Question 1

What is the incorrect match below?

Accepted Answer

A)  Light microscope/visible light and glass lenses. 
B)  Electron microscope/electron beam and electromagnetic lenses. 
C)  Nanoprobe microscope/ fine-tipped probes are used to penetrate the surface of a specimen to magnify internal structures. 
D)  Confocal microscope/illuminates one focal plane at a time which is then reassembled into a three-dimensional image. 
A)  Light microscope/visible light and glass lenses. 
B)  Electron microscope/electron beam and electromagnetic lenses. 
C)  Nanoprobe microscope/ fine-tipped probes are used to penetrate the surface of a specimen to magnify internal structures. 
D)  Confocal microscope/illuminates one focal plane at a time which is then reassembled into a three-dimensional image.

Question 2

A bright field microscope ________. (Select all that apply)

Accepted Answer

A)  produces an image showing fluorescent portions of specimen 
B)  is the simplest, most common type of microscope 
C)  produces a bright image against dark background 
D)  produces the image of a stained specimen is visualized against a bright background 
A)  produces an image showing fluorescent portions of specimen 
B)  is the simplest, most common type of microscope 
C)  produces a bright image against dark background 
D)  produces the image of a stained specimen is visualized against a bright background

Question 3

A dark field microscope ________.

Accepted Answer

A)  produces an image showing fluorescent portions of specimen 
B)  is the simplest, most common type of microscope 
C)  produces a bright image against dark background 
D)  produces the image of a stained specimen is visualized against a bright background 
A)  produces an image showing fluorescent portions of specimen 
B)  is the simplest, most common type of microscope 
C)  produces a bright image against dark background 
D)  produces the image of a stained specimen is visualized against a bright background

Question 4

What is the primary stain used in the Gram stain?

Accepted Answer

A)  Crystal violet 
B)  Safranin 
C)  Malachite Green 
D)  Methylene Blue 
A)  Crystal violet 
B)  Safranin 
C)  Malachite Green 
D)  Methylene Blue

Question 5

What two features of a microscope are essential for a microbiologist?

Accepted Answer

A)  Magnification and resolution 
B)  Magnification and oil immersion 
C)  Resolution and oil immersion 
D)  Objectives and oculars 
A)  Magnification and resolution 
B)  Magnification and oil immersion 
C)  Resolution and oil immersion 
D)  Objectives and oculars

Question 6

What type of microscope was used to produce the image below?

Accepted Answer

A)  Dark field microscope 
B)  Bright field microscope 
C)  Fluorescence microscope 
D)  Phase contrast microscope 
A)  Dark field microscope 
B)  Bright field microscope 
C)  Fluorescence microscope 
D)  Phase contrast microscope

Question 7

Among the characteristics of Positive Staining are: ________. (Select all that apply)

Accepted Answer

A)  cells are colored against a clear background 
B)  The positive charge of stain is attracted to negatively charges on DNA and surface proteins 
C)  examples include nigrosin and India ink 
D)  Examples include methylene blue and crystal violet 
A)  cells are colored against a clear background 
B)  The positive charge of stain is attracted to negatively charges on DNA and surface proteins 
C)  examples include nigrosin and India ink 
D)  Examples include methylene blue and crystal violet

Question 8

A 20-year-old male presented at the emergency room with intense burning during urination. A copious amount of pus was being discharged from his penis. A micrograph of a gram stain of the pus is shown below. The man is infected with ________.

Accepted Answer

A)  gram negative bacteria that have infected leukocytes 
B)  gram positive bacteria that have infected leukocytes 
C)  gram positive bacteria and gram positive eukaryotic pathogens 
D)  gram negative bacteria and gram negative eukaryotic pathogens 
A)  gram negative bacteria that have infected leukocytes 
B)  gram positive bacteria that have infected leukocytes 
C)  gram positive bacteria and gram positive eukaryotic pathogens 
D)  gram negative bacteria and gram negative eukaryotic pathogens

Question 9

Which of the following is a major characteristic of super-resolution microscopy? (Select all that apply)

Accepted Answer

A)  It increases the resolution of transmission electron microscopy 
B)  It increases the resolution limit of a confocal microscope 
C)  It is able to produce an image in very low light 
D)  It can visualize living and dead cells 
A)  It increases the resolution of transmission electron microscopy 
B)  It increases the resolution limit of a confocal microscope 
C)  It is able to produce an image in very low light 
D)  It can visualize living and dead cells

Question 10

The light micrograph below is an example of: ________.

Accepted Answer

A)  A capsule stain 
B)  An acid fast stain 
C)  An endospore stain 
D)  Dark field microscopy 
A)  A capsule stain 
B)  An acid fast stain 
C)  An endospore stain 
D)  Dark field microscopy

Question 11

What is the primary stain used in the Acid-Fast stain?

Accepted Answer

A)  Crystal violet 
B)  Carbol fuchsin 
C)  Malachite Green 
D)  Methylene Blue 
A)  Crystal violet 
B)  Carbol fuchsin 
C)  Malachite Green 
D)  Methylene Blue

Question 12

The________ magnify the specimen on a light microscope.

Accepted Answer

A)  Iris diaphragm 
B)  Condenser lens 
C)  Objective and Ocular lenses 
D)  Stage 
A)  Iris diaphragm 
B)  Condenser lens 
C)  Objective and Ocular lenses 
D)  Stage

Question 13

The condenser ________.

Accepted Answer

A)  focuses light on specimen 
B)  separately magnify specimen 
C)  holds the specimen in place 
D)  controls light intensity via adjustable opening 
A)  focuses light on specimen 
B)  separately magnify specimen 
C)  holds the specimen in place 
D)  controls light intensity via adjustable opening

What is the incorrect match below?

A bright field microscope ________. (Select all that apply)

A dark field microscope ________.

What is the primary stain used in the Gram stain?

What two features of a microscope are essential for a microbiologist?

What type of microscope was used to produce the image below?

Among the characteristics of Positive Staining are: ________. (Select all that apply)

A 20-year-old male presented at the emergency room with intense burning during urination. A copious amount of pus was being discharged from his penis. A micrograph of a gram stain of the pus is shown below. The man is infected with ________.

Which of the following is a major characteristic of super-resolution microscopy? (Select all that apply)

The light micrograph below is an example of: ________.

What is the primary stain used in the Acid-Fast stain?

The________ magnify the specimen on a light microscope.

The condenser ________.

Microbial World

An Introduction to the Chemical Basis of Life

The Biochemistry of Macromolecules

Prokaryote Organisms

Eukaryotic Cells

Eukaryotic Organisms

Viruses and Infectious Particles

Metabolism

Microbial Genetics and Genetic Engineering

Microbial Growth and Control

Antimicrobial Agents

Innate Immunity

Adaptive Immunity

Vaccination, Immunoassays, and Immune Disorders

Microbial Pathogenesis

Epidemiology and Infection Control

Diseases of the Respiratory System

Diseases of the Skin and Eyes

Diseases of the Gastrointestinal System

Diseases of the Urogenital System

Diseases of the Nervous System

Diseases of the Cardiovascular and Lymphatic Systems

Environmental and Industrial Microbiology

Filters

Exam 4: Microscopy

What is the incorrect match below?

A bright field microscope ________. (Select all that apply)

A dark field microscope ________.

What is the primary stain used in the Gram stain?

What two features of a microscope are essential for a microbiologist?

What type of microscope was used to produce the image below?

Among the characteristics of Positive Staining are: ________. (Select all that apply)

A 20-year-old male presented at the emergency room with intense burning during urination. A copious amount of pus was being discharged from his penis. A micrograph of a gram stain of the pus is shown below. The man is infected with ________.

Which of the following is a major characteristic of super-resolution microscopy? (Select all that apply)

The light micrograph below is an example of: ________.

What is the primary stain used in the Acid-Fast stain?

The________ magnify the specimen on a light microscope.

The condenser ________.

Microbial World

An Introduction to the Chemical Basis of Life

The Biochemistry of Macromolecules

Prokaryote Organisms

Eukaryotic Cells

Eukaryotic Organisms

Viruses and Infectious Particles

Metabolism

Microbial Genetics and Genetic Engineering

Microbial Growth and Control

Antimicrobial Agents

Innate Immunity

Adaptive Immunity

Vaccination, Immunoassays, and Immune Disorders

Microbial Pathogenesis

Epidemiology and Infection Control

Diseases of the Respiratory System

Diseases of the Skin and Eyes

Diseases of the Gastrointestinal System

Diseases of the Urogenital System

Diseases of the Nervous System

Diseases of the Cardiovascular and Lymphatic Systems

Environmental and Industrial Microbiology

Filters