Exam 2: Tools of the Laboratory: Methods for the Culturing and Microscopic Analysis of Microorganisms

Resolution differs from contrast in microscopy in that ________.

The Gram staining procedure is best described as a(n)________ staining technique.

In the absence of immersion oil when using the 100x objective lens,some light would be lost to scatter resulting in a blurry image.

An RN is working at an urban low-income medical clinic when a young woman enters crying. She is 19 years old and 28 weeks pregnant with her second child. The woman reports that she woke this morning to find she was leaking milky-colored fluid vaginally. Her first child was born 6 weeks early due to premature rupture of membranes and she is worried this is happening again. You reassure the patient, explain that a vaginal speculum exam will be performed, and educate her about specimens that will be collected. Once the proper specimens are obtained and appropriately labeled, the wet mount and culturette are sent to the laboratory for processing. -The RN understands that along with a pH test,a microscopic view is needed to perform the ferning test to detect an amniotic fluid leak.The patient sample is prepared on a glass slide and examined under 10x magnification.Which type of microscope will be used to make this observation of the patient sample?

Differential media results in which of the following growth characteristics?

A confocal microscope uses visible light as its source of illumination.

The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is ________.

The purpose of staining cells on a microscope slide is to ________.

Mixed cultures are also referred to as contaminated cultures.

Which of the following is not a benefit of agar as a solid medium?

Newly inoculated cultures must be ________ at a specific temperature to encourage growth.

Comparing defined vs.complex media is analogous to comparing ________.

Which method often results in colonies developing down throughout the agar along with some colonies on the surface?

What type of isolation technique is most effective for the majority of applications?

A common medium used for growing fastidious bacteria is ________.

A 65-year-old homeless male presents to an urgent care clinic with a deep laceration on his left arm. He states that he cut his arm on an old piece of scrap metal two days prior. His wound is red, tender, hot to the touch, and has yellow drainage. The RN collects a sample of the drainage and sends it to the laboratory for microbial analysis, per provider orders. -Microscopic analysis revealed the presence of grapelike clusters of gram-positive cocci.The RN educates the patient that the species identification of the organism will require biochemical testing,which aids in microbial identification by providing information on cellular metabolism.Which of the following statements by the patient demonstrates understanding of the nurse's teaching?

For which bacterial genus does mannitol salt agar differentiate between species?

The real image is the reverse of the actual specimen.

If a microbiologist is studying a specimen at a total magnification of 950x,what is the magnifying power of the objective lens if the ocular lens is 10x?

A microbiologist must culture a patient's feces for intestinal pathogens.Which of the following would likely be present in selective media for analyzing this fecal specimen?