DNA. 
B)  animal enzymes that splice RNA. 
C)  bacterial enzymes that destroy phage DNA. 
D)  viral enzymes that destroy host DNA.

Question 11

A population of cells carrying a desired plasmid is called a

Accepted Answer

A)  clone. 
B)  vector. 
C)  Southern blot. 
D)  library. 
E)  PCR. 
A)  clone. 
B)  vector. 
C)  Southern blot. 
D)  library. 
E)  PCR.

Question 12

Which of the following are used to silence specific genes and hold promise for treating cancer or viral diseases, such as hepatitis B?

Accepted Answer

A)  DNA fingerprinting 
B)  RNA interference (RNAi) 
C)  tumor-inducing plasmids (Ti plasmids) 
D)  reverse transcriptase PCR (rtPCR) 
E)  complementary DNA (cDN
A)  
A)  DNA fingerprinting 
B)  RNA interference (RNAi) 
C)  tumor-inducing plasmids (Ti plasmids) 
D)  reverse transcriptase PCR (rtPCR) 
E)  complementary DNA (cDN
A)

Question 13

You want to determine whether a person has a certain mutant gene. The process involves using a primer and a heat-stable DNA polymerase. This process is

Accepted Answer

A)  restriction mapping. 
B)  translation. 
C)  PCR. 
D)  transformation. 
E)  site-directed mutagenesis. 
A)  restriction mapping. 
B)  translation. 
C)  PCR. 
D)  transformation. 
E)  site-directed mutagenesis.

Question 14

Figure 9.5
  
-A source of heat-stable DNA polymerase is

Accepted Answer

A)  Saccharomyces cerevisiae. 
B)  Pseudomonas. 
C)  Thermus aquaticus. 
D)  Bacillus thuringiensis. 
E)  Agrobacterium tumefaciens. 
A)  Saccharomyces cerevisiae. 
B)  Pseudomonas. 
C)  Thermus aquaticus. 
D)  Bacillus thuringiensis. 
E)  Agrobacterium tumefaciens.

Question 15

A restriction fragment is

Accepted Answer

A)  a segment of mRNA. 
B)  a gene. 
C)  cDNA. 
D)  a segment of DNA. 
E)  a segment of tRNA. 
A)  a segment of mRNA. 
B)  a gene. 
C)  cDNA. 
D)  a segment of DNA. 
E)  a segment of tRNA.

Question 16

Gene silencing involves all of the following EXCEPT

Accepted Answer

A)  small interfering RNAs. 
B)  production of double stranded RNAs. 
C)  RNA-induced silencing complex. 
D)  small interfering RNA binding to a gene promoter. 
E)  Dicer. 
A)  small interfering RNAs. 
B)  production of double stranded RNAs. 
C)  RNA-induced silencing complex. 
D)  small interfering RNA binding to a gene promoter. 
E)  Dicer.

Question 17

You have a small gene that you wish replicated by PCR. After 3 replication cycles, how many double-stranded DNA molecules do you have?

Accepted Answer

A)  4 
B)  thousands 
C)  2 
D)  16 
E)  8 
A)  4 
B)  thousands 
C)  2 
D)  16 
E)  8

Question 18

Figure 9.3   The figure at the left in Figure 9.3 shows a gene identified by Southern blotting. What will a Southern blot of the same gene look like after PCR?

Accepted Answer

A)  a 
B)  b 
C)  c 
D)  d 
E)  e 
A)  a 
B)  b 
C)  c 
D)  d 
E)  e

Question 19

PCR can be used to identify an unknown bacterium because

Accepted Answer

A)  all cells have RNA. 
B)  DNA polymerase will replicate DNA. 
C)  all cells have DNA. 
D)  DNA can be electrophoresed. 
E)  the RNA primer is specific. 
A)  all cells have RNA. 
B)  DNA polymerase will replicate DNA. 
C)  all cells have DNA. 
D)  DNA can be electrophoresed. 
E)  the RNA primer is specific.

Question 20

Figure 9.5
  
-The Pap test for cervical cancer involves microscopic examination of cervical cells for cancerous cells. A new, rapid diagnostic test to detect human papilloma virus (HPV) DNA before cancer develops is done without microscopic exam. The steps involved in this FastHPV test are listed below. What is the second step?

Accepted Answer

A)  Add an RNA probe for HPV DNA. 
B)  Add enzyme substrate. 
C)  The order is unimportant. 
D)  Add enzyme-linked antibodies against DNA-RNA. 
E)  Lyse human cells. 
A)  Add an RNA probe for HPV DNA. 
B)  Add enzyme substrate. 
C)  The order is unimportant. 
D)  Add enzyme-linked antibodies against DNA-RNA. 
E)  Lyse human cells.

Exam 9: Biotechnology and Dna Technology

In the Southern blot technique, which of the following is NOT required?

Which of the following is NOT a desired characteristic of DNA vectors used in gene cloning procedures?

How many pieces will EcoRI produce from the plasmid shown in Figure 9.1?

In recombinant DNA technology, a vector is a self-replicating segment of DNA, such as a plasmid or viral genome.

An advantage of synthetic DNA over genomic or cDNA is the ability to

The term biotechnology refers exclusively to the use of genetically engineered organisms for the production of desired products.

The following steps must be performed to make a bacterium produce human protein X. 1- Translation 2- Restriction enzyme 3- Prokaryotic transcription 4-DNA ligase 1- Transformation 2- Eukaryotic transcription 7-Reverse transcription Which of the following places the steps in the correct order?

Figure 9.2 -In Figure 9.2, the enzyme in step 2 is

Figure 9.4 In Figure 9.4, the bacteria transformed with the recombinant plasmid and plated on media containing ampicillin and X-gal will

Restriction enzymes are

A population of cells carrying a desired plasmid is called a

Which of the following are used to silence specific genes and hold promise for treating cancer or viral diseases, such as hepatitis B?

You want to determine whether a person has a certain mutant gene. The process involves using a primer and a heat-stable DNA polymerase. This process is

Figure 9.5 -A source of heat-stable DNA polymerase is

A restriction fragment is

Gene silencing involves all of the following EXCEPT

You have a small gene that you wish replicated by PCR. After 3 replication cycles, how many double-stranded DNA molecules do you have?

Figure 9.3 The figure at the left in Figure 9.3 shows a gene identified by Southern blotting. What will a Southern blot of the same gene look like after PCR?

PCR can be used to identify an unknown bacterium because

The Microbial World and You

Chemical Principles

Observing Microorganisms Through a Microscope

Functional Anatomy of Prokaryotic and Eukaryotic Cells

Microbial Metabolism

Microbial Growth

The Control of Microbial Growth

Microbial Genetics

Classification of Microorganisms

The Prokaryotes: Domains Bacteria and Archaea

The Eukaryotes: Fungi, Algae, Protozoa, and Helminths

Viruses, Viroids, and Prions

Principles of Disease and Epidemiology

Microbial Mechanisms of Pathogenicity

Adaptive Immunity: Specific Defenses of the Host

Practical Applications of Immunology

Disorders Associated With the Immune System

Antimicrobial Drugs

Microbial Diseases of the Skin and Eyes

Microbial Diseases of the Nervous System

Microbial Diseases of the Cardiovascular and Lymphatic Systems

Microbial Diseases of the Respiratory System

Microbial Diseases of the Digestive System

Microbial Disease of the Urinary and Reproductive Systems

Environmental Microbiology

Applied and Industrial Microbiology

Innate Immunity: Nonspecific Defenses of the Host

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