Question 1

Mrs. Smith has the following chemistry results:
CK: moderately increased
LD: moderately increased
LD-1 : increased
The probable diagnosis is:

Accepted Answer

A)  Myocardial infarction. 
B)  Acute pancreatitis. 
C)  Acute hepatitis. 
D)  Cholecystitis. 
A)  Myocardial infarction. 
B)  Acute pancreatitis. 
C)  Acute hepatitis. 
D)  Cholecystitis. A

Question 2

A substrate used in lipase reactions is:

Accepted Answer

A)  Starch. 
B)  ?-nitrophenyl phosphate. 
C)  Olive oil emulsion. 
D)  Creatine phosphate. 
A)  Starch. 
B)  ?-nitrophenyl phosphate. 
C)  Olive oil emulsion. 
D)  Creatine phosphate. C

Question 3

Serum lipase catalyzes:

Accepted Answer

A)  Hydrolysis of triglyceride to fatty acids and glycerol. 
B)  Hydrolysis of triglyceride to fatty acids and water. 
C)  Synthesis of triglyceride from fatty acids and water. 
D)  Synthesis of triglyceride from fatty acids and glycerol. 
A)  Hydrolysis of triglyceride to fatty acids and glycerol. 
B)  Hydrolysis of triglyceride to fatty acids and water. 
C)  Synthesis of triglyceride from fatty acids and water. 
D)  Synthesis of triglyceride from fatty acids and glycerol. A

Question 4

Zero order kinetics results:

Accepted Answer

A)  When there are equal amounts of enzyme and substrate present. 
B)  When there is more enzyme than substrate present. 
C)  When all of the available sites on the enzyme are saturated with substrate. 
D)  When none of the available sites are saturated with substrate. 
A)  When there are equal amounts of enzyme and substrate present. 
B)  When there is more enzyme than substrate present. 
C)  When all of the available sites on the enzyme are saturated with substrate. 
D)  When none of the available sites are saturated with substrate.

Question 5

Which of the following would NOT show an increase in alkaline phosphatase?

Accepted Answer

A)  Obstructive liver disease (hepatobiliary) rickets 
B)  Rickets 
C)  Hemolytic anemia 
D)  Osteoporosis 
A)  Obstructive liver disease (hepatobiliary) rickets 
B)  Rickets 
C)  Hemolytic anemia 
D)  Osteoporosis

Question 6

Which of the following sets of results would be consistent with macroamylosemia?

Accepted Answer

A)  Normal serum amylase and elevated urine amylase values 
B)  Increased serum amylase and normal urine amylase values 
C)  Increased serum and urine amylase 
D)  Normal serum and urine amylase values 
A)  Normal serum amylase and elevated urine amylase values 
B)  Increased serum amylase and normal urine amylase values 
C)  Increased serum and urine amylase 
D)  Normal serum and urine amylase values

Question 7

Aspartate aminotransferase is elevated in diseases involving the:

Accepted Answer

A)  Renal cortex. 
B)  Heart muscle. 
C)  Intestinal epithelium. 
D)  Acinar cells of the pancreas. 
A)  Renal cortex. 
B)  Heart muscle. 
C)  Intestinal epithelium. 
D)  Acinar cells of the pancreas.

Question 8

Enzymes that catalyze the transfer of groups other than hydrogen between compounds are classified as belonging to which enzyme class?

Accepted Answer

A)  Hydrolases 
B)  Lyases 
C)  Oxidoreductases 
D)  Transferases 
A)  Hydrolases 
B)  Lyases 
C)  Oxidoreductases 
D)  Transferases

Question 9

In which liver disease is the DeRitis ratio (AST: ALT) usually less than or equal to 1?

Accepted Answer

A)  Acute viral hepatitis 
B)  Cirrhosis 
C)  Alcoholic hepatitis 
D)  All of the above 
A)  Acute viral hepatitis 
B)  Cirrhosis 
C)  Alcoholic hepatitis 
D)  All of the above

Question 10

Many enzymes in the blood plasma show maximum activity in which of the following pH ranges?

Accepted Answer

A)  4 to 5 
B)  6 to 7 
C)  7 to 8 
D)  8 to 9 
A)  4 to 5 
B)  6 to 7 
C)  7 to 8 
D)  8 to 9

Question 11

The most sensitive indicator of alcoholic liver disease is:

Accepted Answer

A)  AST. 
B)  ALT. 
C)  ?GT. 
D)  ALP. 
A)  AST. 
B)  ALT. 
C)  ?GT. 
D)  ALP.

Question 12

Malate dehydrogenase (MD) is used in the aspartate transferase reaction mixture to convert:

Accepted Answer

A)  L-glutamate to alpha oxoglutarate. 
B)  L-glutamate to L-aspartate. 
C)  L-malate to L-glutamate. 
D)  Oxaloacetate to L-malate. 
A)  L-glutamate to alpha oxoglutarate. 
B)  L-glutamate to L-aspartate. 
C)  L-malate to L-glutamate. 
D)  Oxaloacetate to L-malate.

Question 13

The prostatic fraction of acid phosphatase is inhibited by:

Accepted Answer

A)  Acetic acid. 
B)  Citric acid. 
C)  Sodium nitrite. 
D)  Tartrate. 
A)  Acetic acid. 
B)  Citric acid. 
C)  Sodium nitrite. 
D)  Tartrate.

Question 14

The best test for pancreatitis in the presence of mumps is:

Accepted Answer

A)  Virus isolation. 
B)  A serologic test for mumps. 
C)  A urine amylase test. 
D)  A serum lipase test. 
A)  Virus isolation. 
B)  A serologic test for mumps. 
C)  A urine amylase test. 
D)  A serum lipase test.

Question 15

A patient's CK-MB is reported as 18 ?g/L and the total CK is 560 IU/L. What is the CK relative index (CKI)?

Accepted Answer

A)  0.10% 
B)  3.2% 
C)  10.0% 
D)  30.0% 
A)  0.10% 
B)  3.2% 
C)  10.0% 
D)  30.0%

Question 16

Although a total lactate dehydrogenase (LD) determination performed alone yields little information as to the area of tissue destruction, an electrophoretic separation of LD isoenzyme may be useful. With what disorder is an increase in LD-5 and LD-4 associated?

Accepted Answer

A)  Acute hepatic disease 
B)  Acute myocardial infarction 
C)  Pulmonary infarction 
D)  Pancreatitis 
A)  Acute hepatic disease 
B)  Acute myocardial infarction 
C)  Pulmonary infarction 
D)  Pancreatitis

Question 17

Serum chemistry results on a 53-year-old female are
AST: 120 U/L (4-34U/L)
ALT: 185 U/L (5-40 U/L)
ALP: 785 U/L (<165 U/L)
?GT: 225 U/L (5-24 U/L)
Total Bilirubin: 10.8 mg/dL
Direct Bilirubin: 8.6 mg/dL
Urine bilirubin: positive
Urine urobilinogen: Normal
Fecal urobilinogen: decreased
These results are consistent with a diagnosis of:

Accepted Answer

A)  Hemolytic anemia. 
B)  Hepatitis. 
C)  Dubin-Johnson. 
D)  Biliary obstruction. 
A)  Hemolytic anemia. 
B)  Hepatitis. 
C)  Dubin-Johnson. 
D)  Biliary obstruction.

Question 18

Cholinesterase catalyzes the conversion of:

Accepted Answer

A)  Choline to lecithin. 
B)  Acetylcholine to choline and acetic acid. 
C)  Choline to NH4+ and PO4-3. 
D)  ?-glycerophosphate to glycerol and PO4-3. 
E)  ?-glycerophosphate to choline phosphate. 
A)  Choline to lecithin. 
B)  Acetylcholine to choline and acetic acid. 
C)  Choline to NH4+ and PO4-3. 
D)  ?-glycerophosphate to glycerol and PO4-3. 
E)  ?-glycerophosphate to choline phosphate.

Question 19

Levels of this enzyme are useful in the diagnosis of a genetic defect, which leads to increased apnea (difficulty breathing) after surgery using succinyl choline.

Accepted Answer

A)  Cholinesterase 
B)  Aldolase 
C)  Leucine aminopeptidase 
D)  5' nucleotidase 
E)  Acid phosphatase 
A)  Cholinesterase 
B)  Aldolase 
C)  Leucine aminopeptidase 
D)  5' nucleotidase 
E)  Acid phosphatase

Question 20

The indicator reaction (what is actually measured) in Oliver-Rosalki is:

Accepted Answer

A)  Adenylate kinase. 
B)  Pyruvate kinase. 
C)  Lactate dehydrogenase. 
D)  The reduction of NADP+. 
A)  Adenylate kinase. 
B)  Pyruvate kinase. 
C)  Lactate dehydrogenase. 
D)  The reduction of NADP+.

Exam 10: Enzymes

Mrs. Smith has the following chemistry results: CK: moderately increased LD: moderately increased LD-1 : increased The probable diagnosis is:

A substrate used in lipase reactions is:

Serum lipase catalyzes:

Zero order kinetics results:

Which of the following would NOT show an increase in alkaline phosphatase?

Which of the following sets of results would be consistent with macroamylosemia?

Aspartate aminotransferase is elevated in diseases involving the:

Enzymes that catalyze the transfer of groups other than hydrogen between compounds are classified as belonging to which enzyme class?

In which liver disease is the DeRitis ratio (AST: ALT) usually less than or equal to 1?

Many enzymes in the blood plasma show maximum activity in which of the following pH ranges?

The most sensitive indicator of alcoholic liver disease is:

Malate dehydrogenase (MD) is used in the aspartate transferase reaction mixture to convert:

The prostatic fraction of acid phosphatase is inhibited by:

The best test for pancreatitis in the presence of mumps is:

A patient's CK-MB is reported as 18 ?g/L and the total CK is 560 IU/L. What is the CK relative index (CKI)?

Although a total lactate dehydrogenase (LD) determination performed alone yields little information as to the area of tissue destruction, an electrophoretic separation of LD isoenzyme may be useful. With what disorder is an increase in LD-5 and LD-4 associated?

Cholinesterase catalyzes the conversion of:

Levels of this enzyme are useful in the diagnosis of a genetic defect, which leads to increased apnea (difficulty breathing) after surgery using succinyl choline.

The indicator reaction (what is actually measured) in Oliver-Rosalki is:

Laboratory Basics

Safety in the Clinical Chemistry Laboratory

Laboratory Statistics, Method Development and QC

Instrumentation, Laboratory Automation, and Informatics

Immunoassays

Molecular Diagnostics

Carbohydrates

Lipids

Proteins

Nonprotein Nitrogen Questions

Body Water and Electrolyte Homeostasis

Blood Gases, PH, and Acid-Base Balance

Mineral and Bone Metabolism

The Endocrine System

Gastrointestinal Function

Pancreas

Cardiac Function

Liver

Iron, Porphyrins, and Hemoglobin

Therapeutic Drug Monitoring

Toxic Substances

Nutrition and Vitamins

Tumor Markers

Geriatrics

Pediatrics

Filters