Bacteria are grown in a medium containing [15N]H4Cl for a number of generations so that all of the DNA is made of fully 'heavy' DNA. The bacteria are moved to a new medium and grown in [14N]H4Cl so that all subsequent new DNA will be 'light.' If replication were dispersive, what would the DNA look like after two generation times?

A) All of the DNA is made of two 'light' strands. B) Half of the DNA is made of two 'light' strands and half of the DNA is made of one 'light' strand and one 'heavy' strand. C) All of the DNA is made of one 'heavy' strand and one 'light' strand. D) Each strand is made of a mixture of 'heavy' and 'light' DNA, with each strand being between 75% and 100% 'light.' E) Three quarters of the DNA is made of two 'light' strands and one quarter is made of two 'heavy' strands. A) All of the DNA is made of two 'light' strands. B) Half of the DNA is made of two 'light' strands and half of the DNA is made of one 'light' strand and one 'heavy' strand. C) All of the DNA is made of one 'heavy' strand and one 'light' strand. D) Each strand is made of a mixture of 'heavy' and 'light' DNA, with each strand being between 75% and 100% 'light.' E) Three quarters of the DNA is made of two 'light' strands and one quarter is made of two 'heavy' strands.

Exam 13: Dna Replication and Repair

The DNA polymerases is a relatively large family of polymerases in which each member is specialized for incorporating nucleotides opposite particular types of DNA lesions in the template strand engage in a type of DNA synthesis called . They have an unusually spacious active site that __.

(Multiple Choice)

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Question 21

What supplies the energy that drives the relief of mechanical strain by DNA gyrase?

(Multiple Choice)

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Question 22

Cultured mammalian cells grown in thymidine for many generations were allowed to undergo replication in the presence of bromodeoxyuridine (BrdU), which replaces thymidine in DNA. After two rounds of replication, what does the DNA look like?

(Multiple Choice)

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Question 23

What is the probability that, in E. coli, an incorrect nucleotide will be incorporated into DNA during replication and remain there?

(Multiple Choice)

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Question 24

What is responsible for joining eukaryotic Okazaki fragments together?

(Multiple Choice)

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Question 25

How can the replication of both DNA strands by two tethered polymerases be accomplished when the template strands run in opposite directions?

(Multiple Choice)

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Question 26

What is the advantage of transcription-coupled repair?

(Multiple Choice)

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Question 27

Patients with the classical form of xeroderma pigmentosum have a defect in one of the genes involved in ______________.

(Multiple Choice)

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Question 28

___ are DNA unwinding enzymes that unwind the DNA in a reaction using the energy from to move along one of the DNA strands, breaking the _ holding the two strands together.

(Multiple Choice)

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Question 29

Which eukaryotic DNA polymerase is thought to be the primary DNA-synthesizing enzyme during replication of the lagging strand?

(Multiple Choice)

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Question 30

What is responsible for filling the gap left by the nucleases that digest the primer and the short DNA segment added to it by polymerase δ?

(Multiple Choice)

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Question 31

What is the name of the noncatalytic component of the DNA polymerase III holoenzyme that keeps the polymerase associated with the DNA template?

(Multiple Choice)

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Question 32

Why did Kornberg initially think that the exonuclease activity he found in his DNA polymerase preparations was a contaminating enzyme?

(Multiple Choice)

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Question 33

Which type of DNA repair removes, via a cut-and-patch mechanism, a variety of bulky lesions, like pyrimidine dimers and nucleotides to which various chemical groups have been attached?

(Multiple Choice)

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Question 34

What binds to the ORC to assemble the prereplication complex (pre-RC)that is competent to initiate replication?

(Multiple Choice)

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Question 35

Why is an intact, linear double helix an ineffective template for DNA polymerase?

(Multiple Choice)

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Question 36

What is the mechanism of action of the anti-HIV drug AZT?

(Multiple Choice)

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Question 37

Bacteria are grown in a medium containing [¹⁵N]H₄Cl for a number of generations so that all of the DNA is made of fully 'heavy' DNA. The bacteria are moved to a new medium and grown in [¹⁴N]H₄Cl so that all subsequent new DNA will be 'light.' If replication were dispersive, what would the DNA look like after two generation times?

(Multiple Choice)

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Question 38

Which of the following is an advantage of using RNA primers during initiation of a strand in replication?

(Multiple Choice)

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Question 39

Who was the first to suggest semiconservative replication?

(Multiple Choice)

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Question 40

Showing 21 - 40 of 98

The DNA polymerases is a relatively large family of polymerases in which each member is specialized for incorporating nucleotides opposite particular types of DNA lesions in the template strand engage in a type of DNA synthesis called . They have an unusually spacious active site that __.

What supplies the energy that drives the relief of mechanical strain by DNA gyrase?

Cultured mammalian cells grown in thymidine for many generations were allowed to undergo replication in the presence of bromodeoxyuridine (BrdU), which replaces thymidine in DNA. After two rounds of replication, what does the DNA look like?

What is the probability that, in E. coli, an incorrect nucleotide will be incorporated into DNA during replication and remain there?

What is responsible for joining eukaryotic Okazaki fragments together?

How can the replication of both DNA strands by two tethered polymerases be accomplished when the template strands run in opposite directions?

What is the advantage of transcription-coupled repair?

Patients with the classical form of xeroderma pigmentosum have a defect in one of the genes involved in ______________.

___ are DNA unwinding enzymes that unwind the DNA in a reaction using the energy from to move along one of the DNA strands, breaking the _ holding the two strands together.

Which eukaryotic DNA polymerase is thought to be the primary DNA-synthesizing enzyme during replication of the lagging strand?

What is responsible for filling the gap left by the nucleases that digest the primer and the short DNA segment added to it by polymerase δ?

What is the name of the noncatalytic component of the DNA polymerase III holoenzyme that keeps the polymerase associated with the DNA template?

Why did Kornberg initially think that the exonuclease activity he found in his DNA polymerase preparations was a contaminating enzyme?

Which type of DNA repair removes, via a cut-and-patch mechanism, a variety of bulky lesions, like pyrimidine dimers and nucleotides to which various chemical groups have been attached?

What binds to the ORC to assemble the prereplication complex (pre-RC)that is competent to initiate replication?

Why is an intact, linear double helix an ineffective template for DNA polymerase?

What is the mechanism of action of the anti-HIV drug AZT?

Which of the following is an advantage of using RNA primers during initiation of a strand in replication?

Who was the first to suggest semiconservative replication?

Introduction to the Study of Cell and Molecular Biology

The Chemical Basis of Life

Bioenergetics, Enzymes and Metabolism

The Structure and Function of the Plasma Membrane

Aerobic Respiration and the Mitochondrion

Photosynthesis and the Chloroplast

Interactions Between Cells and Their Environment

Cytoplasmic Membrane Systems: Structure, Function, and Membrane Trafficking

The Cytoskeleton and Cell Motility

The Nature of the Gene and the Genome

Gene Expression: From Transcription to Translation

Control of Gene Expression

Cellular Reproduction

Cell Signaling and Signal Transduction: Communication Between Cells

Cancer

The Immune Response

Techniques in Cell and Molecular Biology

Filters

Exam 13: Dna Replication and Repair

What supplies the energy that drives the relief of mechanical strain by DNA gyrase?

Cultured mammalian cells grown in thymidine for many generations were allowed to undergo replication in the presence of bromodeoxyuridine (BrdU), which replaces thymidine in DNA. After two rounds of replication, what does the DNA look like?

What is the probability that, in E. coli, an incorrect nucleotide will be incorporated into DNA during replication and remain there?

What is responsible for joining eukaryotic Okazaki fragments together?

How can the replication of both DNA strands by two tethered polymerases be accomplished when the template strands run in opposite directions?

What is the advantage of transcription-coupled repair?

Patients with the classical form of xeroderma pigmentosum have a defect in one of the genes involved in ______________.

_________ are DNA unwinding enzymes that unwind the DNA in a reaction using the energy from ________ to move along one of the DNA strands, breaking the _______ holding the two strands together.

Which eukaryotic DNA polymerase is thought to be the primary DNA-synthesizing enzyme during replication of the lagging strand?

What is responsible for filling the gap left by the nucleases that digest the primer and the short DNA segment added to it by polymerase δ?

What is the name of the noncatalytic component of the DNA polymerase III holoenzyme that keeps the polymerase associated with the DNA template?

Why did Kornberg initially think that the exonuclease activity he found in his DNA polymerase preparations was a contaminating enzyme?

Which type of DNA repair removes, via a cut-and-patch mechanism, a variety of bulky lesions, like pyrimidine dimers and nucleotides to which various chemical groups have been attached?

What binds to the ORC to assemble the prereplication complex (pre-RC)that is competent to initiate replication?

Why is an intact, linear double helix an ineffective template for DNA polymerase?

What is the mechanism of action of the anti-HIV drug AZT?

Which of the following is an advantage of using RNA primers during initiation of a strand in replication?

Who was the first to suggest semiconservative replication?

Introduction to the Study of Cell and Molecular Biology

The Chemical Basis of Life

Bioenergetics, Enzymes and Metabolism

The Structure and Function of the Plasma Membrane

Aerobic Respiration and the Mitochondrion

Photosynthesis and the Chloroplast

Interactions Between Cells and Their Environment

Cytoplasmic Membrane Systems: Structure, Function, and Membrane Trafficking

The Cytoskeleton and Cell Motility

The Nature of the Gene and the Genome

Gene Expression: From Transcription to Translation

Control of Gene Expression

Cellular Reproduction

Cell Signaling and Signal Transduction: Communication Between Cells

Cancer

The Immune Response

Techniques in Cell and Molecular Biology

Filters

___ are DNA unwinding enzymes that unwind the DNA in a reaction using the energy from to move along one of the DNA strands, breaking the _ holding the two strands together.