Question 1

cAMP is a small effector molecule.

Accepted Answer

A) True 
 B)False  True

Question 2

In the Jacob Monod merozygote experiment,what was indicated by the presence of a yellow color when b-ONPG was added?

Accepted Answer

A)  Expression of the lac operon is constitutive whether lacI is functional or not. 
B)  Beta-galactosidase was present. 
C)  The lac operon was completely turned off. 
D)  The researcher added too much b-ONPG. 
A)  Expression of the lac operon is constitutive whether lacI is functional or not. 
B)  Beta-galactosidase was present. 
C)  The lac operon was completely turned off. 
D)  The researcher added too much b-ONPG. B

Question 3

If CAP could not bind to its CAP site,then what would be the result? Assume lactose is present in each scenario.

Accepted Answer

A)  Transcription would be difficult to repress in the presence of glucose. 
B)  Transcription would be difficult to activate in the presence of glucose. 
C)  Transcription would be difficult to activate in the absence of glucose. 
D)  Transcription would be difficult to repress in the absence of glucose. 
A)  Transcription would be difficult to repress in the presence of glucose. 
B)  Transcription would be difficult to activate in the presence of glucose. 
C)  Transcription would be difficult to activate in the absence of glucose. 
D)  Transcription would be difficult to repress in the absence of glucose. D

Question 4

Translational regulatory proteins recognize specific areas of what molecule?

Accepted Answer

A)  tRNA 
B)  Ribosome 
C)  rRNA 
D)  mRNA 
E)  None of the answers are correct. 
A)  tRNA 
B)  Ribosome 
C)  rRNA 
D)  mRNA 
E)  None of the answers are correct.

Question 5

Coupled transcription and translation occur under conditions in which the tryptophan concentration is very low.

Accepted Answer

A) True 
 B)False

Question 6

Which of the following is not an example of translational regulation in prokaryotes?

Accepted Answer

A)  sterically blocking the ribosome 
B)  phosphorylation of an enzyme 
C)  incorporation of antisense RNA 
D)  altering the structure of the mRNA 
A)  sterically blocking the ribosome 
B)  phosphorylation of an enzyme 
C)  incorporation of antisense RNA 
D)  altering the structure of the mRNA

Question 7

Riboswitches have been shown to have regulation of ________.

Accepted Answer

A)  transcription and translation 
B)  only transcription 
C)  only translation 
D)  only RNA splicing 
A)  transcription and translation 
B)  only transcription 
C)  only translation 
D)  only RNA splicing

Question 8

If a bacterium is placed in an environment that contains both glucose and lactose,the regulation of the lac operon will allow which nutrient to be processed first?

Accepted Answer

A)  glucose 
B)  lactose 
C)  Both will be processed equally. 
D)  Neither will be processed in this environment. 
A)  glucose 
B)  lactose 
C)  Both will be processed equally. 
D)  Neither will be processed in this environment.

Question 9

Operons that code for anabolic enzyme systems are typically regulated by inducers.

Accepted Answer

A) True 
 B)False

Question 10

A gene is inducible and under negative control.Which of the following pairs will allow expression of this gene?

Accepted Answer

A)  activator + repressor 
B)  activator + inhibitor 
C)  repressor + inducer 
D)  repressor + corepressor 
A)  activator + repressor 
B)  activator + inhibitor 
C)  repressor + inducer 
D)  repressor + corepressor

Question 11

Operons that code for catabolic enzyme systems are typically regulated by repressors.

Accepted Answer

A) True 
 B)False

Question 12

In the trp operon,tryptophan is a corepressor.

Accepted Answer

A) True 
 B)False

Question 13

How many promoters are in an operon?

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  It depends on how many genes there are in the operon. 
A)  1 
B)  2 
C)  3 
D)  It depends on how many genes there are in the operon.

Question 14

Which of the following is not associated with positive transcriptional regulation?

Accepted Answer

A)  activators 
B)  inducers 
C)  corepressors 
D)  All of the answers are involved in positive regulation. 
A)  activators 
B)  inducers 
C)  corepressors 
D)  All of the answers are involved in positive regulation.

Question 15

Enzymes involved in metabolism are most likely regulated via ________.

Accepted Answer

A)  feedback inhibition 
B)  acetylation 
C)  methylation 
D)  None of the answers are correct. 
A)  feedback inhibition 
B)  acetylation 
C)  methylation 
D)  None of the answers are correct.

Question 16

In the lac operon,the operator site is recognized by an activator protein.

Accepted Answer

A) True 
 B)False

Question 17

The regulation of protein function,not gene expression is called ________ regulation.

Accepted Answer

A)  posttranslational 
B)  transcriptional 
C)  translational 
D)  posttranscriptional 
A)  posttranslational 
B)  transcriptional 
C)  translational 
D)  posttranscriptional

Question 18

Antisense RNA does which of the following?

Accepted Answer

A)  inhibits the formation of the open complex in transcription 
B)  occupies the A and P sites of the ribosome 
C)  binds to the mRNA and prevents translation 
D)  prevents the correct folding of a newly formed peptide 
A)  inhibits the formation of the open complex in transcription 
B)  occupies the A and P sites of the ribosome 
C)  binds to the mRNA and prevents translation 
D)  prevents the correct folding of a newly formed peptide

Question 19

The form of regulation that involves a physical change in the shape of an enzyme is called allosteric regulation.

Accepted Answer

A) True 
 B)False

Question 20

Which of the following encode polycistronic mRNA?

Accepted Answer

A)  lac operon 
B)  ara operon 
C)  trp operon 
D)  All of the answers are correct. 
A)  lac operon 
B)  ara operon 
C)  trp operon 
D)  All of the answers are correct.

Exam 16: Gene Regulation in Bacteria

cAMP is a small effector molecule.

In the Jacob Monod merozygote experiment,what was indicated by the presence of a yellow color when b-ONPG was added?

If CAP could not bind to its CAP site,then what would be the result? Assume lactose is present in each scenario.

Translational regulatory proteins recognize specific areas of what molecule?

Coupled transcription and translation occur under conditions in which the tryptophan concentration is very low.

Which of the following is not an example of translational regulation in prokaryotes?

Riboswitches have been shown to have regulation of ________.

If a bacterium is placed in an environment that contains both glucose and lactose,the regulation of the lac operon will allow which nutrient to be processed first?

Operons that code for anabolic enzyme systems are typically regulated by inducers.

A gene is inducible and under negative control.Which of the following pairs will allow expression of this gene?

Operons that code for catabolic enzyme systems are typically regulated by repressors.

In the trp operon,tryptophan is a corepressor.

How many promoters are in an operon?

Which of the following is not associated with positive transcriptional regulation?

Enzymes involved in metabolism are most likely regulated via ________.

In the lac operon,the operator site is recognized by an activator protein.

The regulation of protein function,not gene expression is called ________ regulation.

Antisense RNA does which of the following?

The form of regulation that involves a physical change in the shape of an enzyme is called allosteric regulation.

Which of the following encode polycistronic mRNA?

Overview of Genetics

Reproduction and Chromosome Transmission

Mendelian Inheritance

Sex Determination and Sex Chromosomes

Extensions of Mendelian Inheritance

Extranuclear Inheritance, Imprinting, and Maternal Effect

Genetic Linkage and Mapping in Eukaryotes

Variation in Chromosome Structure and Number

Genetics in Bacteria

Genetics of Viruses

Molecular Structure of DNA and RNA

Molecular Structure of Chromosomes and Transposition

Dna Replication and Recombination

Gene Transcription and RNA Modification

Translation of MRNA

Gene Regulation in Eukaryotes

Non-Coding Rnas

Gene Mutation and DNA Repair

Molecular Technologies

Genomics

Medical Genetics and Cancer

Population Genetics

Quantitative Genetics

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