Question 1

Gram-negative microbes are surrounded by two layers of __________, between which lies a periplasmic space.

Accepted Answer

A)  peptidoglycan 
B)  membrane 
C)  lipopolysaccharide 
D)  cellulose 
E)  chitin 
A)  peptidoglycan 
B)  membrane 
C)  lipopolysaccharide 
D)  cellulose 
E)  chitin

Question 2

The charging of tRNAs is carried out by a set of enzymes called __________ transferases.

Accepted Answer

A)  ribozymes 
B)  aminoacyl-tRNA 
C)  peptidyl 
D)  proteases 
E)  polymerases 
A)  ribozymes 
B)  aminoacyl-tRNA 
C)  peptidyl 
D)  proteases 
E)  polymerases

Question 3

Which drug intercalates into DNA and inhibits transcription in both prokaryotes and eukaryotes?

Accepted Answer

A)  actinomycin D 
B)  chloramphenicol 
C)  rifampin 
D)  streptomycin 
E)  tetracycline 
A)  actinomycin D 
B)  chloramphenicol 
C)  rifampin 
D)  streptomycin 
E)  tetracycline

Question 4

Peptidyl transferase is present in the __________ ribosomal subunit.

Accepted Answer

A)  30S 
B)  50S 
C)  5S 
D)  16S 
E)  80S 
A)  30S 
B)  50S 
C)  5S 
D)  16S 
E)  80S

Question 5

Describe the experiment utilizing an E. coli cell lysate that was used to figure out the genetic code.

Accepted Answer

Synthetic RNA molecules were employed in

Question 6

Every mRNA has three possible reading frames. How does the ribosome find the correct reading frame?

Accepted Answer

Each mRNA has sequences upstream of the

Question 7

What is the importance of the stop codon UAG?

Accepted Answer

A)  It tells the DNA polymerase to stop replicating the chromosome. 
B)  It tells the RNA polymerase to stop transcribing the chromosome. 
C)  It tells the tRNA to stop transcribing the DNA. 
D)  It tells the ribosome to stop translating the mRNA. 
E)  It signals the end of the operon. 
A)  It tells the DNA polymerase to stop replicating the chromosome. 
B)  It tells the RNA polymerase to stop transcribing the chromosome. 
C)  It tells the tRNA to stop transcribing the DNA. 
D)  It tells the ribosome to stop translating the mRNA. 
E)  It signals the end of the operon.

Question 8

In eukaryotes, protein degradation occurs through organelles called __________.

Accepted Answer

A)  proteases 
B)  lysosomes 
C)  RNases 
D)  proteasomes 
E)  vacuoles 
A)  proteases 
B)  lysosomes 
C)  RNases 
D)  proteasomes 
E)  vacuoles

Question 9

Define "half-life" in terms of mRNA. Biologically, why is it important that the half-life of mRNA be the shortest of the RNAs? What chemically leads to a longer half-life for tRNAs and rRNAs?

Accepted Answer

The half-life of mRNA is the time it tak

Question 10

The average half-life for mRNA in E. coli is:

Accepted Answer

A)  10-30 seconds 
B)  30-60 seconds 
C)  1-3 minutes 
D)  3-5 minutes 
E)  5-10 minutes 
A)  10-30 seconds 
B)  30-60 seconds 
C)  1-3 minutes 
D)  3-5 minutes 
E)  5-10 minutes

Gram-negative microbes are surrounded by two layers of __________, between which lies a periplasmic space.

The charging of tRNAs is carried out by a set of enzymes called __________ transferases.

Which drug intercalates into DNA and inhibits transcription in both prokaryotes and eukaryotes?

Peptidyl transferase is present in the __________ ribosomal subunit.

Describe the experiment utilizing an E. coli cell lysate that was used to figure out the genetic code.

Every mRNA has three possible reading frames. How does the ribosome find the correct reading frame?

What is the importance of the stop codon UAG?

In eukaryotes, protein degradation occurs through organelles called __________.

Define "half-life" in terms of mRNA. Biologically, why is it important that the half-life of mRNA be the shortest of the RNAs? What chemically leads to a longer half-life for tRNAs and rRNAs?

The average half-life for mRNA in E. coli is:

Microbial Life: Origin and Discovery

Observing the Microbial Cell

Cell Structure and Function

Bacterial Culture, Growth, and Development

Environmental Influences and Control of Microbial Growth

Virus Structure and Function

Genomes and Chromosomes

Gene Transfer, Mutations, and Genome Evolution

Molecular Regulation

Viral Molecular Biology

Molecular Techniques and Biotechnology

Energetics and Catabolism

Respiration, Lithotrophy, and Photolysis

Biosynthesis

Food and Industrial Microbiology

Origins and Evolution

Bacterial Diversity

Archaeal Diversity

Eukaryotic Diversity

Microbial Ecology

Microbes and the Global Environment

Human Microbiota and Nonspecific Host Defenses

The Adaptive Immune Response

Microbial Pathogenesis

Microbial Diseases

Antimicrobial Chemotherapy

Clinical Microbiology and Epidemiology

Filters

Exam 8: Transcription, Translation, and Bioinformatics

Gram-negative microbes are surrounded by two layers of __________, between which lies a periplasmic space.

The charging of tRNAs is carried out by a set of enzymes called __________ transferases.

Which drug intercalates into DNA and inhibits transcription in both prokaryotes and eukaryotes?

Peptidyl transferase is present in the __________ ribosomal subunit.

Describe the experiment utilizing an E. coli cell lysate that was used to figure out the genetic code.

Every mRNA has three possible reading frames. How does the ribosome find the correct reading frame?

What is the importance of the stop codon UAG?

In eukaryotes, protein degradation occurs through organelles called __________.

Define "half-life" in terms of mRNA. Biologically, why is it important that the half-life of mRNA be the shortest of the RNAs? What chemically leads to a longer half-life for tRNAs and rRNAs?

The average half-life for mRNA in E. coli is:

Microbial Life: Origin and Discovery

Observing the Microbial Cell

Cell Structure and Function

Bacterial Culture, Growth, and Development

Environmental Influences and Control of Microbial Growth

Virus Structure and Function

Genomes and Chromosomes

Gene Transfer, Mutations, and Genome Evolution

Molecular Regulation

Viral Molecular Biology

Molecular Techniques and Biotechnology

Energetics and Catabolism

Respiration, Lithotrophy, and Photolysis

Biosynthesis

Food and Industrial Microbiology

Origins and Evolution

Bacterial Diversity

Archaeal Diversity

Eukaryotic Diversity

Microbial Ecology

Microbes and the Global Environment

Human Microbiota and Nonspecific Host Defenses

The Adaptive Immune Response

Microbial Pathogenesis

Microbial Diseases

Antimicrobial Chemotherapy

Clinical Microbiology and Epidemiology

Filters