Question 1

DNA is a double helix with _______ base pairs per complete turn of the helix.

Accepted Answer

A)  3.4 
B)  10 
C)  20 
D)  34 
A)  3.4 
B)  10 
C)  20 
D)  34

Question 2

Vectors usually contain an antibiotic-resistance gene so that cells that incorporate the vector can be selected by their resistance to the antibiotic.

Accepted Answer

A) True 
 B)False

Question 3

In RNA interference, expression of a gene is most effectively inhibited by injection of

Accepted Answer

A)  antisense RNA complementary to its mRNA. 
B)  sense RNA complementary to its mRNA. 
C)  double-stranded DNA. 
D)  double-stranded RNA. 
A)  antisense RNA complementary to its mRNA. 
B)  sense RNA complementary to its mRNA. 
C)  double-stranded DNA. 
D)  double-stranded RNA.

Question 4

Because the genetic code has more than one codon for most amino acids, the code is said to be

Accepted Answer

A)  redundant. 
B)  wobbly. 
C)  a template. 
D)  degenerate. 
A)  redundant. 
B)  wobbly. 
C)  a template. 
D)  degenerate.

Question 5

The two strands of DNA in the double helix are held together by

Accepted Answer

A)  interactions between the sugar residues of each chain. 
B)  interactions between phosphate residues of each strand. 
C)  hydrogen bonds between the bases of each strand. 
D)  covalent bonds between the bases of each strand. 
A)  interactions between the sugar residues of each chain. 
B)  interactions between phosphate residues of each strand. 
C)  hydrogen bonds between the bases of each strand. 
D)  covalent bonds between the bases of each strand.

Question 6

When introduced into living cells, antisense RNA molecules bind

Accepted Answer

A)  to complementary DNA sequences and block RNA synthesis. 
B)  to the sense strand of DNA and block RNA synthesis. 
C)  to complementary mRNA sequences and block protein synthesis. 
D)  proteins called RISC and cleave the complementary mRNA. 
A)  to complementary DNA sequences and block RNA synthesis. 
B)  to the sense strand of DNA and block RNA synthesis. 
C)  to complementary mRNA sequences and block protein synthesis. 
D)  proteins called RISC and cleave the complementary mRNA.

Question 7

How many different RNA triplets specify amino acids?

Accepted Answer

A)  36 
B)  60 
C)  61 
D)  64 
A)  36 
B)  60 
C)  61 
D)  64

Question 8

The technique by which a particular nucleic acid sequence is localized with labeled probes within a cell, tissue, or isolated chromosome is called _______ hybridization.

Accepted Answer

A)  in vivo 
B)  in vitro 
C)  in situ 
D)  in toto 
A)  in vivo 
B)  in vitro 
C)  in situ 
D)  in toto

Question 9

How many molecules would be produced from two molecules of DNA following five rounds of PCR amplification?

Accepted Answer

A)  10 
B)  32 
C)  64 
D)  One billion 
A)  10 
B)  32 
C)  64 
D)  One billion

Question 10

Which character is not necessary in a plasmid vector to facilitate successful cloning?

Accepted Answer

A)  Origin of replication 
B)  Gene conferring resistance to antibiotic 
C)  Restriction site 
D)  Strong bacterial promoter 
A)  Origin of replication 
B)  Gene conferring resistance to antibiotic 
C)  Restriction site 
D)  Strong bacterial promoter

Question 11

Considering Mendel's findings on the genetics of peas, identify the genotypes of two yellow parent plants that produced a green offspring.

Accepted Answer

Both parents would have to be

Question 12

The CRISPR/Cas system is a fast and powerful method to introduce specific mutations into cellular genomes via homologous recombination to study the function of genes and the proteins they encode. Which reagents are necessary for CRISPR/Cas?

Accepted Answer

A)  Antibody to DNA nuclease, Cas9 
B)  Mutant copy of target gene, guide RNA, and Cas9 
C)  Antisense RNA complementary to target gene, Cas9 
D)  siRNA complementary to the target mRNA, Cas9 
A)  Antibody to DNA nuclease, Cas9 
B)  Mutant copy of target gene, guide RNA, and Cas9 
C)  Antisense RNA complementary to target gene, Cas9 
D)  siRNA complementary to the target mRNA, Cas9

Question 13

Suppose you have a cell line that expresses a protein whose structure you know but not the function. Describe an experiment that would help you determine the protein's function.

Accepted Answer

There are several possible experiments.

Question 14

One method of DNA sequencing involves incorporation of dideoxynucleotides during synthesis of new DNA.

Accepted Answer

A) True 
 B)False

Question 15

Which method would be used to determine if drug treatment of a cell culture led to increased expression of mRNA?

Accepted Answer

A)  Southern blotting 
B)  Northern blotting 
C)  Western blotting 
D)  Eastern blotting 
A)  Southern blotting 
B)  Northern blotting 
C)  Western blotting 
D)  Eastern blotting

Question 16

Methods of sequencing DNA that have made it possible to sequence whole genomes faster and less expensively than the Sanger dideoxy method are collectively referred to as .

Accepted Answer

next-gener

Question 17

When foreign DNA is injected into the nucleus of a fertilized mouse egg and integrated into the mouse genome, the egg can develop into a(n) _______ mouse.

Accepted Answer

The answer of When foreign DNA is injected into the...

Question 18

Restriction endonucleases are enzymes that

Accepted Answer

A)  cleave DNA only at specific sequences. 
B)  act only on the ends of DNA strands. 
C)  act only in a single species of bacteria. 
D)  cleave only nuclear DNA. 
A)  cleave DNA only at specific sequences. 
B)  act only on the ends of DNA strands. 
C)  act only in a single species of bacteria. 
D)  cleave only nuclear DNA.

Question 19

During formation of eggs and sperm, one member of each chromosome pair is transmitted to each progeny cell in a type of cell division called

Accepted Answer

A)  mitosis. 
B)  gametogenesis. 
C)  cytokinesis. 
D)  meiosis. 
A)  mitosis. 
B)  gametogenesis. 
C)  cytokinesis. 
D)  meiosis.

Question 20

A cell that can give rise to an entire animal is called a(n) _______ cell.

Accepted Answer

The answer of A cell that can give rise to...

DNA is a double helix with _______ base pairs per complete turn of the helix.

Vectors usually contain an antibiotic-resistance gene so that cells that incorporate the vector can be selected by their resistance to the antibiotic.

In RNA interference, expression of a gene is most effectively inhibited by injection of

Because the genetic code has more than one codon for most amino acids, the code is said to be

The two strands of DNA in the double helix are held together by

When introduced into living cells, antisense RNA molecules bind

How many different RNA triplets specify amino acids?

The technique by which a particular nucleic acid sequence is localized with labeled probes within a cell, tissue, or isolated chromosome is called _______ hybridization.

How many molecules would be produced from two molecules of DNA following five rounds of PCR amplification?

Which character is not necessary in a plasmid vector to facilitate successful cloning?

Considering Mendel's findings on the genetics of peas, identify the genotypes of two yellow parent plants that produced a green offspring.

The CRISPR/Cas system is a fast and powerful method to introduce specific mutations into cellular genomes via homologous recombination to study the function of genes and the proteins they encode. Which reagents are necessary for CRISPR/Cas?

Suppose you have a cell line that expresses a protein whose structure you know but not the function. Describe an experiment that would help you determine the protein's function.

One method of DNA sequencing involves incorporation of dideoxynucleotides during synthesis of new DNA.

Which method would be used to determine if drug treatment of a cell culture led to increased expression of mRNA?

Methods of sequencing DNA that have made it possible to sequence whole genomes faster and less expensively than the Sanger dideoxy method are collectively referred to as .

When foreign DNA is injected into the nucleus of a fertilized mouse egg and integrated into the mouse genome, the egg can develop into a(n) _______ mouse.

Restriction endonucleases are enzymes that

During formation of eggs and sperm, one member of each chromosome pair is transmitted to each progeny cell in a type of cell division called

A cell that can give rise to an entire animal is called a(n) _______ cell.

An Overview of Cells and Cell Research

Molecules and Membranes

Bioenergetics and Metabolism

Genomics, Proteomics, and Systems Biology

Genes and Genomes

Replication, Maintenance, and Rearrangements of Genomic Dna

RNA Synthesis and Processing

Transcriptional Regulation and Epigenetics

Protein Synthesis, Processing, and Regulation

The Nucleus

Protein Sorting and Transport

Mitochondria, Chloroplasts, and Peroxisomes

The Cytoskeleton and Cell Movement

The Plasma Membrane

Cell Walls, the Extracellular Matrix, and Cell Interactions

Cell Signaling

The Cell Cycle

Cell Death and Cell Renewal

Cancer

Filters

Exam 4: Fundamentals of Molecular Biology

DNA is a double helix with _______ base pairs per complete turn of the helix.

Vectors usually contain an antibiotic-resistance gene so that cells that incorporate the vector can be selected by their resistance to the antibiotic.

In RNA interference, expression of a gene is most effectively inhibited by injection of

Because the genetic code has more than one codon for most amino acids, the code is said to be

The two strands of DNA in the double helix are held together by

When introduced into living cells, antisense RNA molecules bind

How many different RNA triplets specify amino acids?

The technique by which a particular nucleic acid sequence is localized with labeled probes within a cell, tissue, or isolated chromosome is called _______ hybridization.

How many molecules would be produced from two molecules of DNA following five rounds of PCR amplification?

Which character is not necessary in a plasmid vector to facilitate successful cloning?

Considering Mendel's findings on the genetics of peas, identify the genotypes of two yellow parent plants that produced a green offspring.

The CRISPR/Cas system is a fast and powerful method to introduce specific mutations into cellular genomes via homologous recombination to study the function of genes and the proteins they encode. Which reagents are necessary for CRISPR/Cas?

Suppose you have a cell line that expresses a protein whose structure you know but not the function. Describe an experiment that would help you determine the protein's function.

One method of DNA sequencing involves incorporation of dideoxynucleotides during synthesis of new DNA.

Which method would be used to determine if drug treatment of a cell culture led to increased expression of mRNA?

Methods of sequencing DNA that have made it possible to sequence whole genomes faster and less expensively than the Sanger dideoxy method are collectively referred to as .

When foreign DNA is injected into the nucleus of a fertilized mouse egg and integrated into the mouse genome, the egg can develop into a(n) _______ mouse.

Restriction endonucleases are enzymes that

During formation of eggs and sperm, one member of each chromosome pair is transmitted to each progeny cell in a type of cell division called

A cell that can give rise to an entire animal is called a(n) _______ cell.

An Overview of Cells and Cell Research

Molecules and Membranes

Bioenergetics and Metabolism

Genomics, Proteomics, and Systems Biology

Genes and Genomes

Replication, Maintenance, and Rearrangements of Genomic Dna

RNA Synthesis and Processing

Transcriptional Regulation and Epigenetics

Protein Synthesis, Processing, and Regulation

The Nucleus

Protein Sorting and Transport

Mitochondria, Chloroplasts, and Peroxisomes

The Cytoskeleton and Cell Movement

The Plasma Membrane

Cell Walls, the Extracellular Matrix, and Cell Interactions

Cell Signaling

The Cell Cycle

Cell Death and Cell Renewal

Cancer

Filters