Question 1

The molecule(s) that act as molecular glue to bind DNA fragments together is/are

Accepted Answer

A)  DNAse. 
B)  DNA ligase. 
C)  ligandase. 
D)  polymerase. 
E)  DNAse AND ligandase. 
A)  DNAse. 
B)  DNA ligase. 
C)  ligandase. 
D)  polymerase. 
E)  DNAse AND ligandase. B

Question 2

The current cost of sequencing a human genome is about

Accepted Answer

A)  $ 10,000 
B)  $ 250,000 
C)  $ 1,000,000 
D)  $ 4,000,000 
A)  $ 10,000 
B)  $ 250,000 
C)  $ 1,000,000 
D)  $ 4,000,000 D

Question 3

When a vector that employs the lacZ gene as a second marker is used in a cloning experiment, bacteria that harbor the recombinant DNA will give rise to

Accepted Answer

A)  red colonies. 
B)  white colonies. 
C)  blue colonies. 
D)  All of the choices are correct. 
A)  red colonies. 
B)  white colonies. 
C)  blue colonies. 
D)  All of the choices are correct. B

Question 4

A DNA microarray contains oligonucleotides that contain a label.

Accepted Answer

A) True 
 B)False

Question 5

When using lacZ containing vectors, colonies containing intact vector turn blue.

Accepted Answer

A) True 
 B)False

Question 6

Double-stranded DNA will separate into two strands when exposed to

Accepted Answer

A)  high temperature. 
B)  high pH. 
C)  low temperature. 
D)  low salt. 
E)  high temperature AND high pH. 
A)  high temperature. 
B)  high pH. 
C)  low temperature. 
D)  low salt. 
E)  high temperature AND high pH.

Question 7

Which of the following genera has proved useful for manipulating plant cells?

Accepted Answer

A)  Escherichia 
B)  Bacillus 
C)  Pseudomonas 
D)  Agrobacterium 
A)  Escherichia 
B)  Bacillus 
C)  Pseudomonas 
D)  Agrobacterium

Question 8

A common way to identify the

Accepted Answer

A)  vector. 
B)  probe. 
C)  host. 
D)  plasmid. 
E)  coli that carries the desired recombinant DNA is by using a 
A)  vector. 
B)  probe. 
C)  host. 
D)  plasmid. 
E)  coli that carries the desired recombinant DNA is by using a

Question 9

A danger in using E. coli in cloning is that

Accepted Answer

A) E. coli could cause disease. 
B)  the human cells may reject the insertion. 
C)  the exons may invert the introns. 
D)  the outer membrane is poisonous to humans. 
A) E. coli could cause disease. 
B)  the human cells may reject the insertion. 
C)  the exons may invert the introns. 
D)  the outer membrane is poisonous to humans.

Question 10

An ideal vector

Accepted Answer

A)  may be a plasmid or bacteriophage. 
B)  has a restriction enzyme recognition site. 
C)  contains an origin of replication. 
D)  contains a selectable marker. 
E)  All of the choices are correct. 
A)  may be a plasmid or bacteriophage. 
B)  has a restriction enzyme recognition site. 
C)  contains an origin of replication. 
D)  contains a selectable marker. 
E)  All of the choices are correct.

Question 11

The entire set of cloned fragments of the complete human genome is termed a

Accepted Answer

A)  book of genes. 
B)  recombinant gene. 
C)  DNA library. 
D)  restructured genome. 
A)  book of genes. 
B)  recombinant gene. 
C)  DNA library. 
D)  restructured genome.

Question 12

The size of the amplified DNA fragment generated during PCR is determined by

Accepted Answer

A)  how many cycles are performed. 
B)  the size of the template DNA. 
C)  the location to which the primers anneal. 
D)  how much Taq is used. 
A)  how many cycles are performed. 
B)  the size of the template DNA. 
C)  the location to which the primers anneal. 
D)  how much Taq is used.

Question 13

Starting with a single piece of dsDNA, after 3 PCR cycles there are

Accepted Answer

A)  2 additional pieces of dsDNA. 
B)  4 additional pieces of dsDNA. 
C)  8 additional pieces of dsDNA. 
D)  16 additional pieces of dsDNA. 
A)  2 additional pieces of dsDNA. 
B)  4 additional pieces of dsDNA. 
C)  8 additional pieces of dsDNA. 
D)  16 additional pieces of dsDNA.

Question 14

The agarose used in electrophoresis

Accepted Answer

A)  interacts electrically with the DNA. 
B)  chemically binds to the DNA. 
C)  acts as a sieve. 
D)  selectively sorts recombinant DNA from host DNA. 
A)  interacts electrically with the DNA. 
B)  chemically binds to the DNA. 
C)  acts as a sieve. 
D)  selectively sorts recombinant DNA from host DNA.

Question 15

Mitochondrial DNA (mtDNA) in humans

Accepted Answer

A)  is always donated to the offspring from both parents 
B)  can be used to identify related people 
C)  can be isolated only from intact embryos 
D)  can be used to establish paternity 
A)  is always donated to the offspring from both parents 
B)  can be used to identify related people 
C)  can be isolated only from intact embryos 
D)  can be used to establish paternity

Question 16

Agarose gel electrophoresis may be considered as a partial purification technique.

Accepted Answer

A) True 
 B)False

Question 17

Genetic engineering of plants has so far produced

Accepted Answer

A)  pest resistant plants. 
B)  plants that are herbicide resistant. 
C)  plants with increased nutritional value. 
D)  All of the choices are correct. 
A)  pest resistant plants. 
B)  plants that are herbicide resistant. 
C)  plants with increased nutritional value. 
D)  All of the choices are correct.

Question 18

The polymerase chain reaction is used to

Accepted Answer

A)  amplify certain sections of DNA. 
B)  amplify mRNA. 
C)  produce proteins. 
D)  produce long polymers of carbohydrates to be used in electrophoresis. 
A)  amplify certain sections of DNA. 
B)  amplify mRNA. 
C)  produce proteins. 
D)  produce long polymers of carbohydrates to be used in electrophoresis.

Question 19

Vectors must have at least one restriction enzyme recognition site.

Accepted Answer

A) True 
 B)False

Question 20

PCR is particularly useful in

Accepted Answer

A)  detecting viable yet non-culturable organisms. 
B)  assessing impure (multiple types of bacteria present) samples. 
C)  dealing with very small numbers of bacteria. 
D)  relatively quickly producing results. 
E)  All of the choices are correct. 
A)  detecting viable yet non-culturable organisms. 
B)  assessing impure (multiple types of bacteria present) samples. 
C)  dealing with very small numbers of bacteria. 
D)  relatively quickly producing results. 
E)  All of the choices are correct.

Exam 9: Biotechnology and Recombinant Dna

The molecule(s) that act as molecular glue to bind DNA fragments together is/are

The current cost of sequencing a human genome is about

When a vector that employs the lacZ gene as a second marker is used in a cloning experiment, bacteria that harbor the recombinant DNA will give rise to

A DNA microarray contains oligonucleotides that contain a label.

When using lacZ containing vectors, colonies containing intact vector turn blue.

Double-stranded DNA will separate into two strands when exposed to

Which of the following genera has proved useful for manipulating plant cells?

A common way to identify the

A danger in using E. coli in cloning is that

An ideal vector

The entire set of cloned fragments of the complete human genome is termed a

The size of the amplified DNA fragment generated during PCR is determined by

Starting with a single piece of dsDNA, after 3 PCR cycles there are

The agarose used in electrophoresis

Mitochondrial DNA (mtDNA) in humans

Agarose gel electrophoresis may be considered as a partial purification technique.

Genetic engineering of plants has so far produced

The polymerase chain reaction is used to

Vectors must have at least one restriction enzyme recognition site.

PCR is particularly useful in

Humans and the Microbial World

The Molecules of Life

Microscopy and Cell Structure

Dynamics of Prokaryotic Growth

Control of Microbial Growth

Metabolism: Fueling Cell Growth

The Blueprint of Life, From Dna to Protein

Bacterial Genetics

Identification and Classification of Prokaryotes

The Diversity of Prokaryotic Organisms

The Eukaryotic Members of the Microbial World

Viruses, Viroids and Prions

The Innate Immune Response

The Adaptive Immune Response

Host-Microbe Interactions

Immunologic Disorders

Applications of Immune Responses

Epidemiology

Antimicrobial Medications

Respiratory System Infections

Skin Infections

Wound Infections

Digestive System Infections

Genitourinary Infections

Nervous System Infections

Blood and Lymphatic Infections

Hiv Disease and Complications of Immunodeficiency

Microbial Ecology

Environmental Microbiology: Treatment of Water, Wastes, and Polluted Habitats

Food Microbiology

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