Exam 18: Non-Coding Rnas

Into which gene of the CRISPR-Cas system are fragments of bacteriophage DNA inserted?

SmY RNAs are a family of ncRNAs in nematodes that are thought to be involved in a special type of RNA splicing.Based on this function,SmY RNAs are most likely members of which category of ncRNAs?

Select functions that ncRNAs can perform.Choose all that apply.

Select the ncRNA that facilitates the binding of telomerase to the telomere and acts as a template for DNA replication.

Select the ways in which modifications to the chromatin brought about by HOTAIR and associated proteins are proposed to inhibit transcription.

With which injection did Mello and Fire observe the lowest amount of mex-3 probe staining?

In order of their occurrence what are the steps in RNA interference?

Consider the two key roles that have been identified for SRP RNA.You have isolated a mutant SRP RNA.This RNA is able to perform its role as a scaffold for SRP proteins,but has lost its other function.How will this affect translation of SRP-containing proteins?

Abnormal levels of miRNAs are associated with nearly all forms of what type of human disease?

What is the name of the type of chemical modification on anti-miRNA oligonucleotides used to inhibit miRNA function that involves ribose sugars with an extra bridge connecting the 2′ oxygen and the 4′ carbon?

Select possible outcomes after RISC binds to an mRNA during RNA interference.Choose all that apply.

In an in vitro translation assay radioactive amino acids can be incorporated into proteins and the proteins separated from the unincorporated amino acids (higher counts per minute indicates more protein synthesis).Given the following data what are the conclusions that you would come to?  

You work in a lab that is studying piRISCs and PIWI proteins.You have isolated piRISCs from a cell. What would be one way to identify which piRISCs are involved in cleaving TE RNA vs.those that halt transcription?

What human disease is associated with mutations in Drosha?

The ncRNA HOTAIR recruits what type of proteins to target genes?

Based on what you know about cartilage-hair hypoplasia,which strategy involving ncRNAs do you anticipate would be useful in treating this disease?

You are working in a lab that studies ncRNAs.You develop an assay to isolate ncRNAs and any associated molecules.You must then identify the molecules you have isolated.You find a molecule that is associated with an ncRNA that is not changed by protease,RNase,or DNase.The associated molecule is most likely

Ribozymes are RNA molecules with what type of activity?

piRNA sequences are complimentary to

A bacterial ncRNA called OxyS regulates translation by binding to the Shine-Dalgarno sequence,which prevents ribosome binding.What is the function of this ncRNA?