Exam 13: Dna Replication and Repair

The DNA strand growing toward the replication fork grows ______ in a 5'->3' direction as the replication fork advances and is called the ________.

What discovery suggested the presence of other DNA polymerases in bacteria besides the Kornberg DNA polymerase enzyme?

Cultured mammalian cells grown in thymidine for many generations were allowed to undergo replication in the presence of bromodeoxyuridine (BrdU), which replaces thymidine in DNA. After two rounds of replication, what would the DNA look like if replication were conservative?

A double-stranded breakage repair pathway that requires the presence of a second chromosome carrying the same sequence of genes as the damaged chromosome is called _________.

As replication proceeds, the lagging strand template of the DNA is looped back on itself so that it has the same orientation as the leading strand template; the looping DNA repeatedly grows and shortens during lagging strand replication. This model is often referred to as the _________.

At what stage of the eukaryotic cell cycle do key protein kinases, like cyclin-dependent kinase, become activated to allow for DNA replication initiation?

What is the average rate of DNA replication in a bacterium like E. coli?

Replication moves outward from the origin of replication in ________ direction(s)and is said to be ________.

Which enzyme is responsible for joining the last deoxyribonucleotide added during RNA primer digestion to the 5' end of the previously synthesized and adjacent DNA fragment?

Using DNA to store data has the disadvantage of __________________. (Select all correct choices)

After cuts are made on the two sides of the lesion in nucleotide excision repair, what holds the damaged part of the strand in position for a while before its final removal?

The prokaryotic enzyme DNA polymerase I can incorporate the wrong nucleotide occasionally. What is the probability that the wrong nucleotide is incorporated into the growing polynucleotide chain during replication?

In replicating bacterial chromosomes, where does replication terminate?

In bacteria, a _______ is formed when a ________ associates transiently with a _______.

Which DNA polymerase in bacteria is mostly involved in DNA repair to correct damaged DNA sections and removes RNA primers at the 5' ends of Okazaki fragments, replacing them with DNA?

Which eukaryotic DNA polymerase replicates mitochondrial DNA and does not appear to be involved in nuclear DNA replication?

What happens once damage caused by lesions in the genome, like a thymidine dimer, is bypassed during replication and replication continues?

In mammalian cells, a complex of proteins binds to the broken ends of the DNA duplex and catalyzes a series of reactions that rejoin the broken strands. This is an example of what kind of repair?

Which eukaryotic DNA polymerase is tightly associated with primase and responsible for Okazaki fragment synthesis initiation?

Cultured mammalian cells grown in thymidine for many generations were allowed to undergo replication in the presence of bromodeoxyuridine (BrdU), which replaces thymidine in DNA. After one round of replication, what would the DNA look like if replication were dispersive?