Question 1

Reagent antibodies prepared from human sources are:

Accepted Answer

A)  unsafe. 
B)  too low of potency to be effective. 
C)  polyclonal in specificity. 
D)  preferred because of their lower cost. 
A)  unsafe. 
B)  too low of potency to be effective. 
C)  polyclonal in specificity. 
D)  preferred because of their lower cost. C

Question 2

Select the test that uses IgG-sensitized red cells (check cells).

Accepted Answer

A)  Antiglobulin test 
B)  D-antigen typing 
C)  Rh-antigen typing 
D)  B-antigen detection 
A)  Antiglobulin test 
B)  D-antigen typing 
C)  Rh-antigen typing 
D)  B-antigen detection A

Question 3

Select the reagent from the list below and match it to the routine blood banking procedure.
-Reagent to identify the specificity of a red cell antibody

Accepted Answer

A) Panel cells 
B) Screening cells 
C) A1 and B cells 
D) ABO antisera 
E) Rh antisera
F)Lectins 
A) Panel cells 
B) Screening cells 
C) A1 and B cells 
D) ABO antisera 
E) Rh antisera
F)Lectins A

Question 4

Which red cells are used to screen for antibodies in donor samples?

Accepted Answer

A)  Screening cells (two vials) 
B)  Pooled screening cells 
C)  Panel cells 
D)  Screening cells (three vials) 
A)  Screening cells (two vials) 
B)  Pooled screening cells 
C)  Panel cells 
D)  Screening cells (three vials)

Question 5

An advantage of using a monoclonal antibody over a polyclonal antibody includes all of the following except:

Accepted Answer

A)  very few variations between lots. 
B)  no contaminating antibodies. 
C)  direct agglutination is usually faster. 
D)  all variations of the antigen can be detected. 
A)  very few variations between lots. 
B)  no contaminating antibodies. 
C)  direct agglutination is usually faster. 
D)  all variations of the antigen can be detected.

Question 6

Which of the following statements is true regarding high-protein anti-D reagents?

Accepted Answer

A)  They have been largely replaced with low protein monoclonal reagents. 
B)  They contain approximately 20% bovine albumin. 
C)  They may increase the possibility of a false-positive reaction, requiring the use of a control. 
D)  All of the above are true. 
A)  They have been largely replaced with low protein monoclonal reagents. 
B)  They contain approximately 20% bovine albumin. 
C)  They may increase the possibility of a false-positive reaction, requiring the use of a control. 
D)  All of the above are true.

Question 7

Select the reagent from the list below and match it to the routine blood banking procedure.
-Reagent used to determine the ABO antigenic composition of a patient's red cells

Accepted Answer

A) Panel cells 
B) Screening cells 
C) A1 and B cells 
D) ABO antisera 
E) Rh antisera
F)Lectins 
A) Panel cells 
B) Screening cells 
C) A1 and B cells 
D) ABO antisera 
E) Rh antisera
F)Lectins

Question 8

Select the reagent from the list below and match it to the routine blood banking procedure.
-Reagent derived from plants used to distinguish group A1 from group A2 red cells

Accepted Answer

A) Panel cells 
B) Screening cells 
C) A1 and B cells 
D) ABO antisera 
E) Rh antisera
F)Lectins 
A) Panel cells 
B) Screening cells 
C) A1 and B cells 
D) ABO antisera 
E) Rh antisera
F)Lectins

Question 9

Following centrifugation of the gel card, red cells are observed to be evenly dispersed throughout one of the microtubes. This reaction could be graded as a:

Accepted Answer

A)  4+. 
B)  3+. 
C)  2+. 
D)  1+. 
A)  4+. 
B)  3+. 
C)  2+. 
D)  1+.

Question 10

The evidence for reagent red cell deterioration may include which of the following?

Accepted Answer

A)  Spontaneous agglutination 
B)  Significant hemolysis 
C)  Loss of agglutination strength over time 
D)  All of the above 
A)  Spontaneous agglutination 
B)  Significant hemolysis 
C)  Loss of agglutination strength over time 
D)  All of the above

Question 11

Polyspecific antihuman globulin contains:

Accepted Answer

A)  anti-IgG. 
B)  anti-C3b and anti-C3d. 
C)  anti-IgG, and anti-C3d. 
D)  anti-IgG, and anti-IgM. 
A)  anti-IgG. 
B)  anti-C3b and anti-C3d. 
C)  anti-IgG, and anti-C3d. 
D)  anti-IgG, and anti-IgM.

Question 12

The indirect antihuman globulin test is incubated at what temperature?

Accepted Answer

A)  22° C 
B)  37° C 
C)  4° C 
D)  56° C 
A)  22° C 
B)  37° C 
C)  4° C 
D)  56° C

Question 13

Which of the following statements is true regarding IgG-sensitized red cells?

Accepted Answer

A)  They must be used to confirm a negative antiglobulin tube test. 
B)  They must be used to confirm a positive antiglobulin test. 
C)  They must be used to confirm a direct antiglobulin test that was negative with anti-C3d. 
D)  They should be used only with the indirect antiglobulin test. 
A)  They must be used to confirm a negative antiglobulin tube test. 
B)  They must be used to confirm a positive antiglobulin test. 
C)  They must be used to confirm a direct antiglobulin test that was negative with anti-C3d. 
D)  They should be used only with the indirect antiglobulin test.

Question 14

Why is incubation omitted in the direct antihuman globulin test?

Accepted Answer

A)  The direct antiglobulin test can be used in an emergency to replace the indirect test. 
B)  Incubation will cause hemolysis. 
C)  The antigen-antibody complex has already formed in vivo. 
D)  IgM antibodies are detected in the direct antiglobulin test. 
A)  The direct antiglobulin test can be used in an emergency to replace the indirect test. 
B)  Incubation will cause hemolysis. 
C)  The antigen-antibody complex has already formed in vivo. 
D)  IgM antibodies are detected in the direct antiglobulin test.

Question 15

Select the reagent from the list below and match it to the routine blood banking procedure.
-Reagent to detect the presence of red cell antibodies

Accepted Answer

A) Panel cells 
B) Screening cells 
C) A1 and B cells 
D) ABO antisera 
E) Rh antisera
F)Lectins 
A) Panel cells 
B) Screening cells 
C) A1 and B cells 
D) ABO antisera 
E) Rh antisera
F)Lectins

Question 16

Solid-phase red cell adherence used for antibody detection has an advantage over tube testing because:

Accepted Answer

A)  there is no washing involved. 
B)  incubation time is not necessary. 
C)  the endpoint is more clearly defined. 
D)  indicator cells (IgG-coated cells) are not necessary. 
A)  there is no washing involved. 
B)  incubation time is not necessary. 
C)  the endpoint is more clearly defined. 
D)  indicator cells (IgG-coated cells) are not necessary.

Question 17

Monoclonal antibodies are prepared in:

Accepted Answer

A)  vitro. 
B)  vivo. 
C)  laboratory animals. 
D)  humans. 
A)  vitro. 
B)  vivo. 
C)  laboratory animals. 
D)  humans.

Question 18

How would you interpret the results if both the anti-D reagent and the Rh control were 2+ agglutination reactions?

Accepted Answer

A)  D-positive 
B)  D-negative 
C)  Unable to determine without further testing 
D)  Depends on whether the sample was from a patient or a blood donor 
A)  D-positive 
B)  D-negative 
C)  Unable to determine without further testing 
D)  Depends on whether the sample was from a patient or a blood donor

Question 19

Select the reagent from the list below and match it to the routine blood banking procedure.
-Reagent used in the identification of ABO antibodies

Accepted Answer

A) Panel cells 
B) Screening cells 
C) A1 and B cells 
D) ABO antisera 
E) Rh antisera
F)Lectins 
A) Panel cells 
B) Screening cells 
C) A1 and B cells 
D) ABO antisera 
E) Rh antisera
F)Lectins

Question 20

The gel technology method uses a concentration of red cells that is:

Accepted Answer

A)  higher than tube techniques. 
B)  lower than tube techniques. 
C)  the same as the 3% to 5% requirement for tube testing. 
D)  variable according to the test performed. 
A)  higher than tube techniques. 
B)  lower than tube techniques. 
C)  the same as the 3% to 5% requirement for tube testing. 
D)  variable according to the test performed.

Reagent antibodies prepared from human sources are:

Select the test that uses IgG-sensitized red cells (check cells).

Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent to identify the specificity of a red cell antibody

Which red cells are used to screen for antibodies in donor samples?

An advantage of using a monoclonal antibody over a polyclonal antibody includes all of the following except:

Which of the following statements is true regarding high-protein anti-D reagents?

Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent used to determine the ABO antigenic composition of a patient's red cells

Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent derived from plants used to distinguish group A₁ from group A₂ red cells

Following centrifugation of the gel card, red cells are observed to be evenly dispersed throughout one of the microtubes. This reaction could be graded as a:

The evidence for reagent red cell deterioration may include which of the following?

Polyspecific antihuman globulin contains:

The indirect antihuman globulin test is incubated at what temperature?

Which of the following statements is true regarding IgG-sensitized red cells?

Why is incubation omitted in the direct antihuman globulin test?

Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent to detect the presence of red cell antibodies

Solid-phase red cell adherence used for antibody detection has an advantage over tube testing because:

Monoclonal antibodies are prepared in:

How would you interpret the results if both the anti-D reagent and the Rh control were 2+ agglutination reactions?

Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent used in the identification of ABO antibodies

The gel technology method uses a concentration of red cells that is:

Immunology: Basic Principles and Applications in the Blood Bank

Genetic Principles in Blood Banking

Abo and H Blood Group Systems and Secretor Status

Rh Blood Group System

Other Blood Group Systems

Antibody Detection and Identification

Compatibility Testing

Donor Selection and Phlebotomy

Testing of Donor Blood

Blood Component Preparation and Therapy

Adverse Complications of Transfusions

Hemolytic Disease of the Fetus and Newborn

Transfusion Therapy in Selected Patients

Quality Assurance and Regulation of the Blood Industry

Safety Issues in the Blood Bank

Filters

Exam 2: Blood Banking Reagents: Overview and Applications in Immunohematology

Reagent antibodies prepared from human sources are:

Select the test that uses IgG-sensitized red cells (check cells).

Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent to identify the specificity of a red cell antibody

Which red cells are used to screen for antibodies in donor samples?

An advantage of using a monoclonal antibody over a polyclonal antibody includes all of the following except:

Which of the following statements is true regarding high-protein anti-D reagents?

Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent used to determine the ABO antigenic composition of a patient's red cells

Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent derived from plants used to distinguish group A1 from group A2 red cells

Following centrifugation of the gel card, red cells are observed to be evenly dispersed throughout one of the microtubes. This reaction could be graded as a:

The evidence for reagent red cell deterioration may include which of the following?

Polyspecific antihuman globulin contains:

The indirect antihuman globulin test is incubated at what temperature?

Which of the following statements is true regarding IgG-sensitized red cells?

Why is incubation omitted in the direct antihuman globulin test?

Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent to detect the presence of red cell antibodies

Solid-phase red cell adherence used for antibody detection has an advantage over tube testing because:

Monoclonal antibodies are prepared in:

How would you interpret the results if both the anti-D reagent and the Rh control were 2+ agglutination reactions?

Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent used in the identification of ABO antibodies

The gel technology method uses a concentration of red cells that is:

Immunology: Basic Principles and Applications in the Blood Bank

Genetic Principles in Blood Banking

Abo and H Blood Group Systems and Secretor Status

Rh Blood Group System

Other Blood Group Systems

Antibody Detection and Identification

Compatibility Testing

Donor Selection and Phlebotomy

Testing of Donor Blood

Blood Component Preparation and Therapy

Adverse Complications of Transfusions

Hemolytic Disease of the Fetus and Newborn

Transfusion Therapy in Selected Patients

Quality Assurance and Regulation of the Blood Industry

Safety Issues in the Blood Bank

Filters

Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent derived from plants used to distinguish group A₁ from group A₂ red cells