Exam 4: Fundamentals of Molecular Biology

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Our fundamental knowledge about mutation, genetic linkage, and the relationships between genes and chromosomes was acquired largely from experiments on the

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A single species of antibodies produced from a cultured clone of B lymphocytes is called a(n) _______.

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Genes that do not normally separate from one another during meiosis are on the same chromosome.

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The central dogma of molecular biology is _______ \rightarrow _______ \rightarrow _______.

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Which pair of primers will amplify the template DNA sequence shown below in a PCR? 5ʹ-AGGCTGCGAT...//...CCGTTAGATT-3ʹ 3ʹ-TCCGACGCTA...//...GGCAATCTAA-5ʹ

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Erwin Chargaff found that in DNA, the amount of A is equal to that of T and the amount of G is equal to that of

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EcoRI and NotI are two DNA restriction enzymes that recognize the specific sequences GAATTC and GCGGCCGC, respectively. Which of these restriction enzymes would cleave DNA most frequently, and why?

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Avery, MacLeod, and McCarty showed that one type of bacteria could be transformed into another type by uptake of purified RNA.

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A PCR reaction does not require

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Which statement correctly represents the distinction between the terms "genotype" and "phenotype"?

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Retroviral vectors can be advantageous over liposomes for stable introduction of DNA into animal cells because

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A schematic representation of a length of DNA showing the locations of cleavage sites for multiple different restriction endonucleases is called a _______.

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If adenine makes up 22% of the bases in a given double-stranded DNA molecule, what percentage is made up of guanines?

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The characterization of restriction endonucleases was a key step in the development of recombinant DNA technology. What is the function of these enzymes?

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The experimental introduction of DNA into eukaryotic cells is called

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CRISPR were discovered in bacteria, where they are used as a defense against foreign genetic elements, such as _______.

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In Gregor Mendel's experiments on pea genetics, he determined that yellow seed color, Y, was dominant over green seed color, y. In a cross between two plants, one with yellow seeds and the other with green seeds, all the seeds produced from the offspring were yellow. What was the genotype of the parent plant with yellow seeds?

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You have identified an unknown protein of approximately 1,500 amino acids. The minimum length of its mRNA is approximately

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Transgenic plants are easier to produce than transgenic animals because

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Site-specific mutations can be introduced into cultured animal cells by a technique that relies on _______ recombination.

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