Question 1

Bone marrow transplants typically use what type of cells?

Accepted Answer

A)  Embryonic stem cells 
B)  Embryonic germ cells 
C)  Embryonic carcinoma cells 
D)  Hematopoietic stem cells 
E)  None of the answers are correct 
A)  Embryonic stem cells 
B)  Embryonic germ cells 
C)  Embryonic carcinoma cells 
D)  Hematopoietic stem cells 
E)  None of the answers are correct D

Question 2

Using the NeoR/Tk system of introducing gene replacements into mice (as described in the text), which of the following gene combinations would identify those mouse cells that were the result of homologous recombination?

Accepted Answer

A)  NeoR only 
B)  Tk only 
C)  NeoR and Tk 
D)  Neither NeoR or Tk 
A)  NeoR only 
B)  Tk only 
C)  NeoR and Tk 
D)  Neither NeoR or Tk A

Question 3

Which of the following can be used as a vector for gene therapy?

Accepted Answer

A)  Liposomes 
B)  Parvoviruses 
C)  Adenoviruses 
D)  Retroviruses 
E)  All of the answers are correct 
A)  Liposomes 
B)  Parvoviruses 
C)  Adenoviruses 
D)  Retroviruses 
E)  All of the answers are correct D

Question 4

Cells that have undergone homologous recombination for gene replacement would have which of the following gene combinations?

Accepted Answer

A)  Only the cloned gene 
B)  Only the original gene 
C)  Both the cloned and original gene 
D)  Neither the cloned or original gene 
A)  Only the cloned gene 
B)  Only the original gene 
C)  Both the cloned and original gene 
D)  Neither the cloned or original gene

Question 5

An individual who contains cells that are mutated is called a chimera.

Accepted Answer

A) True 
 B)False

Question 6

A transgenic organism has recombinant DNA integrated into its genome.

Accepted Answer

A) True 
 B)False

Question 7

An organism that can be regenerated by somatic cells is called multipotent.

Accepted Answer

A) True 
 B)False

Question 8

Which of the following is an example of a pluripotent cell?

Accepted Answer

A)  embryonic stem cells 
B)  red blood cells 
C)  fetal heart cells 
D)  umbilical cord blood 
E)  nerve cells 
A)  embryonic stem cells 
B)  red blood cells 
C)  fetal heart cells 
D)  umbilical cord blood 
E)  nerve cells

Question 9

The process by which an electrical charge is used to introduce DNA into a cell to produce a transgenic
organism is called electroporation.

Accepted Answer

A) True 
 B)False

Question 10

The primary interest of genetic research into stem cells is the desire to be able to clone a human
being.

Accepted Answer

A) True 
 B)False

Question 11

The reduction of pollutants in the environment by microorganisms is called biodegradation.

Accepted Answer

A) True 
 B)False

Question 12

Why was the addition of methionine necessary during the production of somatostatin by recombinant bacteria?

Accepted Answer

A)  So that it was compatible with the restriction enzyme 
B)  As a selectable marker 
C)  To allow the somatostatin to link to a bacterial protein 
D)  To increase the length of the peptide to make it more commercial 
E)  None of the answers are correct 
A)  So that it was compatible with the restriction enzyme 
B)  As a selectable marker 
C)  To allow the somatostatin to link to a bacterial protein 
D)  To increase the length of the peptide to make it more commercial 
E)  None of the answers are correct

Question 13

Unipotent cells may differentiate into all other cell types of the body.

Accepted Answer

A) True 
 B)False

Question 14

The first hormone to be made by recombinant bacteria was .

Accepted Answer

A)  Insulin 
B)  Glucagons 
C)  Somatostatin 
D)  Testosterone 
E)  None of the answers are correct 
A)  Insulin 
B)  Glucagons 
C)  Somatostatin 
D)  Testosterone 
E)  None of the answers are correct

Question 15

Lipid vesicles that are used to introduce DNA into a cell for gene therapy are called liposomes.

Accepted Answer

A) True 
 B)False

Question 16

Which of the following cells is not pluripotent?

Accepted Answer

A)  ES cells 
B)  EG cells 
C)  EC cells 
D)  All of the answers are pluripotent 
E)  None of the answers are pluripotent 
A)  ES cells 
B)  EG cells 
C)  EC cells 
D)  All of the answers are pluripotent 
E)  None of the answers are pluripotent

Question 17

In gene addition, homologous recombination is used to remove the original gene and replace it with the cloned gene.

Accepted Answer

A) True 
 B)False

Question 18

Identical twins are an example of gene cloning.

Accepted Answer

A) True 
 B)False

Question 19

The use of microorganisms or their products to alleviate plant disease is called biological control.

Accepted Answer

A) True 
 B)False

Question 20

Bioremediation has been used to treat which of the following?

Accepted Answer

A)  Oil spills 
B)  Sewage 
C)  Pesticides 
D)  Heavy metals 
E)  All of the answers have been successfully remediated 
A)  Oil spills 
B)  Sewage 
C)  Pesticides 
D)  Heavy metals 
E)  All of the answers have been successfully remediated

Exam 19: Biotechnology

Bone marrow transplants typically use what type of cells?

Using the NeoR/Tk system of introducing gene replacements into mice (as described in the text), which of the following gene combinations would identify those mouse cells that were the result of homologous recombination?

Which of the following can be used as a vector for gene therapy?

Cells that have undergone homologous recombination for gene replacement would have which of the following gene combinations?

An individual who contains cells that are mutated is called a chimera.

A transgenic organism has recombinant DNA integrated into its genome.

An organism that can be regenerated by somatic cells is called multipotent.

Which of the following is an example of a pluripotent cell?

The process by which an electrical charge is used to introduce DNA into a cell to produce a transgenic organism is called electroporation.

The primary interest of genetic research into stem cells is the desire to be able to clone a human being.

The reduction of pollutants in the environment by microorganisms is called biodegradation.

Why was the addition of methionine necessary during the production of somatostatin by recombinant bacteria?

Unipotent cells may differentiate into all other cell types of the body.

The first hormone to be made by recombinant bacteria was .

Lipid vesicles that are used to introduce DNA into a cell for gene therapy are called liposomes.

Which of the following cells is not pluripotent?

In gene addition, homologous recombination is used to remove the original gene and replace it with the cloned gene.

Identical twins are an example of gene cloning.

The use of microorganisms or their products to alleviate plant disease is called biological control.

Bioremediation has been used to treat which of the following?

Overview of Genetics

Mendelian Inheritance

Reproduction and Chromosome Transmission

Extensions of Mendelian Inheritance

Non-Mendelian Inheritance

Genetic Linkage and Mapping in Eukaryotes

Genetic Transfer and Mapping in Bacteria and

Variation in Chromosome Structure and Number

Molecular Structure of Dna and Rna

Chromosome Organization and Molecular Structure

Dna Replication

Gene Transcription and Rna Modification

Translation of Mrna

Gene Regulation in Bacteria and Bacteriophages

Gene Regulation in Eukaryotes

Gene Mutation and Dna Repair

Recombination and Transposition at the Molecular

Recombinant Dna Technology

Genomics I: Analysis of Dna

Genomics II: Functional Genomics, Proteomics, and

Medical Genetics and Cancer

Developmental Genetics

Population Genetics

Quantitative Genetics

Evolutionary Genetics

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