Exam 12: Gene Mutation, DNA Repair, and Homologous Recombination

What is the human cancer syndrome associated with inherited p53 mutations?

Which of these statements best describes gene expression in a damaged cell?

Given the DNA sequence 5′-TAC AAA ATA CAG CGG-3′,which of these sequences represents a nonsense mutation?

If one Holliday junction region is resolved by an NS cut and the other by an EW cut,the resulting chromosomes

Which type of mutation converts a nucleotide to an alternative structure with the same composition but slightly different placement of hydrogen bonds with a rare,less stable form that causes base-pair mismatch?

Which repair mechanism is an error-prone system that uses bypass polymerases to replicate short segments of daughter strands whose normal replication is blocked by lesions in template strand DNA?

A bacterial culture is exposed to UV radiation and is preparing to repair the DNA damage.Describe two mechanisms of repair used by E.coli to repair UV-induced DNA damage.Would you expect the bacteria to be able to repair more or less of the damage if they were to be placed in a dark environment after UV damage?

What are the two basic types of base-pair substitutions?

If you have screened 100,000 pollen grains and identified 5 mutants,what is the mutation frequency?

You are interested in studying the function of a gene,so you decide to create point mutations to help you identify important residues in your protein. Which of the adenines in the following sequence could you mutate to create a silent transversion mutation? 5′-ATG GGA TGG CAC CTG-3′

DNA damage signaling processes are essential for regulating the ________ transition within the cell cycle.

A second-site mutation that compensates for the mutation in one gene by mutating a second gene and restoring the wild-type phenotype is also known as a ________ mutation.