Question 1

Binding of which complex increases the ability of RNA polymerase to transcribe the lac operon?

Accepted Answer

CAP-cAMP

Question 2

Which molecule functions as the corepressor in the trp operon?

Accepted Answer

tryptophan

Question 3

Which proteins facilitate RNA polymerase binding at promoters?

Accepted Answer

activator proteins

Question 4

How does the arabinose (ara)operon accomplish both positive and negative transcriptional regulation? Describe the unique DNA structure that forms during one of these mechanisms,and include a diagram to illustrate the structure of the operon in both the presence and absence of arabinose.

Accepted Answer

@#IMG-DLM& 
The arabinose (ara)operon system is un

Question 5

The enzyme β-galactosidase catalyzes what reaction?

Accepted Answer

A) allolactose → glucose + lactose 
B) lactose → glucose + fructose 
C) lactose → galactose + glucose 
D) glucose → galactose + lactose 
E) galactose → glucose + lactose 
A) allolactose → glucose + lactose 
B) lactose → glucose + fructose 
C) lactose → galactose + glucose 
D) glucose → galactose + lactose 
E) galactose → glucose + lactose

Question 6

In the lac operon,what acts as the inducer?

Accepted Answer

A) glucose 
B) permease 
C) allolactose 
D) β-galactosidase 
E) transacetylase 
A) glucose 
B) permease 
C) allolactose 
D) β-galactosidase 
E) transacetylase

Question 7

What is the purpose of the lac operon,and under what circumstances is it activated? Describe the structure of the lac operon,including the repressor protein,structural genes,and regulatory regions of the operon.What is the role of catabolite repression in the lac operon?

Accepted Answer

The lac operon is responsible for the pr

Question 8

DNA-binding proteins exert control by binding DNA at specific sequences.Describe how DNA and protein interact at the molecular level.Are proteins able to identify specific DNA sequences,or do they bind to all regions with equal frequency? What motifs are commonly found in DNA-binding proteins?

Accepted Answer

DNA-protein interactions occur when the

Question 9

If a corepressor is inhibited,what effect would you expect to see in the operon?

Accepted Answer

Transcript

Question 10

In the presence of glucose,where is the lac repressor bound?

Accepted Answer

A) lacZ 
B) lacO 
C) lacP 
D) lacI 
E) The lac repressor is not bound to the operon. 
A) lacZ 
B) lacO 
C) lacP 
D) lacI 
E) The lac repressor is not bound to the operon.

Question 11

Which of these haploid strains produce β-galactosidase constitutively but do not produce permease?

Accepted Answer

A)  I- P+ O+ Z+ Y+ 
B)  I+ P+ O+ Z- Y- 
C)  I- P+ O+ Z-Y+ 
D)  I+ P+ O- Z+ Y+ 
E)  I- P+ O+ Z+ Y- 
A)  I- P+ O+ Z+ Y+ 
B)  I+ P+ O+ Z- Y- 
C)  I- P+ O+ Z-Y+ 
D)  I+ P+ O- Z+ Y+ 
E)  I- P+ O+ Z+ Y-

Question 12

Bacteria grown at 45°C initiate expression of which two types of proteins?

Accepted Answer

heat shock

Question 13

Changing conformation at the active site as a result of binding a substance at a different site is known as ________.

Accepted Answer

The answer of Changing conformation at the active site as...

Question 14

Which part of the lac operon is cis-dominant?

Accepted Answer

The answer of Which part of the lac operon is...

Question 15

What protein cleaves the λ repressor monomers to inactivate the repressor protein,and is activated by DNA-damaging agents?

Accepted Answer

The answer of What protein cleaves the λ repressor monomers...

Question 16

The presence of which combination of molecules would lead to active transcription of an operon?

Accepted Answer

A) activator + corepressor 
B) activator + repressor 
C) inducer + corepressor 
D) repressor + corepressor 
E) activator + inducer 
A) activator + corepressor 
B) activator + repressor 
C) inducer + corepressor 
D) repressor + corepressor 
E) activator + inducer

Question 17

Most of the regulation of gene expression in bacteria occurs at the ________ level.

Accepted Answer

The answer of Most of the regulation of gene expression...

Question 18

Expression of a(n)________ alters gene transcription in E.coli by activating transcription of specialized heat stress response genes.

Accepted Answer

alternativ

Question 19

The genetic switch controlling whether a bacterium enters the lytic or lysogenic cycle relies on the binding of which two proteins?

Accepted Answer

The answer of The genetic switch controlling whether a bacterium...

Question 20

Which protein in the arabinose operon is responsible for both positive and negative regulation of transcription?

Accepted Answer

The answer of Which protein in the arabinose operon is...

Exam 14: Regulation of Gene Expression in Bacteria and Bacteriophage

Binding of which complex increases the ability of RNA polymerase to transcribe the lac operon?

Which molecule functions as the corepressor in the trp operon?

Which proteins facilitate RNA polymerase binding at promoters?

How does the arabinose (ara)operon accomplish both positive and negative transcriptional regulation? Describe the unique DNA structure that forms during one of these mechanisms,and include a diagram to illustrate the structure of the operon in both the presence and absence of arabinose.

The enzyme β-galactosidase catalyzes what reaction?

In the lac operon,what acts as the inducer?

What is the purpose of the lac operon,and under what circumstances is it activated? Describe the structure of the lac operon,including the repressor protein,structural genes,and regulatory regions of the operon.What is the role of catabolite repression in the lac operon?

DNA-binding proteins exert control by binding DNA at specific sequences.Describe how DNA and protein interact at the molecular level.Are proteins able to identify specific DNA sequences,or do they bind to all regions with equal frequency? What motifs are commonly found in DNA-binding proteins?

If a corepressor is inhibited,what effect would you expect to see in the operon?

In the presence of glucose,where is the lac repressor bound?

Which of these haploid strains produce β-galactosidase constitutively but do not produce permease?

Bacteria grown at 45°C initiate expression of which two types of proteins?

Changing conformation at the active site as a result of binding a substance at a different site is known as ________.

Which part of the lac operon is cis-dominant?

What protein cleaves the λ repressor monomers to inactivate the repressor protein,and is activated by DNA-damaging agents?

The presence of which combination of molecules would lead to active transcription of an operon?

Most of the regulation of gene expression in bacteria occurs at the ________ level.

Expression of a(n)________ alters gene transcription in E.coli by activating transcription of specialized heat stress response genes.

The genetic switch controlling whether a bacterium enters the lytic or lysogenic cycle relies on the binding of which two proteins?

Which protein in the arabinose operon is responsible for both positive and negative regulation of transcription?

The Molecular Basis of Heredity, Variation, and Evolution

Transmission Genetics

Cell Division and Chromosome Heredity

Gene Interaction

Genetic Linkage and Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophage

DNA Structure and Replication

Molecular Biology and Transcription and Rna Processing

The Molecular Biology of Translation

The Integration of Genetic Approaches: Understanding Sickle Cell Disease

Chromosome Structure

Gene Mutation, DNA Repair, and Homologous Recombination

Chromosome Aberrations and Transposition

Regulation of Gene Expression in Eukaryotes

Forward Genetics and Recombinant DNA Technology

Applications of Recombinant DNA Technology and Reverse Genetics

Genomics: Genetics From a Whole-Genome Perspective

Cytoplasmic Inheritance and the Evolution of Organelle Genomes

Developmental Genetics

Genetic Analysis of Quantitative Traits

Population Genetics and Evolution

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