Question 1

Confocal Microscopy: ________.

Accepted Answer

A)  Uses an electrically charged nanoprobe to scan the surface of a metal-coated specimen 
B)  Produces a three-dimensional image by assembling scanned regions of a fluorescent specimen 
C)  Displays unstained cellular structures that are visualized using differences in refractive indices 
D)  An ultrathin specimen is penetrated by an electron beam to show very good magnification and detailed resolution 
A)  Uses an electrically charged nanoprobe to scan the surface of a metal-coated specimen 
B)  Produces a three-dimensional image by assembling scanned regions of a fluorescent specimen 
C)  Displays unstained cellular structures that are visualized using differences in refractive indices 
D)  An ultrathin specimen is penetrated by an electron beam to show very good magnification and detailed resolution

Question 2

What type of microscope was used to produce the image below?

Accepted Answer

A)  Differential interference contrast microscopy 
B)  Bright field microscope 
C)  Fluorescence microscope 
D)  Confocal microscope 
A)  Differential interference contrast microscopy 
B)  Bright field microscope 
C)  Fluorescence microscope 
D)  Confocal microscope

Question 3

Which of the following statements about Scanning Tunneling Microscopy is correct? (Select all that apply)

Accepted Answer

A)  It utilizes an electrically charged nanoprobe to scan surface of a metal-coated specimen. 
B)  It measures changes in voltage detected by the nanoprobe. 
C)  The voltage changes detected by the probe are converted to an image. 
D)  The resolution is not good enough to see anything at the atomic level. 
A)  It utilizes an electrically charged nanoprobe to scan surface of a metal-coated specimen. 
B)  It measures changes in voltage detected by the nanoprobe. 
C)  The voltage changes detected by the probe are converted to an image. 
D)  The resolution is not good enough to see anything at the atomic level.

Question 4

What is the definition of the term magnification?

Accepted Answer

A)  Ratio of the size of the bacterium to the size of the object. 
B)  Ratio of the size of the protozoan to the size of the objective. 
C)  Ratio of the size of the image to the size of the object. 
D)  Ratio of the size of the objective to the size of the microorganism. 
A)  Ratio of the size of the bacterium to the size of the object. 
B)  Ratio of the size of the protozoan to the size of the objective. 
C)  Ratio of the size of the image to the size of the object. 
D)  Ratio of the size of the objective to the size of the microorganism.

Question 5

Differential staining requires: ________. (Select all that apply)

Accepted Answer

A)  preparation of a smear 
B)  application of 2 or more different positive stains 
C)  decolorizing chemical 
D)  preparation of a hanging drop 
A)  preparation of a smear 
B)  application of 2 or more different positive stains 
C)  decolorizing chemical 
D)  preparation of a hanging drop

Question 6

Simple Stains have this characteristic: ________. (Select all that apply)

Accepted Answer

A)  Only one dye is used 
B)  Staining will show size, shape and arrangement of cells 
C)  They use a decolorizer 
D)  they use a secondary stain 
A)  Only one dye is used 
B)  Staining will show size, shape and arrangement of cells 
C)  They use a decolorizer 
D)  they use a secondary stain

Question 7

What are the major characteristics of phase contrast microscopy? (Select all that apply)

Accepted Answer

A)  The true structure of living specimens may be viewed without the potential distortion of staining. 
B)  It converts differences in refractive indices to differences in color via use of prisms. 
C)  Special filters convert differences in light that are out of phase with waves through background into differences in light intensity. 
D)  It increases the resolution limit of a confocal microscope. 
A)  The true structure of living specimens may be viewed without the potential distortion of staining. 
B)  It converts differences in refractive indices to differences in color via use of prisms. 
C)  Special filters convert differences in light that are out of phase with waves through background into differences in light intensity. 
D)  It increases the resolution limit of a confocal microscope.

Question 8

What type of microscope is shown below?

Accepted Answer

A)  Bright field microscope 
B)  Transmission electron microscope 
C)  Dark field microscope 
D)  Fluorescence microscope 
A)  Bright field microscope 
B)  Transmission electron microscope 
C)  Dark field microscope 
D)  Fluorescence microscope

Question 9

What is the primary stain used in the endospore stain?

Accepted Answer

A)  Safranin 
B)  Carbol fuchsin 
C)  Malachite Green 
D)  Methylene Blue 
A)  Safranin 
B)  Carbol fuchsin 
C)  Malachite Green 
D)  Methylene Blue

Question 10

What is the counter or secondary stain used in the Acid-Fast stain?

Accepted Answer

A)  Crystal violet 
B)  Safranin 
C)  Carbol fuchsin 
D)  Methylene Blue 
A)  Crystal violet 
B)  Safranin 
C)  Carbol fuchsin 
D)  Methylene Blue

Question 11

Capsule staining shows ________ capsules surrounding ________ cells against a ________ background.

Accepted Answer

A)  clear; colored; colored 
B)  colored; clear; colored 
C)  colored; colored; clear 
D)  clear; colored; clear 
A)  clear; colored; colored 
B)  colored; clear; colored 
C)  colored; colored; clear 
D)  clear; colored; clear

Question 12

The light micrograph below is an example of: ________.

Accepted Answer

A)  A capsule stain 
B)  An acid fast stain 
C)  An endospore stain 
D)  A Gram stain 
A)  A capsule stain 
B)  An acid fast stain 
C)  An endospore stain 
D)  A Gram stain

Question 13

The iris diaphragm ________.

Accepted Answer

A)  focuses light on specimen 
B)  separately magnify specimen 
C)  holds the specimen in place 
D)  controls light intensity via adjustable opening 
A)  focuses light on specimen 
B)  separately magnify specimen 
C)  holds the specimen in place 
D)  controls light intensity via adjustable opening

Question 14

What type of microscope is shown below?

Accepted Answer

A)  Dark field microscope 
B)  Transmission electron microscope 
C)  Scanning electron microscope 
D)  Fluorescence microscope 
A)  Dark field microscope 
B)  Transmission electron microscope 
C)  Scanning electron microscope 
D)  Fluorescence microscope

Question 15

The bright field light micrograph below is an image of: ________.

Accepted Answer

A)  gram positive bacterial cells 
B)  gram negative bacterial cells 
C)  acid fast bacterial cells 
D)  bacterial cells with endospores 
A)  gram positive bacterial cells 
B)  gram negative bacterial cells 
C)  acid fast bacterial cells 
D)  bacterial cells with endospores

Question 16

Phase Contrast Microscopy: ________.

Accepted Answer

A)  Uses an electrically charged nanoprobe to scan the surface of a metal-coated specimen 
B)  Produces a three-dimensional image by assembling scanned regions of a fluorescent specimen 
C)  Displays unstained cellular structures that are visualized using differences in refractive indices 
D)  An ultrathin specimen is penetrated by an electron beam to show very good magnification and detailed resolution 
A)  Uses an electrically charged nanoprobe to scan the surface of a metal-coated specimen 
B)  Produces a three-dimensional image by assembling scanned regions of a fluorescent specimen 
C)  Displays unstained cellular structures that are visualized using differences in refractive indices 
D)  An ultrathin specimen is penetrated by an electron beam to show very good magnification and detailed resolution

Question 17

What are the disadvantages of Bright-field microscopy? (Select all that apply)

Accepted Answer

A)  Staining is required to make specimens visible 
B)  The limit for magnification is 1000X 
C)  Images of very small specimens show very little detail, just the overall organism structure 
D)  It is the simplest, most common type of microscope 
A)  Staining is required to make specimens visible 
B)  The limit for magnification is 1000X 
C)  Images of very small specimens show very little detail, just the overall organism structure 
D)  It is the simplest, most common type of microscope

Question 18

An ultrathin specimen is penetrated by transmission of an electron beam to produce and image with high magnification and detailed resolution.

Accepted Answer

A)  Confocal Microscopy 
B)  Phase Contrast Microscopy 
C)  Transmission Electron Microscopy 
D)  Scanning Electron Microscopy 
A)  Confocal Microscopy 
B)  Phase Contrast Microscopy 
C)  Transmission Electron Microscopy 
D)  Scanning Electron Microscopy

Question 19

Electrons are scattered from the surface of a metal-coated specimen to generate a three-dimensional image with high magnification and detailed resolution.

Accepted Answer

A)  Confocal Microscopy 
B)  Phase Contrast Microscopy 
C)  Transmission Electron Microscopy 
D)  Scanning Electron Microscopy 
A)  Confocal Microscopy 
B)  Phase Contrast Microscopy 
C)  Transmission Electron Microscopy 
D)  Scanning Electron Microscopy

Question 20

In the history of microscopy, who is considered the "Father of Microbiology"?

Accepted Answer

A)  Hans Jansen 
B)  Zacharias Jansen 
C)  Antonie van Leeuwenhoek 
D)  Ernst Ruska 
A)  Hans Jansen 
B)  Zacharias Jansen 
C)  Antonie van Leeuwenhoek 
D)  Ernst Ruska

Exam 4: Microscopy

Confocal Microscopy: ________.

What type of microscope was used to produce the image below?

Which of the following statements about Scanning Tunneling Microscopy is correct? (Select all that apply)

What is the definition of the term magnification?

Differential staining requires: ________. (Select all that apply)

Simple Stains have this characteristic: ________. (Select all that apply)

What are the major characteristics of phase contrast microscopy? (Select all that apply)

What type of microscope is shown below?

What is the primary stain used in the endospore stain?

What is the counter or secondary stain used in the Acid-Fast stain?

Capsule staining shows ________ capsules surrounding ________ cells against a ________ background.

The light micrograph below is an example of: ________.

The iris diaphragm ________.

What type of microscope is shown below?

The bright field light micrograph below is an image of: ________.

Phase Contrast Microscopy: ________.

What are the disadvantages of Bright-field microscopy? (Select all that apply)

An ultrathin specimen is penetrated by transmission of an electron beam to produce and image with high magnification and detailed resolution.

Electrons are scattered from the surface of a metal-coated specimen to generate a three-dimensional image with high magnification and detailed resolution.

In the history of microscopy, who is considered the "Father of Microbiology"?

Microbial World

An Introduction to the Chemical Basis of Life

The Biochemistry of Macromolecules

Prokaryote Organisms

Eukaryotic Cells

Eukaryotic Organisms

Viruses and Infectious Particles

Metabolism

Microbial Genetics and Genetic Engineering

Microbial Growth and Control

Antimicrobial Agents

Innate Immunity

Adaptive Immunity

Vaccination, Immunoassays, and Immune Disorders

Microbial Pathogenesis

Epidemiology and Infection Control

Diseases of the Respiratory System

Diseases of the Skin and Eyes

Diseases of the Gastrointestinal System

Diseases of the Urogenital System

Diseases of the Nervous System

Diseases of the Cardiovascular and Lymphatic Systems

Environmental and Industrial Microbiology

Filters

Capsule staining shows capsules surrounding cells against a background.