Exam 2: Tools of the Laboratory: Methods for the Culturing and Microscopic Analysis of Microorganisms

An RN is working at an urban low-income medical clinic when a young woman enters crying. She is 19 years old and 28 weeks pregnant with her second child. The woman reports that she woke this morning to find she was leaking milky-colored fluid vaginally. Her first child was born 6 weeks early due to premature rupture of membranes and she is worried this is happening again. You reassure the patient, explain that a vaginal speculum exam will be performed, and educate her about specimens that will be collected. Once the proper specimens are obtained and appropriately labeled, the wet mount and culturette are sent to the laboratory for processing. -When utilizing a light microscope,the specimen on the glass slide must be in proper position to ensure illumination of the specimen for visualization.The glass slide is placed in which of the following positions?

Which type of microscope achieves the greatest resolution and highest magnification?

An RN is working at an urban low-income medical clinic when a young woman enters crying. She is 19 years old and 28 weeks pregnant with her second child. The woman reports that she woke this morning to find she was leaking milky-colored fluid vaginally. Her first child was born 6 weeks early due to premature rupture of membranes and she is worried this is happening again. You reassure the patient, explain that a vaginal speculum exam will be performed, and educate her about specimens that will be collected. Once the proper specimens are obtained and appropriately labeled, the wet mount and culturette are sent to the laboratory for processing. -The patient asks how microbes from her body can be grown in the lab.The RN explains that specimens are introduced to nutrient medium and that any growth of the microbe that appears after incubating the specimen is called the ________.

All of the following are examples of different types of microbiological media except ________.

Which type of microscope bombards a whole,metal-coated specimen with electrons moving back and forth over it?

What do the Gram stain,acid-fast stain,and endospore stain have in common?

Which type of microscope does not use light in forming the specimen image?

A 65-year-old homeless male presents to an urgent care clinic with a deep laceration on his left arm. He states that he cut his arm on an old piece of scrap metal two days prior. His wound is red, tender, hot to the touch, and has yellow drainage. The RN collects a sample of the drainage and sends it to the laboratory for microbial analysis, per provider orders. -The patient's culture results positive for Staphylococcus.The RN understands that the culture most likely required growth on a complex medium,consisting of 

Some microbes are not capable of growing on artificial media.

Which of the following will result when 1% to 5% agar is added to nutrient broth,boiled,and cooled?

All of the following pertain to the fluorescence microscope except ________.

A confocal scanning microscope ________.

A reducing medium contains ________.

The Five I's of studying microorganisms include all of the following except ________.

An RN is working at an urban low-income medical clinic when a young woman enters crying. She is 19 years old and 28 weeks pregnant with her second child. The woman reports that she woke this morning to find she was leaking milky-colored fluid vaginally. Her first child was born 6 weeks early due to premature rupture of membranes and she is worried this is happening again. You reassure the patient, explain that a vaginal speculum exam will be performed, and educate her about specimens that will be collected. Once the proper specimens are obtained and appropriately labeled, the wet mount and culturette are sent to the laboratory for processing. -The sterile vaginal fluid specimen is sent for culture.The RN educates the patient about the five basic techniques utilized by laboratory technicians to manipulate,grow,examine,and characterize any microorganisms present in the specimen.Which of the following is the correct order of steps for processing the specimen?

Normal incubation temperatures range from 30°C to 60°C.

Which of the following controls the amount of light entering the specimen?

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the ________ lens.This image is then projected to the ________ lens that will further magnify the specimen to form a virtual image received by the eye.

The specimen preparation that is best for viewing cell motility is ________.

An RN is working at a public health clinic that sees many patients with infectious disease. Ms. Hungh, a Burmese immigrant, presents to the clinic with an interpreter, complaining of fatigue, weight loss, persistent cough, and rust-colored sputum. The interpreter explains that Ms. Hungh has had this cough for many months in her home country and, now that she is in America, is seeking treatment for her condition. -Culturing of the sputum resulted in the growth of distinct colonies and further isolation by subculturing is now needed.The RN understands that isolation is accomplished by taking a bit of growth from an individual colony and inoculating a separate medium,resulting in the production of a(n)________.