Question 1

The counterstain used in the Gram stain is a basic dye.

Accepted Answer

A) True 
 B)False

Question 2

The purpose of a mordant in the Gram stain is to

Accepted Answer

A)  make the bacterial cells larger. 
B)  make the flagella visible. 
C)  remove the simple stain. 
D)  prevent the crystal violet from leaving the cells. 
E)  make gram-negative cells visible. 
A)  make the bacterial cells larger. 
B)  make the flagella visible. 
C)  remove the simple stain. 
D)  prevent the crystal violet from leaving the cells. 
E)  make gram-negative cells visible.

Question 3

In a completed Gram stain, gram-negative bacteria are colorless.

Accepted Answer

A) True 
 B)False

Question 4

Which microscope is most useful for visualizing a biofilm?

Accepted Answer

A)  phase-contrast microscope 
B)  scanning acoustic microscope 
C)  transmission electron microscope 
D)  fluorescence microscope 
E)  compound light microscope 
A)  phase-contrast microscope 
B)  scanning acoustic microscope 
C)  transmission electron microscope 
D)  fluorescence microscope 
E)  compound light microscope

Question 5

Which of the following places the steps of the Gram stain in the correct order? 1-Alcohol-acetone 2-Crystal violet
3-Safranin 4-Iodine

Accepted Answer

A)  1-3-2-4 
B)  1-2-3-4 
C)  2-1-4-3 
D)  2-4-1-3 
E)  4-3-2-1 
A)  1-3-2-4 
B)  1-2-3-4 
C)  2-1-4-3 
D)  2-4-1-3 
E)  4-3-2-1

Question 6

The capsules and flagella of bacteria can be observed in gram -stained smears.

Accepted Answer

A) True 
 B)False

Question 7

You find colorless areas in cells in a Gram-stained smear. What should you apply next?

Accepted Answer

A)  an acid-fast stain 
B)  a flagella stain 
C)  an endospore stain 
D)  a capsule stain 
E)  a simple stain 
A)  an acid-fast stain 
B)  a flagella stain 
C)  an endospore stain 
D)  a capsule stain 
E)  a simple stain

Question 8

Which type of stain is most useful in helping clinicians to decide which antibiotic to prescribe for a bacterial infection?

Accepted Answer

A)  negative stain 
B)  Gram stain 
C)  endospore stain 
D)  simple stain 
E)  flagella stain 
A)  negative stain 
B)  Gram stain 
C)  endospore stain 
D)  simple stain 
E)  flagella stain

Question 9

In microscopy, the term resolution

Accepted Answer

A)  refers to the ability to distinguish fine structure and detail in a specimen. 
B)  refers to magnification when using the electron microscope. 
C)  is improved when longer wavelengths of light are employed. 
D)  is only observed in stained specimens. 
E)  is the same as the total magnification of a specimen. 
A)  refers to the ability to distinguish fine structure and detail in a specimen. 
B)  refers to magnification when using the electron microscope. 
C)  is improved when longer wavelengths of light are employed. 
D)  is only observed in stained specimens. 
E)  is the same as the total magnification of a specimen.

Question 10

Which microscope uses visible light?

Accepted Answer

A)  confocal microscope 
B)  differential interference contrast microscope 
C)  scanning acoustic microscope 
D)  scanning electron microscope 
E)  fluorescence microscope 
A)  confocal microscope 
B)  differential interference contrast microscope 
C)  scanning acoustic microscope 
D)  scanning electron microscope 
E)  fluorescence microscope

Question 11

If acid-fast bacteria are stained with the Gram stain, they will stain gram-negative.

Accepted Answer

A) True 
 B)False

Question 12

In using this microscope, the observer does NOT look directly at an image through a lens.

Accepted Answer

A)  fluorescence microscope 
B)  phase-contrast microscope 
C)  compound light microscope 
D)  darkfield microscope 
E)  electron microscope 
A)  fluorescence microscope 
B)  phase-contrast microscope 
C)  compound light microscope 
D)  darkfield microscope 
E)  electron microscope

Question 13

What is the total magnification of a specimen viewed with a 10x ocular lens and a 45x objective lens?

Accepted Answer

A)  4.5x 
B)  45x 
C)  100x 
D)  10x 
E)  450x 
A)  4.5x 
B)  45x 
C)  100x 
D)  10x 
E)  450x

Question 14

The purpose of the ocular lens is to

Accepted Answer

A)  magnify the image from the objective lens. 
B)  decrease the refractive index. 
C)  decrease the light. 
D)  increase the light. 
E)  improve resolution. 
A)  magnify the image from the objective lens. 
B)  decrease the refractive index. 
C)  decrease the light. 
D)  increase the light. 
E)  improve resolution.

Question 15

This microscope produces an image of a light cell against a dark background; internal structures are NOT visible.

Accepted Answer

A)  phase-contrast microscope 
B)  fluorescence microscope 
C)  darkfield microscope 
D)  electron microscope 
E)  compound light microscope 
A)  phase-contrast microscope 
B)  fluorescence microscope 
C)  darkfield microscope 
D)  electron microscope 
E)  compound light microscope

Exam 3: Observing Microorganisms Through a Microscope

The counterstain used in the Gram stain is a basic dye.

The purpose of a mordant in the Gram stain is to

In a completed Gram stain, gram-negative bacteria are colorless.

Which microscope is most useful for visualizing a biofilm?

Which of the following places the steps of the Gram stain in the correct order? 1-Alcohol-acetone 2-Crystal violet 3-Safranin 4-Iodine

The capsules and flagella of bacteria can be observed in gram -stained smears.

You find colorless areas in cells in a Gram-stained smear. What should you apply next?

Which type of stain is most useful in helping clinicians to decide which antibiotic to prescribe for a bacterial infection?

In microscopy, the term resolution

Which microscope uses visible light?

If acid-fast bacteria are stained with the Gram stain, they will stain gram-negative.

In using this microscope, the observer does NOT look directly at an image through a lens.

What is the total magnification of a specimen viewed with a 10x ocular lens and a 45x objective lens?

The purpose of the ocular lens is to

This microscope produces an image of a light cell against a dark background; internal structures are NOT visible.

The Microbial World and You

Chemical Principles

Functional Anatomy of Prokaryotic and Eukaryotic Cells

Microbial Metabolism

Microbial Growth

The Control of Microbial Growth

Microbial Genetics

Biotechnology and Dna Technology

Classification of Microorganisms

The Prokaryotes: Domains Bacteria and Archaea

The Eukaryotes: Fungi, Algae, Protozoa, and Helminths

Viruses, Viroids, and Prions

Principles of Disease and Epidemiology

Microbial Mechanisms of Pathogenicity

Adaptive Immunity: Specific Defenses of the Host

Practical Applications of Immunology

Disorders Associated With the Immune System

Antimicrobial Drugs

Microbial Diseases of the Skin and Eyes

Microbial Diseases of the Nervous System

Microbial Diseases of the Cardiovascular and Lymphatic Systems

Microbial Diseases of the Respiratory System

Microbial Diseases of the Digestive System

Microbial Disease of the Urinary and Reproductive Systems

Environmental Microbiology

Applied and Industrial Microbiology

Innate Immunity: Nonspecific Defenses of the Host

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