Exam 21: Gene Expression: Ithe Genetic Code and Transcription

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You have identified a protein that you feel is a sigma factor in E.coli that has not yet been characterized to date.Design an experiment whereby use can determine that this factor is a sigma factor.

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RNA polymerase recognizes which of the following DNA regions in transcription?

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You are interested in the novel expression of novel products that are transcribed in response to viral infection by respiratory epithelial cells.How would you go about specifically isolating mRNA in order to begin the process of answering your question? How would this differ if you were using a bacterial model?

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Which of the following enzymes is associated with "transcription factories" in the nuclease?

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Choose the item that best matches each item.
RNA polymerase
binds to promoter
5' mRNA cap
contains information in the form of codons
rho (ρ) factor
directs RNA polymerase to specific promoters
Correct Answer:
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Responses:
RNA polymerase
binds to promoter
5' mRNA cap
contains information in the form of codons
rho (ρ) factor
directs RNA polymerase to specific promoters
core RNA polymerase
7-methyl adenosine
CAP protein
autocatalytic RNA molecules
mRNA
transcribed gene, prior to processing
hnRNA
binds to Pribnow box
sigma (σ) subunit of RNA polymerase
contains information in the form of anticodons
tRNA
associated with transcription termination
ribozymes
polymerization of RNA
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The technique initially used in the identification of promoter sequences was ________.

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A ________ is a complex of RNA and protein that serves to remove introns from pre-mRNA.

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________-driven promoters are comprised of an Inr sequence and a TATA box with or without an associated BRE.

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A more contemporary technique for identification of promoter sequences is the ________ assay.

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If the genetic code were read in groups of four nonoverlapping nucleotides,using this nucleotide sequence,then the resulting amino acid sequence would be

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If you know the order of the nucleotides in a certain gene,what is the best way to determine which reading frame method is actually being used in a particular organism?

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The "central dogma" of retroviruses is most accurately described as

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In a eukaryotic cell,a gene that is initially transcribed is 1500 nucleotides in length.The mature mRNA for the product is 1200 nucleotides in length.What is the most probable reason for the discrepancy? A)a mutation that results in a shorter product B)removal of introns C)removal of exons D)mRNA breakage E)both choices A and D

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The ________ activity of RNA polymerase is sufficient for the process of RNA proofreading.

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With regard to RNA polymerase proofreading ability,which of the following is correct?

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In order to examine mRNA transcripts isolated from cells,one needs to convert an RNA template to DNA.Doing this conversion requires which of the following enzymes?

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If the genetic code were overlapping by one nucleotide,using three nucleotides to encode an amino acid,then the resulting amino acid sequence using this sequence would be

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Which of the following is not associated with mRNA processing in eukaryotes?

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Which of the following products are considered final products in the information flow within a cell?

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