Exam 6: Genetic Analysis and Mapping in Bacteria and Bacteriophage

Refer to this text to answer the following questions. Bacterial gene transfer is also used in the lab to introduce genes into organisms of different species,genera,phyla,or even kingdoms or domains.A major example is the use of a Ti plasmid of the soil bacterium Agrobacterium tumefaciens to transfer genes into dicotyledonous plants.The bacterium infects the plant root in the soil and transfers some of the Ti plasmid genes (T DNA)into the plant nucleus.Recombination may occur,and T DNA expression is controlled by plant compounds generated in response to wounding and infection. -The T DNA is generated when there is a nick that creates a primer for replication,followed by transfer of a single-stranded DNA piece that converts to a double-stranded piece in the plant nucleus.Which of these processes is Ti plasmid transfer most like?

When F⁺ conjugation occurs successfully,only one copy of the F plasmid single-stranded DNA is transferred.What else must minimally occur?

Which form of bacterial recombination requires no vector?

Refer to this text to answer the following questions. Bacterial gene transfer is also used in the lab to introduce genes into organisms of different species,genera,phyla,or even kingdoms or domains.A major example is the use of a Ti plasmid of the soil bacterium Agrobacterium tumefaciens to transfer genes into dicotyledonous plants.The bacterium infects the plant root in the soil and transfers some of the Ti plasmid genes (T DNA)into the plant nucleus.Recombination may occur,and T DNA expression is controlled by plant compounds generated in response to wounding and infection. -This method can be used to introduce a gene for an antigenic protein into an edible plant,such as a potato,to produce an edible vaccine (e.g.,for cholera).What has to be made a part of the Ti plasmid?

In a transduction experiment,what would constitute evidence of linkage?

If conjugation occurs with an F′ donor,how will the recipient be characterized genetically?

Once a phage genome has become part of the host chromosome,it is called a ________.

Prototrophs are bacteria that can grow on ________ medium.

In an E.coli conjugation experiment,the donor cell is found to be thr⁺,leu⁺,his⁺,and the F⁻ cell is thr⁻,leu⁻,and his⁻.When the mating is interrupted after 10 minutes and the recipients are plated,they are found to be leu⁺ and his⁺ but thr⁻.What can you conclude?

All three types of bacterial recombination share one characteristic.What is it?

What characterizes a bacterial cell that can undergo transformation?