Question 1

The DNA technique used to initially identity promoter sequences was

Accepted Answer

A) DNA fingerprinting. 
B) chromosomal walking. 
C) DNA footprinting. 
D) chromatin immunoprecipitation assay. 
E) RNA protection. 
A) DNA fingerprinting. 
B) chromosomal walking. 
C) DNA footprinting. 
D) chromatin immunoprecipitation assay. 
E) RNA protection.

Question 2

An agent that would work well as an antibiotic against prokaryotic infection would target the

Accepted Answer

A) 50S large ribosomal subunit. 
B) RNA polymerase II. 
C) 40S small ribosomal subunit. 
D) DNA polymerase. 
E) mitochondria. 
A) 50S large ribosomal subunit. 
B) RNA polymerase II. 
C) 40S small ribosomal subunit. 
D) DNA polymerase. 
E) mitochondria.

Question 3

A change in a nucleotide sequence that results in addition or deletion of a single nucleotide and largely changes the amino acid sequence of the resulting peptide is known as a

Accepted Answer

A) nonsense mutation. 
B) silent mutation. 
C) missense mutation. 
D) frameshift mutation. 
E) none of the above 
A) nonsense mutation. 
B) silent mutation. 
C) missense mutation. 
D) frameshift mutation. 
E) none of the above

Question 4

In the process of isolating DNA for transcription and translation,you digest the DNA with BamHI and ligate the gene into a plasmid vector.However,even though all of the controls work,no transcription is obtained using your fragment.Working backward,you note that restriction digestion has removed approximately 250 base pairs from the 5' end of the gene.It is possible that during restriction digestion

Accepted Answer

A) the termination signal was removed. 
B) the RNA polymerase became inactivated. 
C) primase could not bind. 
D) the promoter was removed. 
E) none of the above 
A) the termination signal was removed. 
B) the RNA polymerase became inactivated. 
C) primase could not bind. 
D) the promoter was removed. 
E) none of the above

Question 5

Explain how an electrophoretic mobility shift assay (EMSA)works.How might one use this technique?

Accepted Answer

The EMSA is for the electrophoretic sepa

Question 6

________-driven promoters do not contain TATA boxes or BREs.

Accepted Answer

The answer of ________-driven promoters do not contain TATA boxes...

Question 7

You expect that a mutation in E.coli that was recently isolated results in loss of enzyme R activity.In looking for the gene,you find comparable sequences.Using an in vitro transcription translation system,you use the gene obtained from the mutant,as well as a wild type E.coli as a control.Following protein isolation and gel electrophoresis,you observe that the wild type E.coli produces a protein that is 35K in size,corresponding to enzyme R.However,the mutant strain produces a protein that is 25K in size.What does this information suggest?

Accepted Answer

A) A nonsense mutation has occurred, resulting in a truncated, nonfunctional product in the mutant. 
B) A large addition has occurred in the wild type strain, such that approximately 35 tandem repeats of this gene are present and are biologically active. 
C) A missense mutation has occurred, resulting in a truncated, nonfunctional product in the mutant. 
D) all of the above 
E) Not enough information is given. 
A) A nonsense mutation has occurred, resulting in a truncated, nonfunctional product in the mutant. 
B) A large addition has occurred in the wild type strain, such that approximately 35 tandem repeats of this gene are present and are biologically active. 
C) A missense mutation has occurred, resulting in a truncated, nonfunctional product in the mutant. 
D) all of the above 
E) Not enough information is given.

Question 8

A change in a single nucleotide that results in no change in the amino acid sequence of the polypeptide produced is known as a

Accepted Answer

A) nonsense mutation. 
B) silent mutation. 
C) missense mutation. 
D) frameshift mutation. 
E) none of the above 
A) nonsense mutation. 
B) silent mutation. 
C) missense mutation. 
D) frameshift mutation. 
E) none of the above

Question 9

Of the following,which is associated with the production of multiple mRNAs from a single gene?

Accepted Answer

A) alternative splicing 
B) spliceosome mutations 
C) polycistronic organization 
D) gene rearrangement 
E) both choices B and C 
A) alternative splicing 
B) spliceosome mutations 
C) polycistronic organization 
D) gene rearrangement 
E) both choices B and C

Question 10

Transcription of ribosomal RNA is primarily the function of

Accepted Answer

A) primase. 
B) RNA polymerase I. 
C) RNA polymerase II. 
D) RNA polymerase III. 
E) mitochondrial RNA polymerase. 
A) primase. 
B) RNA polymerase I. 
C) RNA polymerase II. 
D) RNA polymerase III. 
E) mitochondrial RNA polymerase.

Question 11

A change in nucleotide sequence that results in a change to a stop codon is known as a

Accepted Answer

A) nonsense mutation. 
B) silent mutation. 
C) missense mutation. 
D) frameshift mutation. 
E) transversion mutation. 
A) nonsense mutation. 
B) silent mutation. 
C) missense mutation. 
D) frameshift mutation. 
E) transversion mutation.

Question 12

If you were designing a method to specifically inhibit prokaryotic transcription,but not eukaryotic transcription,interfering with which of the following would work best?

Accepted Answer

A) an intercalating agent 
B) recognition of the promoter region 
C) DNase activity 
D) RNA polymerase II activity 
E) ribosomal binding 
A) an intercalating agent 
B) recognition of the promoter region 
C) DNase activity 
D) RNA polymerase II activity 
E) ribosomal binding

Question 13

You are trying to discover what novel proteins are expressed by a T cell line in response to interaction with a novel antigen associated with HIV.As this is a potential vaccine candidate,you need to see what features of the immune response are initiated by T cells.How would you go about the task?

Accepted Answer

Isolate mRNA from the activated T cells.

Question 14

A mutation that results in the addition or deletion of a single nucleotide that radically changes the amino acid sequence of the resulting polypeptide is known as a ________ mutation.

Accepted Answer

The answer of A mutation that results in the addition...

Question 15

One reason that prokaryotic genes do not contain introns is that

Accepted Answer

A) the introns are cut out during binary fission. 
B) due to the short time the bacterium is around as a single organism, a 5' cap and poly (
A) tail are added immediately for translation to take place. 
C) bacterial proteins are very short and not subject to mutation. 
D) translation sometimes begins before transcription has been completed because prokaryotes do not have a nucleus. 
E) integrated viral DNA is not recognized for transcription. 
A) the introns are cut out during binary fission. 
B) due to the short time the bacterium is around as a single organism, a 5' cap and poly (
A) tail are added immediately for translation to take place. 
C) bacterial proteins are very short and not subject to mutation. 
D) translation sometimes begins before transcription has been completed because prokaryotes do not have a nucleus. 
E) integrated viral DNA is not recognized for transcription.

Question 16

Which of the following is not a reason for the importance of mRNA processing in eukaryotes?

Accepted Answer

A) longevity in the cytoplasm 
B) coupling of transcription and translation 
C) presence of a nuclear membrane 
D) turnover in the cytoplasm 
E) removal of introns 
A) longevity in the cytoplasm 
B) coupling of transcription and translation 
C) presence of a nuclear membrane 
D) turnover in the cytoplasm 
E) removal of introns

Question 17

Which of the following processes is unique to the retroviral information flow within a cell?

Accepted Answer

A) RNA → protein 
B) protein → DNA 
C) RNA → DNA 
D) DNA → RNA 
E) protein → RNA 
A) RNA → protein 
B) protein → DNA 
C) RNA → DNA 
D) DNA → RNA 
E) protein → RNA

Question 18

RNA polymerase II differs from RNA polymerase I and III by having

Accepted Answer

A) fewer amino acids at the N-terminus. 
B) additional amino acids at the N-terminus. 
C) fewer amino acids at the C-terminus. 
D) additional amino acids at the C-terminus. 
E) individual amino acids, but all three RNA polymerases are the same size. 
A) fewer amino acids at the N-terminus. 
B) additional amino acids at the N-terminus. 
C) fewer amino acids at the C-terminus. 
D) additional amino acids at the C-terminus. 
E) individual amino acids, but all three RNA polymerases are the same size.

Question 19

In eukaryotic transcription,TFIID recognizes and binds the ________ box in the DNA.

Accepted Answer

The answer of In eukaryotic transcription,TFIID recognizes and binds the...

Question 20

With respect to RNA processing,which of the following is false?

Accepted Answer

A) It leads to the production of alternative gene products. 
B) Some introns are removed by spliceosomes. 
C) Some introns are self-splicing. 
D) Prokaryotic mRNAs are polyadenylated at the 3' end. 
E) Chemical modification occurs with tRNA transcripts. 
A) It leads to the production of alternative gene products. 
B) Some introns are removed by spliceosomes. 
C) Some introns are self-splicing. 
D) Prokaryotic mRNAs are polyadenylated at the 3' end. 
E) Chemical modification occurs with tRNA transcripts.

Exam 21: Gene Expression: Ithe Genetic Code and Transcription

The DNA technique used to initially identity promoter sequences was

An agent that would work well as an antibiotic against prokaryotic infection would target the

A change in a nucleotide sequence that results in addition or deletion of a single nucleotide and largely changes the amino acid sequence of the resulting peptide is known as a

Explain how an electrophoretic mobility shift assay (EMSA)works.How might one use this technique?

________-driven promoters do not contain TATA boxes or BREs.

A change in a single nucleotide that results in no change in the amino acid sequence of the polypeptide produced is known as a

Of the following,which is associated with the production of multiple mRNAs from a single gene?

Transcription of ribosomal RNA is primarily the function of

A change in nucleotide sequence that results in a change to a stop codon is known as a

If you were designing a method to specifically inhibit prokaryotic transcription,but not eukaryotic transcription,interfering with which of the following would work best?

You are trying to discover what novel proteins are expressed by a T cell line in response to interaction with a novel antigen associated with HIV.As this is a potential vaccine candidate,you need to see what features of the immune response are initiated by T cells.How would you go about the task?

A mutation that results in the addition or deletion of a single nucleotide that radically changes the amino acid sequence of the resulting polypeptide is known as a ________ mutation.

One reason that prokaryotic genes do not contain introns is that

Which of the following is not a reason for the importance of mRNA processing in eukaryotes?

Which of the following processes is unique to the retroviral information flow within a cell?

RNA polymerase II differs from RNA polymerase I and III by having

In eukaryotic transcription,TFIID recognizes and binds the ________ box in the DNA.

With respect to RNA processing,which of the following is false?

A Preview of the Cell

The Chemistry of the Cell

The Macromolecules of the Cell

Cells and Organelles

Bioenergetics: the Flow of Energy in the Cell

Enzymes: the Catalysts of Life

Membranes: Their Structure, function, and Chemistry

Transport Across Membranes: Overcoming the Permeability Barrier

Chemotrophic Energy Metabolism: Glycolysis and Fermentation

Chemotrophic Energy Metabolism: Aerobic Respiration

Phototrophic Energy Metabolism: Photosynthesis

The Endomembrane System and Peroxisomes

Signal Transduction Mechanisms: Ielectrical and Synaptic Signaling in Neurons

Signal Transduction Mechanisms: Iimessengers and Receptors

Cytoskeletal Systems

Cellular Movement: Motility and Contractility

Beyond the Cell: Cell Adhesions, cell Junctions, and Extracellular Structures

The Structural Basis of Cellular Information: DNA, chromosomes, and the Nucleus

The Cell Cycle, DNA Replication, and Mitosis

Sexual Reproduction, meiosis, and Genetic Recombination

Gene Expression: Iiprotein Synthesis and Sorting

The Regulation of Gene Expression

Cancer Cells

Filters