Question 1

Which of the following places the steps in the correct sequence? 1- Staining
2- Making a smear 3-Fixing

Accepted Answer

A)  3-2-1 
B)  1-2-3 
C)  1-3-2 
D)  2-3-1 
E)  The order is unimportant. 
A)  3-2-1 
B)  1-2-3 
C)  1-3-2 
D)  2-3-1 
E)  The order is unimportant.

Question 2

The limit of resolution of the compound microscope illuminated with visible light is approximately
0.2 μm.

Accepted Answer

A) True 
 B)False

Question 3

Which microscope is best used for observing the surfaces of intact cells and viruses?

Accepted Answer

A)  darkfield microscope 
B)  phase-contrast microscope 
C)  scanning electron microscope 
D)  brightfield microscope 
E)  fluorescence microscope 
A)  darkfield microscope 
B)  phase-contrast microscope 
C)  scanning electron microscope 
D)  brightfield microscope 
E)  fluorescence microscope

Question 4

You are studying a cell structure that is approximately 100 nm in size. Which of the following provides the greatest magnification you can use to see this structure?

Accepted Answer

A)  brightfield microscope 
B)  phase-contrast microscope 
C)  scanning electron microscope 
D)  darkfield microscope 
E)  transmission electron microscope 
A)  brightfield microscope 
B)  phase-contrast microscope 
C)  scanning electron microscope 
D)  darkfield microscope 
E)  transmission electron microscope

Question 5

Which microscope is used to see intracellular detail in a living cell?

Accepted Answer

A)  fluorescence microscope 
B)  transmission electron microscope 
C)  brightfield microscope 
D)  atomic force microscope 
E)  two-photon microscope 
A)  fluorescence microscope 
B)  transmission electron microscope 
C)  brightfield microscope 
D)  atomic force microscope 
E)  two-photon microscope

Question 6

Which microscope is used to observe viruses and the internal structure of thinly sectioned cells?

Accepted Answer

A)  brightfield microscope 
B)  scanning electron microscope 
C)  darkfield microscope 
D)  fluorescence microscope 
E)  transmission electron microscope 
A)  brightfield microscope 
B)  scanning electron microscope 
C)  darkfield microscope 
D)  fluorescence microscope 
E)  transmission electron microscope

Question 7

Which microscope uses two beams of light to produce a three-dimensional color image?

Accepted Answer

A)  phase-contrast microscope 
B)  DIC microscope 
C)  electron microscope 
D)  fluorescence microscope 
E)  darkfield microscope 
A)  phase-contrast microscope 
B)  DIC microscope 
C)  electron microscope 
D)  fluorescence microscope 
E)  darkfield microscope

Question 8

What structure does light pass through after leaving the condenser in a compound light microscope?

Accepted Answer

A)  illuminator 
B)  objective lens 
C)  specimen 
D)  ocular lens 
A)  illuminator 
B)  objective lens 
C)  specimen 
D)  ocular lens

Question 9

The greater resolution of the electron microscope compared to the compound microscope is due to the longer wavelengths of the electrons used to examine specimens.

Accepted Answer

A) True 
 B)False

Question 10

Which microscope is used to see internal structures of cells in a natural state?

Accepted Answer

A)  fluorescence microscope 
B)  phase-contrast microscope 
C)  compound light microscope 
D)  electron microscope 
E)  darkfield microscope 
A)  fluorescence microscope 
B)  phase-contrast microscope 
C)  compound light microscope 
D)  electron microscope 
E)  darkfield microscope

Question 11

Which of the following pairs is mismatched?

Accepted Answer

A)  confocal microscope ‐ produces a three-dimensional image 
B)  scanning electron microscope ‐ produces a three-dimensional image 
C)  fluorescence microscope ‐ uses a fluorescent light 
D)  darkfield microscope ‐ uses visible light 
E)  scanning tunneling microscope ‐ allows visualization of atoms 
A)  confocal microscope ‐ produces a three-dimensional image 
B)  scanning electron microscope ‐ produces a three-dimensional image 
C)  fluorescence microscope ‐ uses a fluorescent light 
D)  darkfield microscope ‐ uses visible light 
E)  scanning tunneling microscope ‐ allows visualization of atoms

Question 12

Which microscope is used to observe a specimen that emits light when illuminated with an ultraviolet light?

Accepted Answer

A)  darkfield microscope 
B)  electron microscope 
C)  phase-contrast microscope 
D)  compound light microscope 
E)  fluorescence microscope 
A)  darkfield microscope 
B)  electron microscope 
C)  phase-contrast microscope 
D)  compound light microscope 
E)  fluorescence microscope

Question 13

The negative stain is used to

Accepted Answer

A)  determine cell size. 
B)  determine Gram reaction. 
C)  visualize endospores. 
D)  determine flagella arrangement. 
E)  visualize capsules. 
A)  determine cell size. 
B)  determine Gram reaction. 
C)  visualize endospores. 
D)  determine flagella arrangement. 
E)  visualize capsules.

Question 14

Which microscope can be used to visualize DNA or botulinum toxin?

Accepted Answer

A)  compound light microscope 
B)  phase-contrast microscope 
C)  scanning tunneling microscope 
D)  scanning electron microscope 
E)  confocal microscope 
A)  compound light microscope 
B)  phase-contrast microscope 
C)  scanning tunneling microscope 
D)  scanning electron microscope 
E)  confocal microscope

Question 15

Both phase-contrast microscopy and differential interference contrast microscopy are used to view the internal structures of cells without staining.

Accepted Answer

A) True 
 B)False

Question 16

Scanned probe microscopy is used to examine fine detail of molecular complexes, such as blood clots, or molecules, such as DNA.

Accepted Answer

A) True 
 B)False

Question 17

A student is looking at a bacterial specimen using the oil immersion lens, but has forgotten to put immersion oil on the slide. The specimen will appear

Accepted Answer

A)  smaller than it would if immersion oil was used. 
B)  somewhat fuzzy and have poor resolution. 
C)  the same as it would if the immersion oil was used. 
D)  larger than it would if immersion oil was used. 
E)  to have no color. 
A)  smaller than it would if immersion oil was used. 
B)  somewhat fuzzy and have poor resolution. 
C)  the same as it would if the immersion oil was used. 
D)  larger than it would if immersion oil was used. 
E)  to have no color.

Question 18

Which of the following pairs is mismatched?

Accepted Answer

A)  methylene blue ‐ simple stain 
B)  crystal violet ‐ simple stain 
C)  nigrosin ‐ negative stain 
D)  basic dye ‐ negative stain 
E)  acidic dye ‐ capsule stain 
A)  methylene blue ‐ simple stain 
B)  crystal violet ‐ simple stain 
C)  nigrosin ‐ negative stain 
D)  basic dye ‐ negative stain 
E)  acidic dye ‐ capsule stain

Question 19

You are performing a Gram stain on gram-positive bacteria and you stop after the addition of the first dye. What is the appearance of the bacteria at this point?

Accepted Answer

A)  purple 
B)  red 
C)  colorless 
D)  brown 
A)  purple 
B)  red 
C)  colorless 
D)  brown

Question 20

Assume you stain Bacillus by applying malachite green with heat and then counterstaining with safranin. Through the microscope, the green structures are

Accepted Answer

A)  capsules. 
B)  flagella. 
C)  endospores. 
D)  cell walls. 
E)  The answer cannot be determined. 
A)  capsules. 
B)  flagella. 
C)  endospores. 
D)  cell walls. 
E)  The answer cannot be determined.

Exam 3: Observing Microorganisms Through a Microscope

Which of the following places the steps in the correct sequence? 1- Staining 2- Making a smear 3-Fixing

The limit of resolution of the compound microscope illuminated with visible light is approximately 0.2 μm.

Which microscope is best used for observing the surfaces of intact cells and viruses?

You are studying a cell structure that is approximately 100 nm in size. Which of the following provides the greatest magnification you can use to see this structure?

Which microscope is used to see intracellular detail in a living cell?

Which microscope is used to observe viruses and the internal structure of thinly sectioned cells?

Which microscope uses two beams of light to produce a three-dimensional color image?

What structure does light pass through after leaving the condenser in a compound light microscope?

The greater resolution of the electron microscope compared to the compound microscope is due to the longer wavelengths of the electrons used to examine specimens.

Which microscope is used to see internal structures of cells in a natural state?

Which of the following pairs is mismatched?

Which microscope is used to observe a specimen that emits light when illuminated with an ultraviolet light?

The negative stain is used to

Which microscope can be used to visualize DNA or botulinum toxin?

Both phase-contrast microscopy and differential interference contrast microscopy are used to view the internal structures of cells without staining.

Scanned probe microscopy is used to examine fine detail of molecular complexes, such as blood clots, or molecules, such as DNA.

A student is looking at a bacterial specimen using the oil immersion lens, but has forgotten to put immersion oil on the slide. The specimen will appear

Which of the following pairs is mismatched?

You are performing a Gram stain on gram-positive bacteria and you stop after the addition of the first dye. What is the appearance of the bacteria at this point?

Assume you stain Bacillus by applying malachite green with heat and then counterstaining with safranin. Through the microscope, the green structures are

The Microbial World and You

Chemical Principles

Functional Anatomy of Prokaryotic and Eukaryotic Cells

Microbial Metabolism

Microbial Growth

The Control of Microbial Growth

Microbial Genetics

Biotechnology and Dna Technology

Classification of Microorganisms

The Prokaryotes: Domains Bacteria and Archaea

The Eukaryotes: Fungi, Algae, Protozoa, and Helminths

Viruses, Viroids, and Prions

Principles of Disease and Epidemiology

Microbial Mechanisms of Pathogenicity

Adaptive Immunity: Specific Defenses of the Host

Practical Applications of Immunology

Disorders Associated With the Immune System

Antimicrobial Drugs

Microbial Diseases of the Skin and Eyes

Microbial Diseases of the Nervous System

Microbial Diseases of the Cardiovascular and Lymphatic Systems

Microbial Diseases of the Respiratory System

Microbial Diseases of the Digestive System

Microbial Disease of the Urinary and Reproductive Systems

Environmental Microbiology

Applied and Industrial Microbiology

Innate Immunity: Nonspecific Defenses of the Host

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