Question 1

Activator proteins bind to silencer sequences and repressor proteins bind to enhancer sequences.

Accepted Answer

A) True 
 B)False  False

Question 2

A heterodimer occurs when two identical transcription factors interact on a sequence of DNA.
FALSE

Accepted Answer

A) True 
 B)False  False

Question 3

Which of the following is incorrect regarding the glucocorticoid hormomes?

Accepted Answer

A)  They interact with receptors located in the plasma membrane of the cell 
B)  After interacting with the receptor, they release HSP90 
C)  The receptors form a homodimer that travels to the nucleus 
D)  The homodimer interacts with GRE, activating transcription 
A)  They interact with receptors located in the plasma membrane of the cell 
B)  After interacting with the receptor, they release HSP90 
C)  The receptors form a homodimer that travels to the nucleus 
D)  The homodimer interacts with GRE, activating transcription A

Question 4

In which of the following scenarios would gene expression be the lowest?

Accepted Answer

A)  The CpG island upstream of the gene is unmethylated 
B)  Injecting antisense RNA corresponding to the mRNA of the gene 
C)  Deletion of a sequence upstream of the gene known to be a silencer 
D)  Injecting double-stranded RNA corresponding to the mRNA of the gene 
A)  The CpG island upstream of the gene is unmethylated 
B)  Injecting antisense RNA corresponding to the mRNA of the gene 
C)  Deletion of a sequence upstream of the gene known to be a silencer 
D)  Injecting double-stranded RNA corresponding to the mRNA of the gene

Question 5

eIF2a is phosphorylated in order to inhibit transcription. What is occurring at the molecular level?

Accepted Answer

A)  Phosphorylated eIF2a binds to tRNAs, preventing them from attaching its amino acid 
B)  Phosphorylated eIF2a binds to eIF2B, inhibiting its activity 
C)  Phosphorylated eIF2a binds to the mRNA, blocking the Shine-Delgarno sequence and preventing translational initiation 
D)  All of the answers are correct 
A)  Phosphorylated eIF2a binds to tRNAs, preventing them from attaching its amino acid 
B)  Phosphorylated eIF2a binds to eIF2B, inhibiting its activity 
C)  Phosphorylated eIF2a binds to the mRNA, blocking the Shine-Delgarno sequence and preventing translational initiation 
D)  All of the answers are correct

Question 6

What structural motifs promote dimerization?

Accepted Answer

A)  Zinc finger 
B)  Leucine zipper 
C)  Helix-turn-helix 
D)  Helix-loop-helix 
A)  Zinc finger 
B)  Leucine zipper 
C)  Helix-turn-helix 
D)  Helix-loop-helix

Question 7

Which mechanisms are used by miRNAs to regulate gene expression?

Accepted Answer

A)  Targeted degradation of mRNAs 
B)  Targeted inhibition of mRNA translation 
C)  Both A and B 
D)  Neither A nor B 
A)  Targeted degradation of mRNAs 
B)  Targeted inhibition of mRNA translation 
C)  Both A and B 
D)  Neither A nor B

Question 8

Genomic imprinting is a result of .

Accepted Answer

A)  Nucleosome location 
B)  Histone activation 
C)  DNA methylation 
D)  Serine to leucine changes in the genetic code 
A)  Nucleosome location 
B)  Histone activation 
C)  DNA methylation 
D)  Serine to leucine changes in the genetic code

Question 9

Based on the following mature mRNAs, what exons are constitutive? I. 1-2-3-4-7-8-10 II. 2-4-5-6-7-9 III. 1-4-6-7-8 IV. 1-2-4-7-10

Accepted Answer

A)  1 and 2 
B)  1 and 4 
C)  2 and 7 
D)  4 and 7 
A)  1 and 2 
B)  1 and 4 
C)  2 and 7 
D)  4 and 7

Question 10

Explain why yeast genes with a single intron have essentially no alternative splicing.

Accepted Answer

A)  Yeast genes never have alternative splicing. 
B)  Removal of a single intron leads to splicing of the poly-A tail which prevents further splicing. 
C)  Methylation of the single intron prevents further splicing. 
D)  Removal of a single intron only leads to one possible outcome for spliced mRNA. 
E)  None of the above. 
A)  Yeast genes never have alternative splicing. 
B)  Removal of a single intron leads to splicing of the poly-A tail which prevents further splicing. 
C)  Methylation of the single intron prevents further splicing. 
D)  Removal of a single intron only leads to one possible outcome for spliced mRNA. 
E)  None of the above.

Question 11

The exons of a gene are always expressed in a functional protein.

Accepted Answer

A) True 
 B)False

Question 12

What basal transcription factor is most often affected by regulatory transcription factors?

Accepted Answer

A)  TFIIB 
B)  TFIID 
C)  TFIIE 
D)  TFIIF 
A)  TFIIB 
B)  TFIID 
C)  TFIIE 
D)  TFIIF

Question 13

Receptors for steroid hormones are usually found in the nucleus of the cell.

Accepted Answer

A) True 
 B)False

Question 14

Steroid hormomes are an example of an effector which regulates regulatory transcription factor
activity.

Accepted Answer

A) True 
 B)False

Question 15

A repressor protein would enhance the ability of TFIID to bind to the TATA box of the promoter.

Accepted Answer

A) True 
 B)False

Question 16

Which of the following is a steroid receptor?

Accepted Answer

A)  GRE 
B)  IRE 
C)  CRE 
D)  None of the answers are correct 
A)  GRE 
B)  IRE 
C)  CRE 
D)  None of the answers are correct

Question 17

A mutation in which of the following would result in little or no expression of a gene regulated by a CRE?

Accepted Answer

A)  G protein 
B)  Adenylyl cyclase 
C)  Protein kinase A 
D)  All of the answers are correct 
A)  G protein 
B)  Adenylyl cyclase 
C)  Protein kinase A 
D)  All of the answers are correct

Question 18

DNA that contains actively transcribed genes would most likely contain chromatin in the closed
configuration.

Accepted Answer

A) True 
 B)False

Question 19

cAMP is known as a second messenger system since the pathway is first activated by a extracellular
signaling molecule

Accepted Answer

A) True 
 B)False

Question 20

Histone acetyltransferases would be directly involved in which of the following?

Accepted Answer

A)  Formation of open chromatin 
B)  Movement of the nucleosome 
C)  Acetylation of lysines 
D)  Termination of gene expression 
A)  Formation of open chromatin 
B)  Movement of the nucleosome 
C)  Acetylation of lysines 
D)  Termination of gene expression

Exam 15: Gene Regulation in Eukaryotes

Activator proteins bind to silencer sequences and repressor proteins bind to enhancer sequences.

A heterodimer occurs when two identical transcription factors interact on a sequence of DNA. FALSE

Which of the following is incorrect regarding the glucocorticoid hormomes?

In which of the following scenarios would gene expression be the lowest?

eIF2a is phosphorylated in order to inhibit transcription. What is occurring at the molecular level?

What structural motifs promote dimerization?

Which mechanisms are used by miRNAs to regulate gene expression?

Genomic imprinting is a result of .

Based on the following mature mRNAs, what exons are constitutive? I. 1-2-3-4-7-8-10 II. 2-4-5-6-7-9 III. 1-4-6-7-8 IV. 1-2-4-7-10

Explain why yeast genes with a single intron have essentially no alternative splicing.

The exons of a gene are always expressed in a functional protein.

What basal transcription factor is most often affected by regulatory transcription factors?

Receptors for steroid hormones are usually found in the nucleus of the cell.

Steroid hormomes are an example of an effector which regulates regulatory transcription factor activity.

A repressor protein would enhance the ability of TFIID to bind to the TATA box of the promoter.

Which of the following is a steroid receptor?

A mutation in which of the following would result in little or no expression of a gene regulated by a CRE?

DNA that contains actively transcribed genes would most likely contain chromatin in the closed configuration.

cAMP is known as a second messenger system since the pathway is first activated by a extracellular signaling molecule

Histone acetyltransferases would be directly involved in which of the following?

Overview of Genetics

Mendelian Inheritance

Reproduction and Chromosome Transmission

Extensions of Mendelian Inheritance

Non-Mendelian Inheritance

Genetic Linkage and Mapping in Eukaryotes

Genetic Transfer and Mapping in Bacteria and

Variation in Chromosome Structure and Number

Molecular Structure of Dna and Rna

Chromosome Organization and Molecular Structure

Dna Replication

Gene Transcription and Rna Modification

Translation of Mrna

Gene Regulation in Bacteria and Bacteriophages

Gene Mutation and Dna Repair

Recombination and Transposition at the Molecular

Recombinant Dna Technology

Biotechnology

Genomics I: Analysis of Dna

Genomics II: Functional Genomics, Proteomics, and

Medical Genetics and Cancer

Developmental Genetics

Population Genetics

Quantitative Genetics

Evolutionary Genetics

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