Question 1

What does physical mapping provide that the other types of genetic mapping do not?

Accepted Answer

A)  recombination distances between genes 
B)  number of nucleotide base pairs between genes 
C)  relationship of the gene to specific chromosomal banding patterns 
D)  frequencies of alleles in a population 
A)  recombination distances between genes 
B)  number of nucleotide base pairs between genes 
C)  relationship of the gene to specific chromosomal banding patterns 
D)  frequencies of alleles in a population B

Question 2

After a metagenomics researcher collects a mixed sample of DNA,what next step allows this DNA to be studied?

Accepted Answer

A)  The DNA fragments are each ligated into a cloning vector to create a DNA library. 
B)  The pieces of DNA are ligated together into one complete "genome" so they can be sequenced. 
C)  The species responsible for each DNA are identified and individually cultured. 
D)  DNA is reconstructed into chromosomes by using the human genome as a scaffold. 
A)  The DNA fragments are each ligated into a cloning vector to create a DNA library. 
B)  The pieces of DNA are ligated together into one complete "genome" so they can be sequenced. 
C)  The species responsible for each DNA are identified and individually cultured. 
D)  DNA is reconstructed into chromosomes by using the human genome as a scaffold. A

Question 3

________ uses the unique banding patterns of a chromosome to determine the general location of a gene.

Accepted Answer

A)  Physical mapping 
B)  Cytogenetic mapping 
C)  Linkage mapping 
D)  Shotgun sequencing 
A)  Physical mapping 
B)  Cytogenetic mapping 
C)  Linkage mapping 
D)  Shotgun sequencing B

Question 4

An application of FISH technique,where multiple DNA probes are labeled with different fluorescent molecules that bind to a chromosome,is called ________.Using this technique,researchers can determine the sites of the DNA segments along the chromosome.

Accepted Answer

A)  chromosome painting 
B)  Giemsa staining 
C)  pyrosequencing 
D)  physical mapping 
A)  chromosome painting 
B)  Giemsa staining 
C)  pyrosequencing 
D)  physical mapping

Question 5

The most common microsatellite in humans is ________.

Accepted Answer

A)  (AT)₁₀₀ 
B)  (C
A) n 
C)  (TG)n 
D)  (CAT)n 
E)  AUG 
A)  (AT)₁₀₀ 
B)  (C
A) n 
C)  (TG)n 
D)  (CAT)n 
E)  AUG

Question 6

Pyrosequencing is an example of a high-throughput,next-generation sequencing technology.What is a crucial detail of the procedure?

Accepted Answer

A)  including the enzyme luciferase 
B)  mixing the solution of four nucleotides together in even amounts 
C)  removing nucleotides from the target DNA one at a time 
D)  using large wells to accommodate multiple beads 
A)  including the enzyme luciferase 
B)  mixing the solution of four nucleotides together in even amounts 
C)  removing nucleotides from the target DNA one at a time 
D)  using large wells to accommodate multiple beads

Question 7

In sequencing by synthesis (SBS)methods,the sequence of a sample is directly determined as nucleotides are incorporated into a DNA strand by DNA polymerase.

Accepted Answer

A) True 
 B)False

Question 8

In a FISH procedure,the probe being used is labeled with a fluorescent tag.

Accepted Answer

A) True 
 B)False

Question 9

cDNA uses chromosomal DNA as its template.

Accepted Answer

A) True 
 B)False

Question 10

Gene chips are being used for which of the following purposes?

Accepted Answer

A)  to examine patterns of gene expression under various environmental conditions 
B)  to establish levels of genetic variation 
C)  to determine gene expression specific to certain cell types 
D)  to identify species of bacteria 
E)  All of these choices are possible. 
A)  to examine patterns of gene expression under various environmental conditions 
B)  to establish levels of genetic variation 
C)  to determine gene expression specific to certain cell types 
D)  to identify species of bacteria 
E)  All of these choices are possible.

Question 11

To identify novel DNA binding sites for a protein,it would be best to use ________.

Accepted Answer

A)  pyrosequencing 
B)  microsatellites 
C)  chromatin immunoprecipitation 
D)  ChIP-chip 
A)  pyrosequencing 
B)  microsatellites 
C)  chromatin immunoprecipitation 
D)  ChIP-chip

Question 12

The human genome consists of approximately ________ base pairs of DNA.

Accepted Answer

A)  100,000 
B)  1 million 
C)  1 billion 
D)  3 billion 
E)  2 trillion 
A)  100,000 
B)  1 million 
C)  1 billion 
D)  3 billion 
E)  2 trillion

Question 13

A researcher isolates mRNA from healthy and diseased liver biopsies.  Each mRNA sample is converted into cDNA and subjected to next-generation sequencing.Select all statements that describe this experiment and the possible results.

Accepted Answer

A)  The researcher can identify transcripts that are more abundant in healthy liver than in diseased liver, which are candidate genes for further study. 
B)  The researcher can identify transcripts that are less abundant in healthy liver than in diseased liver, which are candidate genes for further study. 
C)  This is an RNA-Seq experiment. 
D)  This is a DNA microarray analysis. 
E)  Some genes' transcripts may be present in both samples but have different splice forms in healthy and diseased liver. 
A)  The researcher can identify transcripts that are more abundant in healthy liver than in diseased liver, which are candidate genes for further study. 
B)  The researcher can identify transcripts that are less abundant in healthy liver than in diseased liver, which are candidate genes for further study. 
C)  This is an RNA-Seq experiment. 
D)  This is a DNA microarray analysis. 
E)  Some genes' transcripts may be present in both samples but have different splice forms in healthy and diseased liver.

Question 14

One map unit of distance always corresponds to 10⁶ base pairs.

Accepted Answer

A) True 
 B)False

Question 15

A microsatellite marker may be used to predict the likelihood that an individual carries a trait for a specific disease.

Accepted Answer

A) True 
 B)False

Question 16

RFLP stands for restriction fragment linked position.

Accepted Answer

A) True 
 B)False

Question 17

Match between columns
                        Premises: A site where a single nucleotide is variable between individuals
Individual variation in the size of PCR-amplified fragments
A site containing short repeated sequences
Individual variation in the distance between restriction sites
A marker that is found at only one site within the genome

Accepted Answer

The Answer of Microsatellite and STS and RFLP and SNP is...

Question 18

Part of the Human Genome Project involved sequencing the genomes of model organisms,including bacteria and a plant.

Accepted Answer

A) True 
 B)False

Question 19

________ make it possible for researchers to study how an entire genome responds to an environmental stimuli.

Accepted Answer

A)  Gene knockouts 
B)  PCR reactions 
C)  DNA microarrays 
D)  Shotgun sequences 
A)  Gene knockouts 
B)  PCR reactions 
C)  DNA microarrays 
D)  Shotgun sequences

Question 20

A site in the genome that varies between members of the population is said to be ________.

Accepted Answer

A)  monomorphic 
B)  polymorphic 
C)  trimorphic 
D)  neomorphic 
A)  monomorphic 
B)  polymorphic 
C)  trimorphic 
D)  neomorphic

Exam 21: Genomics

What does physical mapping provide that the other types of genetic mapping do not?

After a metagenomics researcher collects a mixed sample of DNA,what next step allows this DNA to be studied?

________ uses the unique banding patterns of a chromosome to determine the general location of a gene.

An application of FISH technique,where multiple DNA probes are labeled with different fluorescent molecules that bind to a chromosome,is called ________.Using this technique,researchers can determine the sites of the DNA segments along the chromosome.

The most common microsatellite in humans is ________.

Pyrosequencing is an example of a high-throughput,next-generation sequencing technology.What is a crucial detail of the procedure?

In sequencing by synthesis (SBS)methods,the sequence of a sample is directly determined as nucleotides are incorporated into a DNA strand by DNA polymerase.

In a FISH procedure,the probe being used is labeled with a fluorescent tag.

cDNA uses chromosomal DNA as its template.

Gene chips are being used for which of the following purposes?

To identify novel DNA binding sites for a protein,it would be best to use ________.

The human genome consists of approximately ________ base pairs of DNA.

A researcher isolates mRNA from healthy and diseased liver biopsies. Each mRNA sample is converted into cDNA and subjected to next-generation sequencing.Select all statements that describe this experiment and the possible results.

One map unit of distance always corresponds to 10⁶ base pairs.

A microsatellite marker may be used to predict the likelihood that an individual carries a trait for a specific disease.

RFLP stands for restriction fragment linked position.

Part of the Human Genome Project involved sequencing the genomes of model organisms,including bacteria and a plant.

________ make it possible for researchers to study how an entire genome responds to an environmental stimuli.

A site in the genome that varies between members of the population is said to be ________.

Overview of Genetics

Reproduction and Chromosome Transmission

Mendelian Inheritance

Sex Determination and Sex Chromosomes

Extensions of Mendelian Inheritance

Extranuclear Inheritance, Imprinting, and Maternal Effect

Genetic Linkage and Mapping in Eukaryotes

Variation in Chromosome Structure and Number

Genetics in Bacteria

Genetics of Viruses

Molecular Structure of DNA and RNA

Molecular Structure of Chromosomes and Transposition

Dna Replication and Recombination

Gene Transcription and RNA Modification

Translation of MRNA

Gene Regulation in Bacteria

Gene Regulation in Eukaryotes

Non-Coding Rnas

Gene Mutation and DNA Repair

Molecular Technologies

Medical Genetics and Cancer

Population Genetics

Quantitative Genetics

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