Question 1

Which of the following is an example of a processive enzyme?

Accepted Answer

A)  DNA polymerase I 
B)  DNA polymerase III 
C)  DNA ligase 
D)  Okazaki fragments 
A)  DNA polymerase I 
B)  DNA polymerase III 
C)  DNA ligase 
D)  Okazaki fragments B

Question 2

You have discovered a strain of

Accepted Answer

A)  a 
B)  b 
C)  g 
D)  y 
E)  coli that grows very slowly-the generation time is nearly 12 hours compared to the normal 20-30 minutes. Upon further investigation, you find a mutation in the DNA
Polymerase III gene. What subunit of the holoenzyme does this mutation affect the most? 
A)  a 
B)  b 
C)  g 
D)  y 
E)  coli that grows very slowly-the generation time is nearly 12 hours compared to the normal 20-30 minutes. Upon further investigation, you find a mutation in the DNA
Polymerase III gene. What subunit of the holoenzyme does this mutation affect the most? B

Question 3

You extract DNA from an E.coli cell and observe it is hemi-methylated. Which strand of DNA is older?

Accepted Answer

A)  The methylated strand 
B)  The strand that is not methylated 
C)  Neither-they are the same "age." 
A)  The methylated strand 
B)  The strand that is not methylated 
C)  Neither-they are the same "age." A

Question 4

DNA polymerase III can synthesize a new strand of DNA at the rate of 300 nucleotides per minute.

Accepted Answer

A) True 
 B)False

Question 5

DNA polymerases add new nucleotides in what direction?

Accepted Answer

A)  5' to 3' 
B)  3 ' to 5' 
C)  Both directions 
A)  5' to 3' 
B)  3 ' to 5' 
C)  Both directions

Question 6

The proofreading of the DNA occurs in the .

Accepted Answer

A)  5' to 3' direction 
B)  3' to 5' direction 
C)  Both directions 
A)  5' to 3' direction 
B)  3' to 5' direction 
C)  Both directions

Question 7

Which of the following best describes the mechanism of DNA replication in which both parental strands remain together following replication?

Accepted Answer

A)  Dispersive 
B)  Semi-conservative 
C)  Conservative 
D)  All of the answers are correct 
A)  Dispersive 
B)  Semi-conservative 
C)  Conservative 
D)  All of the answers are correct

Question 8

In eukaryotes, which of the following is similar to the oriC region of prokaryotes?

Accepted Answer

A)  Dam 
B)  ARS elements 
C)  Promoters 
D)  Telomeres 
A)  Dam 
B)  ARS elements 
C)  Promoters 
D)  Telomeres

Question 9

Which of the following best describes the mechanism of DNA replication in which both a parental strand and daughter strand are combined following replication?

Accepted Answer

A)  Dispersive 
B)  Semi-conservative 
C)  Conservative 
D)  All of the answers are correct 
A)  Dispersive 
B)  Semi-conservative 
C)  Conservative 
D)  All of the answers are correct

Question 10

Bacterial DNA has how many origins of replication?

Accepted Answer

A)  0 
B)  1 
C)  10 
D)  Depends on the size of the DNA 
A)  0 
B)  1 
C)  10 
D)  Depends on the size of the DNA

Question 11

You have isolated what appears to be alien DNA. While studying its replication, you performed the exact experiment Meselson and Stahl did. After three generations, the DNA is subjected to a CsCl gradient and only one band appears. What type of replication does this DNA undergo?

Accepted Answer

A)  Semiconservative 
B)  Conservative 
C)  Dispersive 
A)  Semiconservative 
B)  Conservative 
C)  Dispersive

Question 12

What types of mutants were essential to the discovery of new replication enzymes?

Accepted Answer

A)  Gain-of-function mutations 
B)  Lethal mutations 
C)  Temperature-sensitive mutations 
D)  None of the answers are correct 
A)  Gain-of-function mutations 
B)  Lethal mutations 
C)  Temperature-sensitive mutations 
D)  None of the answers are correct

Question 13

The purpose of DNA replication is to produce .

Accepted Answer

A)  Two daughter strands 
B)  Two parental strands 
C)  Two template strands 
D)  None of the answers are correct 
A)  Two daughter strands 
B)  Two parental strands 
C)  Two template strands 
D)  None of the answers are correct

Question 14

A primosome consists of a polymerase and a single-stranded binding protein.

Accepted Answer

A) True 
 B)False

Question 15

Which of the following is not correct concerning the initiation of bacterial replication?

Accepted Answer

A)  It involves a region of the DNA called oriC 
B)  DNA proteins bind to the DNA to begin separation of the strands 
C)  The strands are initially separated at GC-rich regions of DNA 
D)  Following initial separation, DNA helicase enzymes continue to unwrap the DNA 
A)  It involves a region of the DNA called oriC 
B)  DNA proteins bind to the DNA to begin separation of the strands 
C)  The strands are initially separated at GC-rich regions of DNA 
D)  Following initial separation, DNA helicase enzymes continue to unwrap the DNA

Question 16

GGGCCATTCGAACGTCCGAAAATGCCCCTGAATGAAAATTTTGGCCC. The primer used for replication in vitro is CCCGGTAAGCTT. Where is the 5' end for the template and primer, respectively?

Accepted Answer

A)  Left, left 
B)  Right, left 
C)  Left, right 
D)  Right, right 
A)  Left, left 
B)  Right, left 
C)  Left, right 
D)  Right, right

Question 17

Catenanes can only form in cells with circular chromosomes, such as bacteria.

Accepted Answer

A) True 
 B)False

Question 18

Attaches adjacent Okazaki fragments, forming a continuous DNA strand.

Accepted Answer

A)  DNA ligase 
B)  DNA primase 
C)  Topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III 
A)  DNA ligase 
B)  DNA primase 
C)  Topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III

Question 19

DNA polymerase III has the ability to begin synthesis of the new daughter strands immediately following the formation of the replication fork.

Accepted Answer

A) True 
 B)False

Question 20

Fills in small regions of DNA where the RNA primers were located.

Accepted Answer

A)  DNA ligase 
B)  DNA primase 
C)  Topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III 
A)  DNA ligase 
B)  DNA primase 
C)  Topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III

Exam 11: Dna Replication

Which of the following is an example of a processive enzyme?

You have discovered a strain of

You extract DNA from an E.coli cell and observe it is hemi-methylated. Which strand of DNA is older?

DNA polymerase III can synthesize a new strand of DNA at the rate of 300 nucleotides per minute.

DNA polymerases add new nucleotides in what direction?

The proofreading of the DNA occurs in the .

Which of the following best describes the mechanism of DNA replication in which both parental strands remain together following replication?

In eukaryotes, which of the following is similar to the oriC region of prokaryotes?

Which of the following best describes the mechanism of DNA replication in which both a parental strand and daughter strand are combined following replication?

Bacterial DNA has how many origins of replication?

You have isolated what appears to be alien DNA. While studying its replication, you performed the exact experiment Meselson and Stahl did. After three generations, the DNA is subjected to a CsCl gradient and only one band appears. What type of replication does this DNA undergo?

What types of mutants were essential to the discovery of new replication enzymes?

The purpose of DNA replication is to produce .

A primosome consists of a polymerase and a single-stranded binding protein.

Which of the following is not correct concerning the initiation of bacterial replication?

GGGCCATTCGAACGTCCGAAAATGCCCCTGAATGAAAATTTTGGCCC. The primer used for replication in vitro is CCCGGTAAGCTT. Where is the 5' end for the template and primer, respectively?

Catenanes can only form in cells with circular chromosomes, such as bacteria.

Attaches adjacent Okazaki fragments, forming a continuous DNA strand.

DNA polymerase III has the ability to begin synthesis of the new daughter strands immediately following the formation of the replication fork.

Fills in small regions of DNA where the RNA primers were located.

Overview of Genetics

Mendelian Inheritance

Reproduction and Chromosome Transmission

Extensions of Mendelian Inheritance

Non-Mendelian Inheritance

Genetic Linkage and Mapping in Eukaryotes

Genetic Transfer and Mapping in Bacteria and

Variation in Chromosome Structure and Number

Molecular Structure of Dna and Rna

Chromosome Organization and Molecular Structure

Gene Transcription and Rna Modification

Translation of Mrna

Gene Regulation in Bacteria and Bacteriophages

Gene Regulation in Eukaryotes

Gene Mutation and Dna Repair

Recombination and Transposition at the Molecular

Recombinant Dna Technology

Biotechnology

Genomics I: Analysis of Dna

Genomics II: Functional Genomics, Proteomics, and

Medical Genetics and Cancer

Developmental Genetics

Population Genetics

Quantitative Genetics

Evolutionary Genetics

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