Exam 10: The Integration of Genetic Approaches: Understanding Sickle Cell Disease

Which of the following techniques quantifies the amount of protein present in a gel lane by measuring how much light is blocked from passing through the gel by the presence of a band of protein?

Describe the hypothesis that James Neel proposed to describe the inheritance pattern of SCD.What experimental methods did he use to prove his hypothesis?

How is gel electrophoresis used to demonstrate the molecular basis of SCD?

Given what you know of the inheritance pattern of SCD,is the following pedigree representative of a typical SCD pedigree?

You are interested in designing a molecular probe to detect the region of βˢ SNP,which includes a DdeI (CTNAG)restriction site.Which of the following molecular probe sequences would anneal to the correct position?

Globin genes contain ________ segments that ultimately encode the amino acids of the protein and interspersed ________ sequences that do not contribute to encoding the protein product of the gene.

The βˢ and βᴬ alleles can be distinguished on the basis of

Sodium dodecyl sulfate (SDS)is a detergent commonly added to protein samples before gel electrophoresis.The purpose of the negatively charged SDS is to neutralize the charges and denature the protein to remove secondary structures.Thus,the proteins can be separated on a gel based solely on what?

Before transferring DNA from a gel to the membrane in Southern blotting,the DNA must be denatured (usually by soaking the gel in NaOH).Why is this step necessary?