Question 1

Eukaryotic polymerases interact with which protein that acts as the sliding clamp in eukaryotic DNA replication?

Accepted Answer

PCNA

Question 2

The major and minor grooves of DNA are features of the helix that can be attributed to this process,which involves tight packing of DNA bases in a duplex.

Accepted Answer

base stacking

Question 3

Avery,Macleod,and McCarty expanded on Griffith's experiment to prove that DNA is the hereditary molecule required for transformation.What treatment of the heat-killed SIII bacteria extract resulted in the mouse living?

Accepted Answer

A) destroying type SIII lipids and polysaccharides 
B) destroying type SIII DNA with DNase 
C) destroying type SIII RNA with RNase 
D) destroying type SIII proteins with protease 
E) the control group,in which all components are intact 
A) destroying type SIII lipids and polysaccharides 
B) destroying type SIII DNA with DNase 
C) destroying type SIII RNA with RNase 
D) destroying type SIII proteins with protease 
E) the control group,in which all components are intact B

Question 4

What type of experimental technique did Huberman and Riggs use to produce the first evidence of bidirectional replication in bacteria?

Accepted Answer

pulse-chas

Question 5

Briefly describe the Meselson and Stahl experiment that indicated that DNA replication is semiconservative.How would their results have differed if DNA replication was actually conservative in nature?

Accepted Answer

Meselson and Stahl grew bacteria in the

Question 6

What is the molecular diameter of the DNA helix (in angstroms)?

Accepted Answer

The answer of What is the molecular diameter of the...

Question 7

Many antibacterial agents and chemotherapy agents act as topoisomerase inhibitors.Why would inhibition of topoisomerase cause cell death in bacterial and eukaryotic cells?

Accepted Answer

During DNA replication,as DNA strands un

Question 8

The large,complex aggregation of proteins and enzymes that assembles at the replication fork is known as the ________.

Accepted Answer

The answer of The large,complex aggregation of proteins and enzymes...

Question 9

During DNA replication,the continuously elongated daughter strand is referred to as what?

Accepted Answer

The answer of During DNA replication,the continuously elongated daughter strand...

Question 10

What type of bond is formed between the hydroxyl group of one nucleotide and the phosphate group of an adjacent nucleotide,forming the sugar-phosphate backbone of DNA?

Accepted Answer

A) glycosidic bond 
B) phosphodiester bond 
C) ester linkage 
D) hydrogen bond 
E) ionic bond 
A) glycosidic bond 
B) phosphodiester bond 
C) ester linkage 
D) hydrogen bond 
E) ionic bond

Question 11

________ must be added to a PCR to direct the polymerase where to begin synthesis.

Accepted Answer

The answer of ________ must be added to a PCR...

Question 12

What structure in E.coli delivers primase and accessory proteins to the oriC site to synthesize the primer needed for DNA replication?

Accepted Answer

The answer of What structure in E.coli delivers primase and...

Question 13

Which functional groups have been altered in a ddNTP compared to a dNTP?

Accepted Answer

A) The ddNTPs have a 2′ OH and a 3′ H,while dNTPs have a 2′ H and a 3′ OH. 
B) The ddNTPs have a 2′ H and a 3′ H,while dNTPs have a 2′ H and a 3′ OH. 
C) The ddNTPs have a 2′ OH and a 3′ OH,while dNTPs have a 2′ H and a 3′ H. 
D) The ddNTPs have a 2′ H and a 3′ H,while dNTPs have a 2′ OH and a 3′ OH. 
E) The ddNTPs have a 2′ H and a 3′ OH,while dNTPs have a 2′ H and a 3′ H. 
A) The ddNTPs have a 2′ OH and a 3′ H,while dNTPs have a 2′ H and a 3′ OH. 
B) The ddNTPs have a 2′ H and a 3′ H,while dNTPs have a 2′ H and a 3′ OH. 
C) The ddNTPs have a 2′ OH and a 3′ OH,while dNTPs have a 2′ H and a 3′ H. 
D) The ddNTPs have a 2′ H and a 3′ H,while dNTPs have a 2′ OH and a 3′ OH. 
E) The ddNTPs have a 2′ H and a 3′ OH,while dNTPs have a 2′ H and a 3′ H.

Question 14

What enzyme(s)is/are responsible for removal of RNA primers and joining of Okazaki fragments?

Accepted Answer

A) DNA polymerase I 
B) DNA polymerase III 
C) DNA ligase 
D) DNA polymerase I and DNA ligase 
E) DNA polymerase III and DNA ligase 
A) DNA polymerase I 
B) DNA polymerase III 
C) DNA ligase 
D) DNA polymerase I and DNA ligase 
E) DNA polymerase III and DNA ligase

Question 15

Based on Griffith's results,what would you expect if you injected both heat-killed type RII and living type SIII?

Accepted Answer

The answer of Based on Griffith's results,what would you expect...

Question 16

Comparison of conserved sequences among related species usually leads to the identification of ________,which illustrate the nucleotides most often found at each position of DNA in the conserved region.

Accepted Answer

The answer of Comparison of conserved sequences among related species...

Question 17

During DNA replication in E.coli,how does the cell prevent the DNA strands from their natural tendency to seek maximum stability and reanneal?

Accepted Answer

SSB preven

Question 18

The process by which Pneumococcus transfers DNA between living type RII and heat-killed type SIII cells is known as

Accepted Answer

A) transformation. 
B) replication. 
C) transduction. 
D) ligation. 
E) conjugation. 
A) transformation. 
B) replication. 
C) transduction. 
D) ligation. 
E) conjugation.

Question 19

In E.coli,which protein uses ATP to hydrolyze hydrogen bonds holding complementary strands together?

Accepted Answer

The answer of In E.coli,which protein uses ATP to hydrolyze...

Question 20

Describe the function of the oriC.What do you predict will happen to the cell if 100 bp between the 13-mer and 9-mer sequence is deleted as indicated in the figure?

Accepted Answer

A consensus sequence is the portion of a

Exam 7: DNA Structure and Replication

Eukaryotic polymerases interact with which protein that acts as the sliding clamp in eukaryotic DNA replication?

The major and minor grooves of DNA are features of the helix that can be attributed to this process,which involves tight packing of DNA bases in a duplex.

Avery,Macleod,and McCarty expanded on Griffith's experiment to prove that DNA is the hereditary molecule required for transformation.What treatment of the heat-killed SIII bacteria extract resulted in the mouse living?

What type of experimental technique did Huberman and Riggs use to produce the first evidence of bidirectional replication in bacteria?

Briefly describe the Meselson and Stahl experiment that indicated that DNA replication is semiconservative.How would their results have differed if DNA replication was actually conservative in nature?

What is the molecular diameter of the DNA helix (in angstroms)?

Many antibacterial agents and chemotherapy agents act as topoisomerase inhibitors.Why would inhibition of topoisomerase cause cell death in bacterial and eukaryotic cells?

The large,complex aggregation of proteins and enzymes that assembles at the replication fork is known as the ________.

During DNA replication,the continuously elongated daughter strand is referred to as what?

What type of bond is formed between the hydroxyl group of one nucleotide and the phosphate group of an adjacent nucleotide,forming the sugar-phosphate backbone of DNA?

________ must be added to a PCR to direct the polymerase where to begin synthesis.

What structure in E.coli delivers primase and accessory proteins to the oriC site to synthesize the primer needed for DNA replication?

Which functional groups have been altered in a ddNTP compared to a dNTP?

What enzyme(s)is/are responsible for removal of RNA primers and joining of Okazaki fragments?

Based on Griffith's results,what would you expect if you injected both heat-killed type RII and living type SIII?

Comparison of conserved sequences among related species usually leads to the identification of ________,which illustrate the nucleotides most often found at each position of DNA in the conserved region.

During DNA replication in E.coli,how does the cell prevent the DNA strands from their natural tendency to seek maximum stability and reanneal?

The process by which Pneumococcus transfers DNA between living type RII and heat-killed type SIII cells is known as

In E.coli,which protein uses ATP to hydrolyze hydrogen bonds holding complementary strands together?

Describe the function of the oriC.What do you predict will happen to the cell if 100 bp between the 13-mer and 9-mer sequence is deleted as indicated in the figure?

The Molecular Basis of Heredity, Variation, and Evolution

Transmission Genetics

Cell Division and Chromosome Heredity

Gene Interaction

Genetic Linkage and Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophage

Molecular Biology and Transcription and Rna Processing

The Molecular Biology of Translation

The Integration of Genetic Approaches: Understanding Sickle Cell Disease

Chromosome Structure

Gene Mutation, DNA Repair, and Homologous Recombination

Chromosome Aberrations and Transposition

Regulation of Gene Expression in Bacteria and Bacteriophage

Regulation of Gene Expression in Eukaryotes

Forward Genetics and Recombinant DNA Technology

Applications of Recombinant DNA Technology and Reverse Genetics

Genomics: Genetics From a Whole-Genome Perspective

Cytoplasmic Inheritance and the Evolution of Organelle Genomes

Developmental Genetics

Genetic Analysis of Quantitative Traits

Population Genetics and Evolution

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