Exam 7: Antibody Detection and Identification

Match the tentative interpretation of antibody screen and direct antiglobulin test (DAT) with the results given below. -Both screening cells positive 1+ at IS, DAT negative

Which of the following situations can be found in a classic case of autoimmune hemolytic anemia?

An autoadsorption may be performed to investigate underlying autoantibodies. When is this procedure acceptable?

In the process of identifying an antibody, the technologist observed 2+ reactions with 3 of the 10 cells in a panel at the immediate spin phase. These reactions disappeared following incubation at 37 ^$\circ$ C and the antihuman globulin phase of testing. The antibody most likely to be responsible is:

The next step in investigating a positive direct antiglobulin test using polyspecific antihuman globulin reagent should be to:

If an anti-I is suspected in a patient's sample that requires a transfusion, the most acceptable course of action is to:

Anti-D, anti-K, and anti-Jk^a are the antibodies that are tentatively identified on a panel after initially ruling out on negative cells. What selected cell from another panel should be chosen to confirm the presence of anti-K?

Antibodies to low-incidence antigens include all of the following except:

Proteolytic enzymes should not be used to screen for antibodies because:

Cold autoantibodies are typically of which specificity?

The phase of the agglutination reaction is important in the interpretation of the antibody screen or antibody identification panel because it:

Match the tentative interpretation of antibody screen and direct antiglobulin test (DAT) with the results given below. -Both screening cells positive 1+ at IS, DAT positive, C3 1+

An autoadsorption uses what type of cells to remove antibody from the serum?

In an antibody identification panel, only one red cell was negative at the antihuman globulin phase. On ruling out and matching the pattern, an anti-k was identified. What further testing is necessary to confirm the antibody?

The best description of the elution technique is that it is a technique used to:

All the following antigens are commonly found on screening cells except:

An anti-Fy^a was identified in a patient's serum. The patient's red cells phenotyped as Fy^a positive using commercial antisera. The next step is to:

Rh system antibodies characteristically give:

Antibody screening cells are positive at the antihuman globulin phase of testing. The first step of the investigation should be to:

An example of a cold alloantibody includes: