Question 1

The image below represents a(n): ________.

Accepted Answer

A)  Dark field microscope 
B)  Bright field microscope 
C)  Fluorescence microscope 
D)  Phase contrast microscope 
A)  Dark field microscope 
B)  Bright field microscope 
C)  Fluorescence microscope 
D)  Phase contrast microscope B

Question 2

What type of microscope is shown below?

Accepted Answer

A)  Bright field microscope 
B)  Transmission electron microscope 
C)  Fluorescence microscope 
D)  Dark field microscope 
A)  Bright field microscope 
B)  Transmission electron microscope 
C)  Fluorescence microscope 
D)  Dark field microscope B

Question 3

What of the following is a correct statement about the major differences between electron and light microscopes? (Select all that apply)

Accepted Answer

A)  Electron microscopes use beams of electrons and electromagnetic lenses while light microscopes use visible light waves and glass lenses. 
B)  Electron microscopes can show detailed images at the level of the cellular organelles while light microscopes cannot do this. 
C)  Electron microscopes have a lower resolving power that light microscopes because an electron bean has a longer wavelength that visible light. 
D)  Electron microscopes produces images on a photographic plate while light microscopes produce images through the ocular lens. 
A)  Electron microscopes use beams of electrons and electromagnetic lenses while light microscopes use visible light waves and glass lenses. 
B)  Electron microscopes can show detailed images at the level of the cellular organelles while light microscopes cannot do this. 
C)  Electron microscopes have a lower resolving power that light microscopes because an electron bean has a longer wavelength that visible light. 
D)  Electron microscopes produces images on a photographic plate while light microscopes produce images through the ocular lens. A,B,D

Question 4

The bright field light micrograph below is an image of: ________.

Accepted Answer

A)  gram positive bacterial cells 
B)  gram negative bacterial cells 
C)  acid fast bacterial cells 
D)  bacterial cells with endospores 
A)  gram positive bacterial cells 
B)  gram negative bacterial cells 
C)  acid fast bacterial cells 
D)  bacterial cells with endospores

Question 5

The ________ holds the specimen in place on a light microscope.

Accepted Answer

A)  Iris diaphragm 
B)  Condenser lens 
C)  Objective and Ocular lenses 
D)  Stage 
A)  Iris diaphragm 
B)  Condenser lens 
C)  Objective and Ocular lenses 
D)  Stage

Question 6

Negative staining: ________. (Select all that apply)

Accepted Answer

A)  shows clear cells against darkly stained background 
B)  uses stains that are acidic and have a negative charge 
C)  works because the negative charge is repelled by negative charge of carbs and glycoproteins on bacterial surfaces 
D)  shows stained cells against a clear background 
A)  shows clear cells against darkly stained background 
B)  uses stains that are acidic and have a negative charge 
C)  works because the negative charge is repelled by negative charge of carbs and glycoproteins on bacterial surfaces 
D)  shows stained cells against a clear background

Question 7

Positive Staining technique: ________.

Accepted Answer

A)  shows clear cells against a darkly stained background 
B)  uses stains that are acidic such as nigrosine and Indian ink 
C)  uses stains that have a negative charge that is repelled by the negative charge of cell surface carbohydrates and glycoproteins 
D)  uses basic dyes such as Methylene Blue and Crystal Violet 
A)  shows clear cells against a darkly stained background 
B)  uses stains that are acidic such as nigrosine and Indian ink 
C)  uses stains that have a negative charge that is repelled by the negative charge of cell surface carbohydrates and glycoproteins 
D)  uses basic dyes such as Methylene Blue and Crystal Violet

Question 8

The very complex microscopes used in research are classified according to ________.

Accepted Answer

A)  how large and complex they are 
B)  how they generate a magnified image 
C)  type of stain required 
D)  whether they make use of probes 
A)  how large and complex they are 
B)  how they generate a magnified image 
C)  type of stain required 
D)  whether they make use of probes

Question 9

What are the advantages of Dark-field microscopy? (Select all that apply)

Accepted Answer

A)  It may be used for living microbes 
B)  It may be used for hard-to-stain microbes 
C)  It highlights only surfaces of microbes 
D)  It can be used to visualize very thin specimens 
A)  It may be used for living microbes 
B)  It may be used for hard-to-stain microbes 
C)  It highlights only surfaces of microbes 
D)  It can be used to visualize very thin specimens

Question 10

What is the effect of using immersion oil plus oil immersion objective? (Select all that apply)

Accepted Answer

A)  Resolving power is increased because immersion oil has same refractive index as glass. 
B)  As light passes through the glass of the cover slip, oil and the glass of the oil immersion lens, there is no light lost as a result of differences in refractive index. 
C)  Increases both magnification and resolving power of microscope. 
D)  Magnification increases from 1000X to 4500X. 
A)  Resolving power is increased because immersion oil has same refractive index as glass. 
B)  As light passes through the glass of the cover slip, oil and the glass of the oil immersion lens, there is no light lost as a result of differences in refractive index. 
C)  Increases both magnification and resolving power of microscope. 
D)  Magnification increases from 1000X to 4500X.

Question 11

Using the information from the image below, determine the magnification of the microbe.

Accepted Answer

A)  200X 
B)  2.2X 
C)  91X 
D)  110X 
A)  200X 
B)  2.2X 
C)  91X 
D)  110X

Question 12

Flagella staining requires ________ to make the flagella more visible.

Accepted Answer

A)  dye only in a single layer 
B)  dye plus mordent in a single layer of dye 
C)  mordent only in a single layer 
D)  dye plus mordant in multiple layers 
A)  dye only in a single layer 
B)  dye plus mordent in a single layer of dye 
C)  mordent only in a single layer 
D)  dye plus mordant in multiple layers

Question 13

Which of the following best defines the term "resolution"?

Accepted Answer

A)  The degree to which a microscope shows two images very far apart 
B)  The degree to which a microscope shows two or more cells as being distinct 
C)  The degree to which a microscope shows two or more cells as being close together 
D)  The degree to which a microscope shows two very close objects as being separate and apart 
A)  The degree to which a microscope shows two images very far apart 
B)  The degree to which a microscope shows two or more cells as being distinct 
C)  The degree to which a microscope shows two or more cells as being close together 
D)  The degree to which a microscope shows two very close objects as being separate and apart

Question 14

What is the correct order of microscope parts from the base to the ocular?

Accepted Answer

A)  Base, illuminator, condenser lens, iris diaphragm, stage, objective lens, ocular lens 
B)  Base, condenser lens, illuminator, iris diaphragm, objective lens, stage, ocular lens 
C)  Base, iris diaphragm, stage, illuminator, condenser lens, objective lens, ocular lens 
D)  Base, stage, condenser lens, illuminator, objective lens, stage, iris diaphragm, ocular lens 
A)  Base, illuminator, condenser lens, iris diaphragm, stage, objective lens, ocular lens 
B)  Base, condenser lens, illuminator, iris diaphragm, objective lens, stage, ocular lens 
C)  Base, iris diaphragm, stage, illuminator, condenser lens, objective lens, ocular lens 
D)  Base, stage, condenser lens, illuminator, objective lens, stage, iris diaphragm, ocular lens

Question 15

Which of the following statements is TRUE concerning Direct Fluorescent Antibody (DFA) Assay use in microscopy? (Select all that apply)

Accepted Answer

A)  Monoclonal antibodies bind to dyes and are then bound to pathogens 
B)  Antibody-dye conjugates glow under uv light and show presence of pathogen 
C)  Fluorescein and rhodamine are dyes most commonly used as they glow green and red 
D)  It is the simplest, most common type of microscope 
A)  Monoclonal antibodies bind to dyes and are then bound to pathogens 
B)  Antibody-dye conjugates glow under uv light and show presence of pathogen 
C)  Fluorescein and rhodamine are dyes most commonly used as they glow green and red 
D)  It is the simplest, most common type of microscope

Question 16

What is the correct order of steps in smear preparation?
1) Apply suspension of cells on slide
2) spread over surface of slide
3) heat fix or chemical fix specimen on slide
4) air dry

Accepted Answer

A)  1, 2, 4, 3 
B)  1, 2, 3, 4 
C)  1, 3, 2, 4 
D)  3, 2, 1, 4 
A)  1, 2, 4, 3 
B)  1, 2, 3, 4 
C)  1, 3, 2, 4 
D)  3, 2, 1, 4

Question 17

What dyes are used most often with fluorescence microscopy?

Accepted Answer

A)  Malachite green and carbol fuchsin 
B)  Fluorescein and rhodamine 
C)  Fluorescein and crystal violet 
D)  Crystal violet and safranin 
A)  Malachite green and carbol fuchsin 
B)  Fluorescein and rhodamine 
C)  Fluorescein and crystal violet 
D)  Crystal violet and safranin

Question 18

Differential Interference Contrast Microscopy (DIC) makes use of these features to enable microbiologists to view a living specimen: ________.

Accepted Answer

A)  changes in light waves as they pass through the living specimen are out of phase with each other 
B)  utilizes a bright-field microscope with filters 
C)  Three-dimensional color images generated 
D)  Magnification is higher than that of a bright-field microscope 
A)  changes in light waves as they pass through the living specimen are out of phase with each other 
B)  utilizes a bright-field microscope with filters 
C)  Three-dimensional color images generated 
D)  Magnification is higher than that of a bright-field microscope

Question 19

Which of the following statements about Atomic Force Microscopy is true? (Select all that apply)

Accepted Answer

A)  A fine-tipped probe is dragged across surface of molecules like proteins. 
B)  The movements of probe are amplified to produce an image. 
C)  The heights of a structure can be delineated relative to the background. 
D)  The voltage changes detected by the probe are converted to an image. 
A)  A fine-tipped probe is dragged across surface of molecules like proteins. 
B)  The movements of probe are amplified to produce an image. 
C)  The heights of a structure can be delineated relative to the background. 
D)  The voltage changes detected by the probe are converted to an image.

Question 20

What is the major difference between TEM and SEM?

Accepted Answer

A)  TEM uses light waves and electrons while SEM uses only electron beams. 
B)  TEM uses electromagnetic condenser lens as well as an electromagnetic objective lens while SEM uses only 2 electromagnetic lenses. 
C)  TEM uses a projector lens while SEM uses a photomultiplier. 
D)  TEM projects a 2-dimensional cross section of a microbe while SEM projects a 3-dimensional image with surface details. 
A)  TEM uses light waves and electrons while SEM uses only electron beams. 
B)  TEM uses electromagnetic condenser lens as well as an electromagnetic objective lens while SEM uses only 2 electromagnetic lenses. 
C)  TEM uses a projector lens while SEM uses a photomultiplier. 
D)  TEM projects a 2-dimensional cross section of a microbe while SEM projects a 3-dimensional image with surface details.

Exam 4: Microscopy

The image below represents a(n): ________.

What type of microscope is shown below?

What of the following is a correct statement about the major differences between electron and light microscopes? (Select all that apply)

The bright field light micrograph below is an image of: ________.

The ________ holds the specimen in place on a light microscope.

Negative staining: ________. (Select all that apply)

Positive Staining technique: ________.

The very complex microscopes used in research are classified according to ________.

What are the advantages of Dark-field microscopy? (Select all that apply)

What is the effect of using immersion oil plus oil immersion objective? (Select all that apply)

Using the information from the image below, determine the magnification of the microbe.

Flagella staining requires ________ to make the flagella more visible.

Which of the following best defines the term "resolution"?

What is the correct order of microscope parts from the base to the ocular?

Which of the following statements is TRUE concerning Direct Fluorescent Antibody (DFA) Assay use in microscopy? (Select all that apply)

What is the correct order of steps in smear preparation? 1) Apply suspension of cells on slide 2) spread over surface of slide 3) heat fix or chemical fix specimen on slide 4) air dry

What dyes are used most often with fluorescence microscopy?

Differential Interference Contrast Microscopy (DIC) makes use of these features to enable microbiologists to view a living specimen: ________.

Which of the following statements about Atomic Force Microscopy is true? (Select all that apply)

What is the major difference between TEM and SEM?

Microbial World

An Introduction to the Chemical Basis of Life

The Biochemistry of Macromolecules

Prokaryote Organisms

Eukaryotic Cells

Eukaryotic Organisms

Viruses and Infectious Particles

Metabolism

Microbial Genetics and Genetic Engineering

Microbial Growth and Control

Antimicrobial Agents

Innate Immunity

Adaptive Immunity

Vaccination, Immunoassays, and Immune Disorders

Microbial Pathogenesis

Epidemiology and Infection Control

Diseases of the Respiratory System

Diseases of the Skin and Eyes

Diseases of the Gastrointestinal System

Diseases of the Urogenital System

Diseases of the Nervous System

Diseases of the Cardiovascular and Lymphatic Systems

Environmental and Industrial Microbiology

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