Exam 9: The Molecular Biology of Translation

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Which of the following are synonymous codons?

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You have identified a bacterial protein that has retained the starting fMet in its protein sequence.Which of the following is likely true of this protein?

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What are the three phases of translation?

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Eighteen of the amino acids have two or more synonymous codons.Which two amino acids are the exceptions?

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In a polyribosome,which end of the mRNA would have the shortest polypeptides?

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The Shine-Dalgarno sequence

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A mutagen has introduced a frame-shift mutation by adding one nucleotide base.Which of the following would be classified as a reversion mutation for this particular mutant?

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Compare and contrast the mechanism of action of two commonly used antibiotics.Include details about which steps of translation are inhibited,and explain how the antibiotic accomplishes this inhibition.

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In the ER,misfolded proteins are identified and bound by what molecules?

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A signal sequence at the N-terminus of newly synthesized proteins will direct the protein to the ________ for protein packaging.

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Polypeptides must be sorted after translation.Does protein sorting occur in both prokaryotes and eukaryotes? Describe the process of protein sorting and explain how the signal sequence is involved.What would you expect to see if the signal sequence has been mutated or deleted?

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