Exam 4: Fundamentals of Molecular Biology

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Consider Mendel's experiments on the genetics of peas. What is the probability that two green parent pea plants would also have green offspring?

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What is the complementary sequence to the following bases 5?-TCAAGG-3?

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DNA was identified as the genetic material in experiments done by

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Gregor Mendel discovered the principles of genetics in his early experiments on

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Embryonic stem cells introduced into early mouse embryos can give rise to cells in all of the tissues of the mouse, including germ cells.

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Most eukaryotic cells transfected with DNA using liposomes will only express the introduced genes for several days because

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In the study of molecular biology one frequently hears references to the "central dogma" of molecular biology. What is meant by this term?

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The first enzymatic step in making a cDNA uses

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A common method of DNA sequencing is based on the premature termination of DNA synthesis that accompanies the use of dideoxynucleotides in the PCR reactions. Why does elongation of a growing strand cease when a dideoxynucleotide is incorporated?

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The CRISPR/Cas system has revolutionized the efficiency with which scientists can mutate a gene in vivo to investigate the functional role of the protein it encodes. Which component is not essential in the CRISPR/Cas system?

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Taq polymerase is used in the polymerase chain reaction because it

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Reverse transcriptase is an enzyme that catalyzes _______ synthesis.

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Proteins are synthesized from RNA templates through a process called _______.

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Consider this statement: An increase in the levels of a protein within a cell indicates that expression of the gene encoding it was also increased. Why is the statement not necessarily true?

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Eukaryotic genes expressed in bacteria produce products that differ from the eukaryotic protein in that they do not have _______ modification.

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SDS-polyacrylamide gel electrophoresis (SDS-PAGE) separates proteins by

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The DNA made from an mRNA is called _______.

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Which technique is used to inactivate a gene by altering the DNA that encodes it?

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In addition to mRNA, the two types of RNA necessary for protein synthesis are _______ and _______.

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To clone genes, a plasmid vector should contain all of the following except

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