Question 1

The genome of cancer cells tends to become unstable.

Accepted Answer

A) True 
 B)False

Question 2

Why is the Taq DNA polymerase well-suited for PCR?

Accepted Answer

Taq DNA polymerase is a heat-stable enzy

Question 3

If you are setting up a PCR reaction,how much extension time per cycle should you allocate for the Taq DNA polymerase to amplify a 5-kb DNA fragment from a 10-kb template?

Accepted Answer

A) 1 minute 
B) 2.5 minutes 
C) 5 minutes 
D) 10 minutes 
E) 20 minutes 
A) 1 minute 
B) 2.5 minutes 
C) 5 minutes 
D) 10 minutes 
E) 20 minutes

Question 4

A genomic region can rapidly become very common in a population due to

Accepted Answer

A) positive selection. 
B) negative selection. 
C) specific selection. 
D) linkage equilibrium. 
E) homologous recombination. 
A) positive selection. 
B) negative selection. 
C) specific selection. 
D) linkage equilibrium. 
E) homologous recombination.

Question 5

RNA interference functions by

Accepted Answer

A) degrading the protein encoded by a specific mRNA. 
B) degrading the RNA strand that is encoded by the Slicer gene. 
C) transcribing double-stranded RNA into protein. 
D) disrupting the chromosomal copy of the gene. 
E) degrading the RNA complementary to the short,double-stranded RNA. 
A) degrading the protein encoded by a specific mRNA. 
B) degrading the RNA strand that is encoded by the Slicer gene. 
C) transcribing double-stranded RNA into protein. 
D) disrupting the chromosomal copy of the gene. 
E) degrading the RNA complementary to the short,double-stranded RNA.

Question 6

Which of the following is not used in PCR amplification of DNA?

Accepted Answer

A) Double-stranded DNA template 
B) Oligonucleotide primers 
C) DNA polymerase 
D) Buffer 
E) ddNTPs 
A) Double-stranded DNA template 
B) Oligonucleotide primers 
C) DNA polymerase 
D) Buffer 
E) ddNTPs

Question 7

Which of the following does not occur during PCR?

Accepted Answer

A) Denaturation at high temperatures 
B) Synthesis of oligonucleotide primers 
C) Annealing of oligonucleotide primers 
D) Extension of primers by DNA polymerase 
E) Changes in the reaction temperature by a thermocycler 
A) Denaturation at high temperatures 
B) Synthesis of oligonucleotide primers 
C) Annealing of oligonucleotide primers 
D) Extension of primers by DNA polymerase 
E) Changes in the reaction temperature by a thermocycler

Question 8

The human-accelerated region 1 (HAR-1)gene

Accepted Answer

A) was identified by functional genomics. 
B) is identical to the chimpanzee HAR-1. 
C) encodes for the human accelerated region-1 protein. 
D) is a small,noncoding RNA expressed in the brain. 
E) is a small,regulatory RNA that regulates gene expression. 
A) was identified by functional genomics. 
B) is identical to the chimpanzee HAR-1. 
C) encodes for the human accelerated region-1 protein. 
D) is a small,noncoding RNA expressed in the brain. 
E) is a small,regulatory RNA that regulates gene expression.

Question 9

What are the different methods by which we can assign function to particular genes?

Accepted Answer

Functions may be assigned to particular

Question 10

How can we explain the fact that the human genome contains far fewer genes than was predicted would be necessary for our survival?

Accepted Answer

Although we currently do not know the co

Question 11

Sequence similarity between genes plays an important role in assigning gene function.

Accepted Answer

A) True 
 B)False

Question 12

Why are BLAST comparisons based on protein sequences often easier to interpret than those based on DNA sequences?

Accepted Answer

DNA sequences often have great

Question 13

Pharmacogenomics is the study of how

Accepted Answer

A) the genome affects responses to drugs. 
B) the genome leads to new drug discoveries. 
C) the genome affects microbial communities in the body. 
D) the genome is affected by drug use. 
E) medicines influence the genome of cancer cells. 
A) the genome affects responses to drugs. 
B) the genome leads to new drug discoveries. 
C) the genome affects microbial communities in the body. 
D) the genome is affected by drug use. 
E) medicines influence the genome of cancer cells.

Question 14

The drug ________ is added to mouse cell cultures to select against transformants that do not have the desired gene knockout.

Accepted Answer

A) ampicillin 
B) kanamycin 
C) neomycin 
D) G418 
E) ganciclovir 
A) ampicillin 
B) kanamycin 
C) neomycin 
D) G418 
E) ganciclovir

Question 15

Match between columns
                        Premises: HAR-1
shRNA
Transgene
Cy5
BLAST

Accepted Answer

The Answer of Fluorescent dye and Small noncoding human RNA is...

Question 16

What does a large haplotype block suggest about a genomic region?

Accepted Answer

A large haplotype block usually indicate

Question 17

Imagine that you are using random PCR primers to amplify fragments of DNA.Would you expect to get more amplification products if you set the PCR machine at a higher or lower annealing temperature and why?

Accepted Answer

Higher annealing temperatures increase "

Question 18

Why are the number of proteins in the eukaryotic proteome so much greater than the number of eukaryotic genes?

Accepted Answer

Eukaryotic translation is characterized

Question 19

The transcriptome is the complete set of polypeptides produced by an organism.

Accepted Answer

A) True 
 B)False

Question 20

After how many PCR cycles,starting from a single starting molecule,will fragments consisting of only the target DNA (the DNA between the primers)be generated?

Accepted Answer

Fragments consisting of only t

Exam 9: Functional and Comparative Genomics

The genome of cancer cells tends to become unstable.

Why is the Taq DNA polymerase well-suited for PCR?

If you are setting up a PCR reaction,how much extension time per cycle should you allocate for the Taq DNA polymerase to amplify a 5-kb DNA fragment from a 10-kb template?

A genomic region can rapidly become very common in a population due to

RNA interference functions by

Which of the following is not used in PCR amplification of DNA?

Which of the following does not occur during PCR?

The human-accelerated region 1 (HAR-1)gene

What are the different methods by which we can assign function to particular genes?

How can we explain the fact that the human genome contains far fewer genes than was predicted would be necessary for our survival?

Sequence similarity between genes plays an important role in assigning gene function.

Why are BLAST comparisons based on protein sequences often easier to interpret than those based on DNA sequences?

Pharmacogenomics is the study of how

The drug ________ is added to mouse cell cultures to select against transformants that do not have the desired gene knockout.

What does a large haplotype block suggest about a genomic region?

Imagine that you are using random PCR primers to amplify fragments of DNA.Would you expect to get more amplification products if you set the PCR machine at a higher or lower annealing temperature and why?

Why are the number of proteins in the eukaryotic proteome so much greater than the number of eukaryotic genes?

The transcriptome is the complete set of polypeptides produced by an organism.

After how many PCR cycles,starting from a single starting molecule,will fragments consisting of only the target DNA (the DNA between the primers)be generated?

Genetics: An Introduction

DNA: The Genetic Material

DNA Replication

Gene Function

Gene Expression: Transcription

Gene Expression: Translation

Dna Mutation, DNA Repair, and Transposable Elements

Genomics: The Mapping and Sequencing of Genomes

Recombinant DNA Technology

Mendelian Genetics

Chromosomal Basis of Inheritance

Extensions of and Deviations From Mendelian Genetic Principles

Genetic Mapping in Eukaryotes

Genetics of Bacteria and Bacteriophages

Variations in Chromosome Structure and Number

Regulation of Gene Expression in Bacteria and Bacteriophages

Regulation of Gene Expression in Eukaryotes

Genetic Analysis of Development

Genetics of Cancer

Population Genetics

Quantitative Genetics

Molecular Evolution

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