Exam 13: Dna Replication and Repair

Strand initiation during replication is carried out by an enzyme that makes a short RNA molecule that is used as a primer; the enzyme is a distinct type of RNA polymerase, called _______.

Which piece of evidence provided the best evidence that the sequence of DNA itself was unimportant in the timing of replication?

Which DNA repair mechanism recognizes a distortion in double helix geometry caused by DNA polymerase's insertion of an incorrect nucleotide during replication, one that escaped the enzyme's proofreading exonuclease?

In early experiments with DNA polymerase I, what evidence convinced investigators that the original unlabeled DNA in the reaction mixture served as the template for the newly made DNA?

The specific site on the bacterial chromosome at which replication begins is called the ________ of replication.

What technique was used to show DNA molecules are replicated simultaneously at several sites along their length?

How does DNA polymerase III move from one site on the lagging strand template to a site closer to replication fork?

The assembly of the β clamp around the DNA requires a multisubunit _______ that is also part of the ________; part of this structure opens the ______ so that it can fit around the DNA.

The DNA polymerase on the leading strand template _____________.

Autonomous replicating sequences that have been isolated and analyzed share several distinct elements. Which distinct elements below are shared by autonomous replicating sequences? (Select all correct choices)

Which of these crops is NOT a product of the gamma garden experiments?

What happens simultaneously with the removal of RNA primer by the 5'->3' exonuclease activity of DNA polymerase I?

Since one strand of DNA is synthesized continuously during replication and the other is made discontinuously, replication is said to be ___________.

Where is the DnaB helicase first loaded onto the DNA?

Which side chain critical to DNA strand elongation is lacking in the nucleotide analog azidothymidine (AZT)?

Cultured mammalian cells grown in thymidine for many generations were allowed to undergo replication in the presence of bromodeoxyuridine (BrdU), which replaces thymidine in DNA. After one round of replication, what does the DNA look like?

The small portions in which eukaryotic cells replicate their genomes are called _______.

Which type of replication results in 2 duplexes made of one parental strand and one newly synthesized strand?

Using DNA to store data has the advantage of __________________. (Select all correct choices)

The enzyme that joins the small fragments of the lagging strand together into a continuous strand is called _______.