Question 1

The random shotgun method is used in

Accepted Answer

A)  amplification of unknown DNA. 
B)  genome sequencing. 
C)  transforming plant cells with recombinant DNA. 
D)  forensic microbiology. 
E)  RFLP analysis. 
A)  amplification of unknown DNA. 
B)  genome sequencing. 
C)  transforming plant cells with recombinant DNA. 
D)  forensic microbiology. 
E)  RFLP analysis.

Question 2

Which of the following techniques is NOT used to introduce recombinant DNA into plants?

Accepted Answer

A)  microinjection 
B)  Ti plasmids and Agrobacterium 
C)  gene guns 
D)  electroporation 
E)  protoplast fusion 
A)  microinjection 
B)  Ti plasmids and Agrobacterium 
C)  gene guns 
D)  electroporation 
E)  protoplast fusion

Question 3

A colleague has used computer modeling to design an improved enzyme. To produce this enzyme, the next step is to

Accepted Answer

A)  look for a bacterium that makes the improved enzyme. 
B)  use siRNA to produce the enzyme. 
C)  mutate bacteria until one makes the improved enzyme. 
D)  determine the nucleotide sequence for the improved enzyme. 
E)  synthesize the gene for the improved enzyme. 
A)  look for a bacterium that makes the improved enzyme. 
B)  use siRNA to produce the enzyme. 
C)  mutate bacteria until one makes the improved enzyme. 
D)  determine the nucleotide sequence for the improved enzyme. 
E)  synthesize the gene for the improved enzyme.

Question 4

The following are steps used to make DNA fingerprints. What is the third step?

Accepted Answer

A)  Lyse cells. 
B)  Add stain. 
C)  Digest with a restriction enzyme. 
D)  Perform electrophoresis. 
E)  Collect DNA. 
A)  Lyse cells. 
B)  Add stain. 
C)  Digest with a restriction enzyme. 
D)  Perform electrophoresis. 
E)  Collect DNA.

Question 5

Which of the following is an advantage of using E. coli to make a human gene product?

Accepted Answer

A)  It cannot process introns. 
B)  It does not secrete most proteins. 
C)  Endotoxin may be in the product. 
D)  Its genes are well known. 
E)  Endotoxin may be in the product and it does not secrete most proteins. 
A)  It cannot process introns. 
B)  It does not secrete most proteins. 
C)  Endotoxin may be in the product. 
D)  Its genes are well known. 
E)  Endotoxin may be in the product and it does not secrete most proteins.

Question 6

If you have inserted a gene in the Ti plasmid, the next step in genetic engineering is

Accepted Answer

A)  transformation of an animal cell. 
B)  inserting the Ti plasmid into a plant cell. 
C)  inserting the Ti plasmid into Agrobacterium. 
D)  splicing T DNA into a plasmid. 
E)  transformation of E. coli with Ti plasmid. 
A)  transformation of an animal cell. 
B)  inserting the Ti plasmid into a plant cell. 
C)  inserting the Ti plasmid into Agrobacterium. 
D)  splicing T DNA into a plasmid. 
E)  transformation of E. coli with Ti plasmid.

Question 7

The use of ʺsuicideʺ genes in genetically modified organisms is designed to

Accepted Answer

A)  provide a means to eliminate non-modified organisms. 
B)  prevent the growth of the modified organisms in the environment. 
C)  provide for resistance of the modified organisms to pesticides. 
D)  delete genes necessary for modified organismʹs growth. 
E)  kill the modified organisms before they are released in the environment. 
A)  provide a means to eliminate non-modified organisms. 
B)  prevent the growth of the modified organisms in the environment. 
C)  provide for resistance of the modified organisms to pesticides. 
D)  delete genes necessary for modified organismʹs growth. 
E)  kill the modified organisms before they are released in the environment.

Question 8

Figure 9.1
  
-In Figure 9.1, after digestion with the appropriate restriction enzyme, what is the smallest piece containing the entire ampicillin-resistance (amp) gene?

Accepted Answer

A)  0.17 kbp 
B)  0.25 kbp 
C)  1.50 kbp 
D)  3.00 kbp 
E)  1.08 kbp 
A)  0.17 kbp 
B)  0.25 kbp 
C)  1.50 kbp 
D)  3.00 kbp 
E)  1.08 kbp

Question 9

Figure 9.5
  
-In Figure 9.5, the gene that allows the plasmid to be self-replicating is

Accepted Answer

A)  lacZ. 
B)  ori. 
C)  HindIII. 
D)  ampR. 
E)  EcoRI. 
A)  lacZ. 
B)  ori. 
C)  HindIII. 
D)  ampR. 
E)  EcoRI.

Question 10

Which of the following processes is NOT involved in making cDNA?

Accepted Answer

A)  reverse transcription 
B)  translation 
C)  RNA processing to remove introns 
D)  transcription 
A)  reverse transcription 
B)  translation 
C)  RNA processing to remove introns 
D)  transcription

Question 11

Which of the following are used by the Centers for Disease Control and Prevention to track outbreaks of foodborne disease?

Accepted Answer

A)  reverse-transcriptase PCR (rtPCR) 
B)  DNA fingerprints 
C)  restriction fragment length polymorphisms 
D)  DNA fingerprints, restriction fragment length polymorphisms, and reverse -transcriptase PCR(rtPCR) 
E)  DNA fingerprints and restriction fragment length polymorphisms 
A)  reverse-transcriptase PCR (rtPCR) 
B)  DNA fingerprints 
C)  restriction fragment length polymorphisms 
D)  DNA fingerprints, restriction fragment length polymorphisms, and reverse -transcriptase PCR(rtPCR) 
E)  DNA fingerprints and restriction fragment length polymorphisms

Question 12

Bioinformatics is the use of computer technology to compare and analyze genome sequence.

Accepted Answer

A) True 
 B)False

Question 13

Self-replicating DNA used to transmit a gene from one organism to another is a

Accepted Answer

A)  vector. 
B)  clone. 
C)  Southern blot. 
D)  library. 
E)  PCR. 
A)  vector. 
B)  clone. 
C)  Southern blot. 
D)  library. 
E)  PCR.

Exam 9: Biotechnology and Dna Technology

The random shotgun method is used in

Which of the following techniques is NOT used to introduce recombinant DNA into plants?

A colleague has used computer modeling to design an improved enzyme. To produce this enzyme, the next step is to

The following are steps used to make DNA fingerprints. What is the third step?

Which of the following is an advantage of using E. coli to make a human gene product?

If you have inserted a gene in the Ti plasmid, the next step in genetic engineering is

The use of ʺsuicideʺ genes in genetically modified organisms is designed to

Figure 9.1 -In Figure 9.1, after digestion with the appropriate restriction enzyme, what is the smallest piece containing the entire ampicillin-resistance (amp) gene?

Figure 9.5 -In Figure 9.5, the gene that allows the plasmid to be self-replicating is

Which of the following processes is NOT involved in making cDNA?

Which of the following are used by the Centers for Disease Control and Prevention to track outbreaks of foodborne disease?

Bioinformatics is the use of computer technology to compare and analyze genome sequence.

Self-replicating DNA used to transmit a gene from one organism to another is a

The Microbial World and You

Chemical Principles

Observing Microorganisms Through a Microscope

Functional Anatomy of Prokaryotic and Eukaryotic Cells

Microbial Metabolism

Microbial Growth

The Control of Microbial Growth

Microbial Genetics

Classification of Microorganisms

The Prokaryotes: Domains Bacteria and Archaea

The Eukaryotes: Fungi, Algae, Protozoa, and Helminths

Viruses, Viroids, and Prions

Principles of Disease and Epidemiology

Microbial Mechanisms of Pathogenicity

Adaptive Immunity: Specific Defenses of the Host

Practical Applications of Immunology

Disorders Associated With the Immune System

Antimicrobial Drugs

Microbial Diseases of the Skin and Eyes

Microbial Diseases of the Nervous System

Microbial Diseases of the Cardiovascular and Lymphatic Systems

Microbial Diseases of the Respiratory System

Microbial Diseases of the Digestive System

Microbial Disease of the Urinary and Reproductive Systems

Environmental Microbiology

Applied and Industrial Microbiology

Innate Immunity: Nonspecific Defenses of the Host

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