Exam 8: Molecular Biology and Transcription and Rna Processing

In humans,the 30S pre-RNA transcript yields three rRNA segments following transcription and RNA cleavage.What RNA transcripts are generated by the 30S pre-RNA transcript in E.coli?

RNA polymerase is called a ________,meaning it is an intact complex with full enzymatic capacity.

A plant cell exhibits defects in transcription of transfer RNA genes.Which polymerase is likely to be mutated in this cell?

What proteins are bound to enhancers,forming a protein "bridge" that bends the DNA over the promoter to initiate transcription?

Following a DNA footprint protection assay,which banding pattern would you expect to see if you identify a potential promoter region?

The rat α-tropomyosin (α-Tm)gene produces nine different mature mRNAs proteins from a single gene using which three "alternative" mechanisms?

If bacteria are infected with a bacteriophage and briefly exposed to radioactive uracil,what results would you expect to see immediately after exposure?

Catalytically active RNAs that can activate processes such as self-splicing are called what?

Which type of RNA interacts with nuclear proteins to form a ribonucleoprotein complex responsible for intron removal?

What molecule is required for activation of rho protein?

Prokaryotes have a -10 and -35 consensus sequence in their promoter.What are the three eukaryotic promoter sequences elements (or "boxes")?

What is the consensus sequence for the Pribnow box from these sequences?

You suspect a region of DNA contains a promoter and wish to confirm that a sequence of interest binds proteins.Which experiment would you use?

Describe the three main difference bacterial and eukaryotic transcripts.

One type of RNA editing involves inserting uracils into edited mRNA with the assistance of which type of specialized RNA?

miRNA is capable of regulating protein production through which process?

You wish to prevent transcription of all three types of RNA in bacteria.How many different types of RNA polymerase would you need to inhibit?

The 5S rRNA and tRNA genes have internal promoters located downstream of the transcription start site,called ________.

Which type of research technique was used to track newly synthesized RNA within a eukaryotic cell?

Describe how band shift assays and DNA footprint protection assays can be used to identify promoter regions in DNA.