Exam 9: Genetics in Bacteria

P1 phage can carry a piece of the chromosome that is 2 minutes in length.In a P1 phage transduction experiment,two genes have a cotransduction frequency of 0.68.Determine the distance between these two genes,assuming that pieces of chromosome are packaged randomly.

This form of DNA transfer was first observed by Lederberg and Tatum (1946)in Escherichia coli.

Cotransduction establishes the general order of genes on a bacterial chromosome,while conjugation is more specific and thus may be used to establish precise distances.

An episome is a segment of bacterial DNA that can exist as a plasmid or be integrated into the bacterial chromosome.

A cell with an F factor integrated into the bacterial chromosome is called ________.

Who was the first to discover the process of natural transformation?

The process of conjugation may introduce new genes into the recipient strain,altering its genotype.

Which of the following matings would have the highest frequency of chromosomal gene transfer?

Small circular pieces of DNA,separate from the bacterial chromosome,are called ________.

Which does not require any recombination with the bacterial chromosome?

In experiments,interrupted mating may be used to 

DNA transfer via a physical connection between two bacteria is known as 

Cotransduction can only be used to map genes that are within 20 minutes of each other,because the phage can only package 50% of the genome at a time.

When a bacteria assimilates genetic material (DNA)directly from the environment,this is called

Loss of function mutations are easier to study in ________ organisms,such as bacteria.

Which process uses a bacteriophage as an intermediary for the genetic information?

After two cells make contact via the conjugation bridge,the protein complex that initiates DNA transfer is called the

The site on a plasmid that is cut,producing a single strand of DNA,to allow conjugation to occur is called the ________.

A minute is the basic unit of map distance in bacteria.

Based on the following data,what two genes are farthest apart?