Question 1

Which region of the lac operon would you target if you want to disrupt the -10 and -35 consensus sequences?

Accepted Answer

A) lacZ 
B) lacY 
C) lacI 
D) lacP 
E) lacO 
A) lacZ 
B) lacY 
C) lacI 
D) lacP 
E) lacO

Question 2

What is the process by which a bacterium switches from a lysogenic to lytic cycle?

Accepted Answer

The answer of What is the process by which a...

Question 3

Which mutants are noninducible?

Accepted Answer

A) lacIˢ 
B) lacI⁻ 
C) lacI⁺ 
D) lacOᶜ 
E) lacO⁺ 
A) lacIˢ 
B) lacI⁻ 
C) lacI⁺ 
D) lacOᶜ 
E) lacO⁺

Question 4

Which enzyme is responsible for integration of a temperate phage into a host genome?

Accepted Answer

A) β-galactosidase 
B) transposase 
C) integrase 
D) transacetylase 
E) permease 
A) β-galactosidase 
B) transposase 
C) integrase 
D) transacetylase 
E) permease

Question 5

Which structural gene of the lac operon can be mutated without affecting the cell's ability to break down lactose?

Accepted Answer

A) lacZ 
B) lacY 
C) lacA 
D) lacP 
E) lacO 
A) lacZ 
B) lacY 
C) lacA 
D) lacP 
E) lacO

Question 6

Which of these haploid strains produce permease but do not produce β-galactosidase?

Accepted Answer

A)  I- P+ O+ Z+ Y+ 
B)  I+ P+ O+ Z-Y+ 
C)  I- P+ O+ Z-Y- 
D)  I+ P+ O- Z+ Y+ 
E)  I- P+ O+ Z+ Y- 
A)  I- P+ O+ Z+ Y+ 
B)  I+ P+ O+ Z-Y+ 
C)  I- P+ O+ Z-Y- 
D)  I+ P+ O- Z+ Y+ 
E)  I- P+ O+ Z+ Y-

Question 7

Catabolite repression refers to the repression of the lac operon in the presence of which catabolite?

Accepted Answer

The answer of Catabolite repression refers to the repression of...

Question 8

What is the role of cis-acting and trans-acting factors in transcription? Explain how these factors play a role in the lac operon,and discuss how complementation can be used to identify where the mutations have occurred (structural genes versus regulatory regions).Which type of mutations can be complemented by a second gene with normal function? Are Iˢ and Oᶜ mutants cis or trans-acting?

Accepted Answer

If a factor is trans-acting,genes that a

Question 9

In the absence of both glucose and lactose,the presence of which molecule is capable of inducing basal transcription?

Accepted Answer

The answer of In the absence of both glucose and...

Question 10

If you wanted to prevent a cell from entering the lysogenic pathway,which gene controlled by PRM would you target?

Accepted Answer

A) cro 
B) Q 
C) cI 
D) cII 
E) O 
A) cro 
B) Q 
C) cI 
D) cII 
E) O

Which region of the lac operon would you target if you want to disrupt the -10 and -35 consensus sequences?

What is the process by which a bacterium switches from a lysogenic to lytic cycle?

Which mutants are noninducible?

Which enzyme is responsible for integration of a temperate phage into a host genome?

Which structural gene of the lac operon can be mutated without affecting the cell's ability to break down lactose?

Which of these haploid strains produce permease but do not produce β-galactosidase?

Catabolite repression refers to the repression of the lac operon in the presence of which catabolite?

In the absence of both glucose and lactose,the presence of which molecule is capable of inducing basal transcription?

If you wanted to prevent a cell from entering the lysogenic pathway,which gene controlled by PRM would you target?

The Molecular Basis of Heredity, Variation, and Evolution

Transmission Genetics

Cell Division and Chromosome Heredity

Gene Interaction

Genetic Linkage and Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophage

DNA Structure and Replication

Molecular Biology and Transcription and Rna Processing

The Molecular Biology of Translation

The Integration of Genetic Approaches: Understanding Sickle Cell Disease

Chromosome Structure

Gene Mutation, DNA Repair, and Homologous Recombination

Chromosome Aberrations and Transposition

Regulation of Gene Expression in Eukaryotes

Forward Genetics and Recombinant DNA Technology

Applications of Recombinant DNA Technology and Reverse Genetics

Genomics: Genetics From a Whole-Genome Perspective

Cytoplasmic Inheritance and the Evolution of Organelle Genomes

Developmental Genetics

Genetic Analysis of Quantitative Traits

Population Genetics and Evolution

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Exam 14: Regulation of Gene Expression in Bacteria and Bacteriophage

Which region of the lac operon would you target if you want to disrupt the -10 and -35 consensus sequences?

What is the process by which a bacterium switches from a lysogenic to lytic cycle?

Which mutants are noninducible?

Which enzyme is responsible for integration of a temperate phage into a host genome?

Which structural gene of the lac operon can be mutated without affecting the cell's ability to break down lactose?

Which of these haploid strains produce permease but do not produce β-galactosidase?

Catabolite repression refers to the repression of the lac operon in the presence of which catabolite?

In the absence of both glucose and lactose,the presence of which molecule is capable of inducing basal transcription?

If you wanted to prevent a cell from entering the lysogenic pathway,which gene controlled by PRM would you target?

The Molecular Basis of Heredity, Variation, and Evolution

Transmission Genetics

Cell Division and Chromosome Heredity

Gene Interaction

Genetic Linkage and Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophage

DNA Structure and Replication

Molecular Biology and Transcription and Rna Processing

The Molecular Biology of Translation

The Integration of Genetic Approaches: Understanding Sickle Cell Disease

Chromosome Structure

Gene Mutation, DNA Repair, and Homologous Recombination

Chromosome Aberrations and Transposition

Regulation of Gene Expression in Eukaryotes

Forward Genetics and Recombinant DNA Technology

Applications of Recombinant DNA Technology and Reverse Genetics

Genomics: Genetics From a Whole-Genome Perspective

Cytoplasmic Inheritance and the Evolution of Organelle Genomes

Developmental Genetics

Genetic Analysis of Quantitative Traits

Population Genetics and Evolution

Filters