Exam 8: Molecular Biology and Transcription and Rna Processing

What are the mechanisms for transcription termination in bacteria?

In a given bacterium,transcription of housekeeping genes is normal,but genes involved in nitrogen metabolism,stress,and chemotaxis are disrupted.Which sigma subunit is intact?

What proteins aid in the recognition of the promoter sequence and binding of RNA polymerase II in eukaryotes?

If you want to prevent rho-dependent transcription,which sequences would you target to mutate the rho utilization (rut)site?

Intron self-splicing occurs when two ________ reactions excise the intron and allow exons to ligate.

What proteins bind to silencer sequences,forming a protein "bridge" that bends the DNA over the promoter and prevents transcription?

Processing of the 30S pre-RNA transcript in humans produces what three rRNAs after enzymatic cleavage?

Which region(s)of a gene are not found within the mRNA transcript?

How does the antibiotic rifampicin prevent transcription of RNA?

What is the -35 consensus sequence for the following sequences?

Transcription of inverted repeats produces an mRNA with complementary segments that fold to form what type of secondary structure?

Which enzyme is required to initiate 5′ capping of eukaryotic mRNA transcripts by removing the terminal phosphate group?

Polyadenylation begins with the binding of ________ near a six-nucleotide mRNA sequence,AAUAAA,downstream of the stop codon.

A cell exhibits specific defects in the alternative intron splicing pathway.Which proteins are likely mutated in this cell?

Eukaryotic promoters are diverse,which means that eukaryotes have how many different RNA polymerases?

Nucleoli contain many tandem repeat copies of which genes,transcribed by RNA polymerase I?

In the following prokaryotic DNA sequence,label the highlighted regions. Using the prokaryotic DNA sequence above,describe the relationship between the highlighted regions and how mutations in each region may affect gene expression.

You wish to create a mutation in which you prevent access of RNA polymerase to the gene.Which region of a gene would you target?

What are the two chemical structures found in RNA but not DNA?

What are the three types of posttranscriptional processing,and what are the consequences of preventing each of these modifications in terms of gene expression?